Enzyme activity and stability of lactase immobilized on two different supports: Calcium alginate and magnetic chitosan

Abstract

Lactase is a very important enzyme to cure lactose intolerance problem. However, it is naturally existing in soluble form and cannot be reused. The current study was performed to compare the productivity and stability of lactase immobilized on calcium alginate and magnetic chitosan. The reusability of immobilized enzyme was measured for 28 days. Thermal stability was measured at 27, 37, 50 and 70 ºC. Lactase immobilized on calcium alginate showed a better stability after 21 days where it retained up to 62% of enzyme activity. However, lactase on magnetic chitosan expresses a better thermal stability as it produced 6% more sugar than lactase on calcium alginate at the optimum temperature 50 ºC. Lactase immobilized on calcium alginate and magnetic chitosan showed significantly different enzymatic activity, stability, and reusability.

Nickel (II) and Cobalt (II) Alginate Biopolymers as a “Carry and Release” Platform for Polyhistidine-Tagged Proteins

“…Immobilization of proteins using biopolymers as a scaffold often requires normal covalent binding; thus, modifying particular aminoacids residues that might affect stability or biological activity [ 9 , 10 , 11 , 12 ]. Moreover, normal covalent binding is usually targeted towards more than one residue per protein molecule generating multiple possible orientation that can affect substrate availability (enzymes) or epitope recognition by antibodies or T-cell receptors (TCR) [ 13 ].…”