Enzymatic Treatment of Polyamide Fiber by Alcalase

Song, Yu-Sun; Song, Wha-Soon

doi:10.5850/jksct.2011.35.8.1006

Cited by 5 publications

(5 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These activity assays were carried out in different treatment conditions. The activity was converted to relative activity (percentage of the maximum activity obtained in that series) for both the free and immobilized laccase as follows 21,22 Relative activity %…”

Section: Iymentioning

confidence: 99%

“…3 ) that can be readily measured at 340 nm. 16,21,22,30,39 Figures 4(A)-(C) show the hydrolytic activity of each protease on polyamide fabric as assessed through RH (%), the K/S value after wool-reactive dyeing and the WCA ( ), respectively. As shown in Figure 4(A), the highest RH (%) of about 73% was obtained from bromelain hydrolysis.…”

Section: Enzymatic Hydrolysismentioning

confidence: 99%

“…The free amino groups released into the treatment solution formed complexes with TNBS. 16,21,22,30,39 When the number complexes between free amino groups and TNBS increased in the treatment solution after bromelain hydrolysis, the absorbance also increased. 16,17 Thus, the highest value of RH (%) demonstrated that bromelain was capable of hydrolyzing amide bonds in polyamide fabric.…”

Section: Enzymatic Hydrolysismentioning

confidence: 99%

“…Polyamide surfaces typically have been modified through activation by ultraviolet (UV) irradiation, 17 plasma treatment, 18 chemical partial hydrolysis 19 and enzymatic modification. 15,[20][21][22][23] Since the environmental issues have increased interest, the enzymatic hydrolysis on the polyamide surface has been focused upon in textile industries. [15][16][17][18][19][20][21][22][23][24] Enzymatic treatments are conducted under mild conditions and can produce similar or even better results for the surface modification of synthetic fibers.…”

mentioning

confidence: 99%

“…15,[20][21][22][23] Since the environmental issues have increased interest, the enzymatic hydrolysis on the polyamide surface has been focused upon in textile industries. [15][16][17][18][19][20][21][22][23][24] Enzymatic treatments are conducted under mild conditions and can produce similar or even better results for the surface modification of synthetic fibers. [25][26][27][28] Through enzymatic hydrolysis, the amino (NH 2 ) and carboxyl groups (COOH) are created on polyamide fiber by the cleavage of the amide bond (-CONH-).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Effect of enzymatic hydrolysis on developing support of polyamide woven fabric for enzyme immobilization

Song

Kim

Lee

2018

Textile Research Journal

View full text Add to dashboard Cite

Polyamide fiber has been considered as a suitable support for enzyme immobilization because of its low cost, chemical and mechanical properties and ready availability in a number of different forms. In particular, polyamide fabric has highly specific surface and good elasticity. The present study aims to develop an immobilization support from polyamide fabric and to establish the optimum immobilization conditions for laccase. For this, the enzymatic hydrolysis process was introduced to the hydrolysis of polyamide, creating amino groups that immobilize enzyme molecules. When polyamide fabric was hydrolyzed by bromelain during enzymatic hydrolysis, the highest immobilization yield (68 ± 0.7%) and relative activity (95 ± 0.52%) of immobilized laccase were obtained. For successful enzyme immobilization, the optimal glutaraldehyde crosslinking conditions were pH of 9.0 with 10% (v/v) of glutaraldehyde concentration for 240 min at 45℃. The most favorable immobilization conditions were as follows: pH of 6.0 with 35% (owf) of laccase concentration for 600 min at 4℃. Under the optimum treatment conditions, the pH and thermal stability of immobilized laccase were improved. After 20 days of storage, the immobilized laccase on enzymatic hydrolyzed polyamide fabric retained approximately 30% of its initial activity. Furthermore, the immobilized laccase indicated potential reuse over 10 use cycles. The structural changes of polyamide fabric according to treatment processes were demonstrated by Fourier transform infrared spectroscopy. The changes of surface morphology were measured by scanning electron microscopy according to the multi treatment steps.

show abstract

Section: Iymentioning

confidence: 99%