Enzymatic versus Electrocatalytic Oxidation of NADH at Carbon‐Nanotube Electrodes Modified with Glucose Dehydrogenases: Application in a Bucky‐Paper‐Based Glucose Enzymatic Fuel Cell

Abstract

International audienc

Buckypaper bioelectrodes: emerging materials for implantable and wearable biofuel cells

“…97 In later work, the bioanode with immobilized cofactor was used for the construction of a paper-based biofuel cell flow device. 97,116 Reid et al 122,123 In the work by Reid et al, commercial MWCNT buckypaper (27 gsm, 87% porosity, 2.07 Ωsq -1 ) was modified with polymethylene green, NAD-dependent lactate dehydrogenase from Escherichia coli and NAD + cofactor. In addition, the electrode was modified with a polyethyleneimene hydrogel and an ethylene glycol diglycidyl ether crosslinker to entrap and stabilize the cofactor and enzyme on the surface whilst allowing efficient permeation of the electrolyte and fuel.…”

“…Either a pyrene-modified Ru complex or diaphorase from Clostridium kluyveri was immobilized on the electrode to facilitate catalytic glucose oxidation. 123 The diaphorase bioanode had the advantage of low overpotentials for NADH oxidation whereas the Ru complex bioanode exhibited higher catalytic currents.…”

Molecular Release Associated with Interfacial pH Change Stimulated by a Small Electrical Potential Applied

“…The electron transport proceeds as electrochemical oxidation of NADH at the buckypaper electrode, followed by the electron transport to the immobilized BOx through the conducting support. Similar electrochemical transformations have been reported for buckypaper electrodes modified with different enzymes . An open circuit potential (OCP) measured on the modified electrode in the presence of 2 mM NADH was ca.…”

“…Similar electrochemical transformations have been reported for buckypaper electrodes modified with different enzymes. [26] An open circuit potential (OCP) measured on the modified electrode in the presence of 2 mM NADH was ca. À 20 mV (vs. Ag/AgCl) reflecting the NADH Figure 1B; note that this curve is identical to one shown in Figure 3B the release process in the presence of NADH.…”

Nanomaterial-Based Electrochemical and Colorimetric Sensors for On-Site Detection of Small-Molecule Targets

“…Recently, SWCNT-based "Bucky-paper" was used as a new substrate for NADH oxidation. 106 However, it suffers from very high background due to the thickness of the paper (~350 µm), and the SWCNTs must be modified with an electrocatalyst for NADH oxidation. However, these films have rarely been applied in the context of detection in biosamples.…”