Enzymatic transformation of stevioside using a β-galactosidase from Sulfolobus sp.

Abstract

Enzymatic hydrolysis and transgalactosylation of stevioside (St) were investigated using a β-galactosidase from Sulfolobus sp. The hydrolysis yielded steviol as the main final product. Under the optimal transgalactosylation conditions, the highest conversion of stevioside was 87.3% with lactose as a donor, several galactosylated products (St-Gals) were obtained. Metal ions such as Na(+), K(2+), Ca(2+), Ba(2+), Mn(2+) and Mg(2+) (2 mM) did not affect the transgalactosylation activity, while Fe(2+), Fe(3+) and Cu(2+) reduced the transgalactosylation activity of β-galactosidase to 64%, 33% and 18%, respectively.

β-Glucosidase and β-Galactosidase-Mediated Transglycosylation of Steviol Glycosides Utilizing Industrial Byproducts

“…However, Tt bGal1 was not able to hydrolyze the above-mentioned bonds, since no hydrolysis products were detected when the enzyme was incubated with either stevioside or RebA in the absence of donor, and therefore it was shown to be an ideal catalyst for the transgalactosylation of both stevioside and RebA. On the other hand, a β-galactosidase from Sulfolobus sp., was found to both hydrolyze stevioside toward the production of steviol, and, with the addition of lactose as donor, transgalactosylate stevioside reaching up to 87.3% stevioside conversion ( Wan and Xia, 2015 ).…”

Simultaneous improvement to solubility and bioavailability of active natural compound isosteviol using cyclodextrin metal-organic frameworks

“…However, one drawback of STV's application as medicine could be its low solubility and low bioavailability 13 . At present, methods to improve the solubility of STV include: solid dispersion technology 14 , 15 , the use of enzymes such as transglycosylase to improve solubility through regioselective enzymatic glycosylation 16 , 17 , 18 , 19 , complexation with γ -cyclodextrin ( γ -CD) 20 , etc. There was no report on using the cyclodextrin metal-organic framework (CD-MOF) encapsulation of STV.…”

RQ3, A Natural Rebaudioside D Isomer, Was Obtained from Glucosylation of Rebaudioside A Catalyzed by the CGTase Toruzyme 3.0 L

“…For example, rebaudioside D (Figure 1s) and rebaudioside M, which carry two and three C-19 linked glycosyl units, respectively, both demonstrate improved edulcorant quality over that of stevioside or rebaudioside A. Hence, enzymatic glucosylation of stevioside and rebaudioside A have been extensively studied − to improve the edulcorant quality; ,− the resultant products are so-called glucosyl steviol glycosides (GSGs).…”