Enzymatically cross-linked gelatin-phenol hydrogels with a broader stiffness range for osteogenic differentiation of human mesenchymal stem cells

Wang, Lishan; Du, Chan; Chung, Joo Eun; Kurisawa, Motoichi

doi:10.1016/j.actbio.2012.02.002

Cited by 103 publications

(91 citation statements)

References 36 publications

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“…For example, there is significant interest in the use of hydrogel/stem cell composites for bone repair. However, the stiffness of hydrogels does not approach the high modulus of bone tissue [73] and may therefore limit the osteogenic potential of the cells encapsulated within them.…”

Section: Future Perspectives Modulating Mirna Signaling To Overcome Lmentioning

confidence: 99%

Concise Review: New Frontiers in MicroRNA-Based Tissue Regeneration

Frith

Porrello

Cooper-White

2014

Stem Cells Translational Medicine

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This review highlights the role of microRNAs (miRNAs) in tissue regeneration, discusses key challenges in translating this knowledge to the clinic, and outlines recent technological advances that aim to address these issues. By combining a comprehensive knowledge of miRNA biology with cutting‐edge delivery technologies, it is clear that miRNAs hold significant promise for tissue regenerative therapies in the future.

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Section: Future Perspectives Modulating Mirna Signaling To Overcome Lmentioning

confidence: 99%

Concise Review: New Frontiers in MicroRNA-Based Tissue Regeneration

Frith

Porrello

Cooper-White

2014

Stem Cells Translational Medicine

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“…At day 1, cells are proliferating in the hydrogels, and developed a narrow actin cytoskeleton showing a fairly round shape as typically occurs in cells encapsulated in low stiffness hydrogels for this short period of time [186]- [188]. The cellular growth indicates low toxicity of enzymatically cross-linked hydrogels as reported by previous studies where the compatibility, spreading and cell attachment onto the surface of enzymatically crosslinked GEL-TA was evaluated [129], [189]. After 21 days of culture (Figure 3-7), cells proliferated and achieved confluence, showing a round actin cytoskeleton shape.…”

Section: Cell Culture and Seedingmentioning

confidence: 63%

“…It is not formed by a single chemical entity but a mixture of fractions composed of amino acids joined by peptide linkages to form polymers varying in molecular mass from 10000 to 400000 g/mol [89]. It is enzymatically degraded by collagenases, which degrade the gelatin chain cleaving the peptide bonds [5], [90], [91].…”

Section: Gelatinmentioning

confidence: 99%

“…Previous cytotoxicity studies in this type of in situ crosslinking gelatin have demonstrated the non-cytotoxicity of the system [129], [189]. Our purpose was to analyze the non-cytotoxic effect of the PLLA-PHEMA grafted fibers in view of the application of these new injectable composites in tissue engineering.…”

Section: Cytotoxicity Of the Compositesmentioning

confidence: 99%

“…The degradation dynamics of Gel/HA hydrogels was studied by enzymatic degradation with a 10 U/ml solution of hyaluronidase and 3 U/mL of collagenase in DPBS, these concentrations being within the range typically used in the bibliography [90], [91], [114], [131], [225], [226]. A mixture of both enzymes was used to study degradation, since it has been demonstrated that the combination of both produces higher degradation rates than when only one is used [69].…”

Section: Enzymatic Degradation Of Gel/ha Hydrogelsmentioning

confidence: 99%

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Protein-based injectable hydrogels towards the regeneration of articular cartilage

Poveda-Reyes¹

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A mis seres queridos, con ellos todo es posible "The future belongs to those who believe in the beauty of their dreams"Eleanor Roosevelt Acknowledgements 7 AcknowledgementsAfter reaching this point I would like to thank everybody that in one way or another has helped me through these four years of work.First of all, I gratefully acknowledge the financial support from the Spanish Ministry of Economy and Competitiveness through the DPI2010-20399-C04-03 and MAT2013-46467-C4-1-R projects, my FPI fellowship BES-2011-046144 and the EEBB-I-13-06179 and EEBB-I-14-08725 research stay grants.I would especially like to thank my thesis supervisor, Dr. Gloria Gallego Ferrer, for helping me in everything I needed through these four years, for giving me this opportunity and for all her efforts in my assistance. Also thanks to José Luis Gómez Ribelles for his valuable help and brilliant ideas.Thanks to the staff of the Microscopy Service at the Universitat Politècnica de València, for their guidance and help during the SEM sessions.I would also like to express my gratitude to my supervisors during my stays at other Research Centers. Thanks to Prof. Ulrica Edlund, who during my stay at the Fiber and Polymer Technology Department (KTH) in Stockholm, helped me with everything I could need and to all the people in the center (Laleh, Anas, Soheil, Bekir, Parmida, Robertus, Arturo, etc.) that helped me in the lab and made my stay easier. Thanks to Prof. Manuel Salmerón Sánchez at the Microenvironments for Medicine group (MiMe) (University of Glasgow) for giving me the opportunity to learn the basics of cell culture in his lab, his guidance and valuable help. I would also like to thank Dr. Vladimira Moulisova for teaching me everything I know of cell biology work and being so patient and friendly. And to all the people of the group (Marco, Sara, Eleni, Mark, Alex, Jake, Elie, Frankie, etc.), as they say "People make Glasgow" and they made me feel at home. I cannot forget all the people at the Center for Biomaterials and Tissue Engineering: to Laura Teruel, for being so patient with everyone, so helpful and keeping the lab in a perfect state, and all the lecturers for their help and advice, especially to Ana Vallés and Manuel Monleón, who introduced me to the wonderful field of Tissue Engineering.I would like to especially thank Tatiana C. Gamboa, who guided me in my first years in the lab. To Suhail, Rocío, Marta, Félix, Petra, Leonardo, Rebeca and Esther, I helped them in their first steps in the lab but I learnt a lot from them and they helped me a lot, both professional and personally. Also thanks to Alex Rodrigo who helped me a lot in my first cell cultures even if he had a million things to do.Thanks to all the people who have been in the "Sala de Reuniones" office: Manu, María, Line, Amparo G, Roberto, Guillermo, Álvaro, Laia, Rubén, etc., thanks for the break times, your laughs, your help and being the way you are.Special thanks to my friends, they gave me support through all these years, with them life is better.Finally, I w...

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