Enzymatically validated liquid chromatographic method for the determination of ascorbic and dehydroascorbic acids in fruit and vegetables

Gökmen, Vural; Kahraman, Nermin; Demir, Nilay; Acar, Jale

doi:10.1016/s0021-9673(00)00080-7

Cited by 161 publications

(122 citation statements)

References 13 publications

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“…The quantity of DTT necessary depended on the DHA content present in the sample. Based on reports from other authors 5,6 and on tests it was concluded that the use of 50 mM DTT did not result in greater conversion than when 10 mM were used. Therefore a 40 mM DTT solution was used diluted in the buffer itself.…”

Section: Optimization Of the Dha Reduction Processmentioning

confidence: 91%

“…The addition of 1 mL DTT (4 or 8 mM) to 1 mL of collard and tomato extract, containing 4.5% MPA, resulted in AA loss or increases of at most 4%, after reaction times of 75, 90 and 120 min, as proposed by Gökmen et al 6 Thus it was attempted to increase the DTT concentration, using a solution at 50 mM, but the results did not change.…”

Section: Optimization Of the Dha Reduction Processmentioning

confidence: 94%

“…The vitamin C extraction methods were tested using the following extracting solutions: only water, as proposed by Gökmen et al, 6 4.5% metaphosphoric acid (MPA) according to the method proposed by Giannakourou et al 9 and extracting solution consisting of 3% MPA, 8% acetic acid, 1 mM EDTA and 0.15 M H 2 SO 4 , based on Franke et al, 8 with some modifications.…”

Section: Vitamin C Extractionmentioning

confidence: 99%

“…Furthermore, the presence of metals such as iron and copper increase AA oxidation 1 and a metal chelating agent is usually recommended. 5 Pure water 6 or acid solutions have been used to extract AA from plant tissues. The acids commonly used include metaphosphoric acid (MPA) 5,[7][8][9][10] and oxalic acid 5,11,12 alone or in combinations with other acids and/or organic solvents, added or not of antioxidants such as EDTA and BHT.…”

Section: Introductionmentioning

confidence: 99%

“…Among the reducing agents dithiothreitol (DTT) has been widely used. 6,19,21,22 The objective of this study was to test the methodology for analyzing AA and DHA in vegetables by reverse phase high performance liquid chromatography, optimizing the extraction processes of ascorbic acid and DHA conversion to ascorbic acid, using dithiothreitol (DTT). In addition, the efficiency of the optimized methodology was verified in vegetables with the addition of oil.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of methodology to analyze ascorbic and dehydroascorbic acid in vegetables

Campos¹,

Ribeiro²,

Lúcia³

et al. 2009

Quím. Nova

111

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OptimizatiOn Of methOdOlOgy tO analyze ascOrbic and dehydrOascOrbic acid in vegetables flávia milagres campos*, sônia machado rocha ribeiro, ceres m. della lucia e helena maria pinheiro-sant'ana Departamento de Nutrição e Saúde, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, 36571-000 Viçosa -MG, Brasil paulo césar stringheta Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Av. PH Rolfs, s/n, 36571-000 Viçosa -MG, BrasilRecebido em 10/1/08; aceito em 18/8/08; publicado na web em 15/12/08In this study, different solutions to extract vitamin C were tested. High-performance liquid chromatography was chosen and the conditions were based on isocratic elution in reverse phase column. Dehydroascorbic acid was determined indirectly after its reduction using dithiothreitol. The use of metaphosphoric acid to stabilize the vitamin C was shown to be required and it was necessary to neutralize the pH of the extract to apply dithiothreitol. The average recovery was 90% in collard and tomato samples. The presence of oil did not interfere in extraction and the methodology can be used to analyze stir fried vegetables.

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Section: Optimization Of the Dha Reduction Processmentioning

confidence: 91%

Section: Optimization Of the Dha Reduction Processmentioning

confidence: 94%

Section: Vitamin C Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Optimization of methodology to analyze ascorbic and dehydroascorbic acid in vegetables

Campos¹,

Ribeiro²,

Lúcia³

et al. 2009

Quím. Nova

111

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Current Awareness

2000

Phytochem. Anal.

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Ascorbic Acid Induced Enhancement of Room Temperature Phosphorescence of Sodium Tripolyphosphate‐Capped Mn‐Doped ZnS Quantum Dots: Mechanism and Bioprobe Applications

Wang¹,

Li²,

Wu³

et al. 2010

Chemistry A European J

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Although quantum dot (QD)-based room temperature phosphorescence (RTP) probes are promising for practical applications in complex matrixes such as environmental, food and biological samples, current QD-based-RTP probes are not only quite limited but also exclusively based on the RTP quenching mechanism. Here we report an ascorbic acid (AA) induced phosphorescence enhancement of sodium tripolyphosphate-capped Mn-doped ZnS QDs, and its application for turn-on RTP detection. The chelating ability allows AA to extract the Mn and Zn from the surface of the QDs and to generate more holes which are subsequently trapped by Mn(2+), while the reducing property permits AA to reduce Mn(3+) to Mn(2+) in the excited state, thereby enhancing the excitation and orange emission of the QDs. The enhanced RTP intensity of the QDs increases linearly with the concentration of AA in the range of 0.05-0.8 μM. Thus, a QD-based RTP probe for AA is developed. The proposed QD-based turn-on RTP probe avoids tedious sample pretreatment, and offers good sensitivity and selectivity for AA in the presence of the main relevant metal ions and other molecules in biological fluids. The limit of detection (3s) of the developed method is 9 nM AA, and the relative standard deviation is 4.8 % for 11 replicate detections of 0.1 μM AA. The developed method is successfully applied to the analysis of real samples of human urine and plasma for AA with quantitative recoveries from 96 to 105 %.

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Enzymatically validated liquid chromatographic method for the determination of ascorbic and dehydroascorbic acids in fruit and vegetables

Cited by 161 publications

References 13 publications

Optimization of methodology to analyze ascorbic and dehydroascorbic acid in vegetables

Optimization of methodology to analyze ascorbic and dehydroascorbic acid in vegetables

Current Awareness

Ascorbic Acid Induced Enhancement of Room Temperature Phosphorescence of Sodium Tripolyphosphate‐Capped Mn‐Doped ZnS Quantum Dots: Mechanism and Bioprobe Applications

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