Enzyme activities and activity profiles in muscle fibers of dystrophic, immature‐normal, and adult‐normal BL6 mice

Reichmann, Heinz; Pette, Dirk

doi:10.1002/mus.880070206

Cited by 16 publications

(2 citation statements)

References 26 publications

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“…Seit ca. 15 Jahren erlebten die experimentelle Elektrostimulation und der klinische Einsatz weltweit unter dem Oberbegriff "Funktionelle Elektrostimulation" neue Forschungsimpulse, die u. a. von Hudlicka (126)(127)(128)(129)(130)(131), Pette (241)(242)(243), Vrbova (312), Reichmann (252)(253)(254)(255)(256)(257), Hoppeler (119)(120)(121)(122)(123), Lomo (179)(180)(181)(182) u.v.a. von der experimentell-physiologischen Seite und von der klinischen und biotechnischen Seite u.a.…”

Section: Abb 1: Galvani Demonstriert Die Elektrischeunclassified

Functional electrical stimulation on paraplegic patients

Kern

2014

Eur J Transl Myol

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Functional Electrical Stimulation on Paraplegic Patients. We report on clinical and physiological effects of 8 months Functional Electrical Stimulation (FES) of quadriceps femoris muscle on 16 paraplegic patients. Each patient had muscle biopsies, CT-muscle diameter measurements, knee extension strength testing carried out before and after 8 months FES training. Skin perfusion was documented through infrared telethermography and xenon clearance, muscle perfusion was recorded through thallium scintigraphy. After 8 months FES training baseline skin perfusion showed 86 % increase, muscle perfusion was augmented by 87 %. Muscle fiber diameters showed an average increase of 59 % after 8 months FES training. Muscles in patients with spastic paresis as well as in patients with denervation showed an increase in aerob and anaerob muscle enzymes up to the normal range. Even without axonal neurotropic substances FES was able to demonstrate fiberhypertrophy, enzyme adaptation and intracellular structural benefits in denervated muscles. The increment in muscle area as visible on CT-scans of quadriceps femoris was 30 % in spastic paraplegia and 10 % in denervated patients respectively. FES induced changes were less in areas not directly underneath the surface electrodes. We strongly recommend the use of Kern`s current for FES in denervated muscles to induce tetanic muscle contractions as we formed a very critical opinion of conventional exponential current. In patients with conus-cauda-lesions FES must be integrated into modern rehabilitation to prevent extreme muscle degeneration and decubital ulcers. Using FES we are able to improve metabolism and induce positive trophic changes in our patients lower extremities. In spastic paraplegics the functions "rising and walking" achieved through FES are much better training than FES ergometers. Larger muscle masses are activated and an increased heart rate is measured, therefore the impact on cardiovascular fitness and metabolism is much greater. This effectively addresses and prevents all problems which result from inactivity in paraplegic patients.

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Section: Abb 1: Galvani Demonstriert Die Elektrischeunclassified

Functional electrical stimulation on paraplegic patients

Kern

2014

Eur J Transl Myol

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“…Experimental errors resulting from variations in section thickness, differences in temperature between different measurements, etc., are thus eliminated and enzyme activities determined in large fibre numbers can be evaluated as relative activities. Finally, the method of comparative histochemical enzyme activity determination has been successfully applied to studying experimentally induced muscle fibre transformations (Pette & Tyler, 1983;Buchegger et al, 1984) or enzyme profiles in pathological muscles (Pette & Reichmann, 1984b;Dangain et al, 1987;Reichmann, 1988). These studies used cross-sections from composite blocks of normal and experimental muscles, or normal and pathological muscles, in order to compare enzyme activities between fibres at two different conditions.…”

Section: Comparative Measurement Of Enzyme Activities In Single Tissumentioning

confidence: 99%

The principle of determining relative enzyme activities by comparative kinetic microphotometryin situ

Pette

Reichmann²

1989

Histochem J

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Evidence for the identities of five proteins decreased in skeletal muscle of dystrophic mice

Jasch

Moase

1985

Muscle and Nerve

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In a previous study, protein distributions in normal and dystrophic soleus and EDL were examined using polyacrylamide isoelectric focusing. Whereas normally protein distributions in these two muscles were characteristically different, in dystrophic muscles they were strikingly similar. The soleus-specific proteins were identified as the myosin light chains LCls and LC2s. The EDL-amplified proteins were the myosin light chains LC1f, LC2f, the phosphorylated form of LC2f (LC2f-P), and LC3f. In addition, evidence is presented that one protein, which had not been identified in two-dimensional gels, is parvalbumin. The decreased proportion of parvalbumin, LC2f-P, and LC3f in the dystrophic EDL was also shown in other fast-twitch muscles of the mouse. We suggest that these changes in protein distributions in dystrophic muscles reflect a loss of their acquired state of differentiation.

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Enzyme activities and activity profiles in muscle fibers of dystrophic, immature‐normal, and adult‐normal BL6 mice

Cited by 16 publications

References 26 publications

Functional electrical stimulation on paraplegic patients

Functional electrical stimulation on paraplegic patients

The principle of determining relative enzyme activities by comparative kinetic microphotometryin situ

Evidence for the identities of five proteins decreased in skeletal muscle of dystrophic mice

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