Enzyme‐Amplified Amperometric Detection of DNA Using Redox Mediating Films on Gold Microelectrodes

Hajdukiewicz, Joanna; Boland, Susan; Kavanagh, Paul; Nowicka, Anna M.; Stojek, Zbigniew; Leech, Dónal

doi:10.1002/elan.200804395

Cited by 14 publications

(9 citation statements)

References 54 publications

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“…Then a 6-l droplet containing the PEG cross-linker (2 l of a 15 mg/ml aqueous solution), amino-probe DNA (2 l of a 400 g/ml aqueous solution) and 2 l of water were placed onto the electrode surface to form the sensing layer. Next, the electrode was equilibrated in a dessicator for at least 48 h. From our earlier studies we know that the above ratio of the components guarantees the formation of the best sensing layers (Hajdukiewicz et al, 2009;Kavanagh and Leech, 2006).…”

Section: Probe Immobilization (2)mentioning

confidence: 99%

Construction of DNA biosensor at glassy carbon surface modified with 4-aminoethylbenzenediazonium salt

Kowalczyk

Nowicka

Jurczakowski

et al. 2011

Biosensors and Bioelectronics

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Section: Probe Immobilization (2)mentioning

confidence: 99%

Construction of DNA biosensor at glassy carbon surface modified with 4-aminoethylbenzenediazonium salt

Kowalczyk

Nowicka

Jurczakowski

et al. 2011

Biosensors and Bioelectronics

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“…Schemes of the entire procedures for the preparation of the sensors using three ways of immobilization of probe DNA and the names of biosensors are given in Figure 1. The details for the construction of the particular biosensors are given in the references provided: electrochemical intercalative probe‐labeled DNA biosensor 29, 30, electrochemical enzyme–labeled DNA biosensor 31, 32 and gravimetric DNA biosensor 33, 34…”

Section: Resultsmentioning

confidence: 99%

Substantial Influence of Temperature on Anchoring of Gold‐Nanoparticle Monolayer for Performance of DNA Biosensors

et al. 2010

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This paper presents a way of modification of crystalline gold surface with a high quality layer of gold nanoparticles (Au NPs) via self-assembled dithiol. The application of additional Au NPs monolayer prepared at various temperatures was tested with three types of biosensors previously described in the literature. The examined DNA biosensors differed by the detection method and the way of the immobilization of DNA probe at the modified gold electrode surface. For the immobilization of DNA probe in the sensing layer either the formation of SAM or the affinity binding (biotin -sterptavidin) or covalent attachment were used. The necessary condition of successful preparation of a perfect such monolayer is the preparation temperature of 4 8C. The preparation of Au NPs layers at higher than 4 8C temperatures leads to poor repeatability and unsatisfactory precision of the measurements. The application of the perfect Au monolayer lowers the detection limit (circa by 10 to 100 times) for all tested DNA biosensors.

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“…This sensor does not require labeling of the target biomolecules; rather, only the reporting molecules are labeled (56–58). The biorecognition process is carried out by monitoring changes in the amount or EC properties of the reporter molecules.…”

Section: Electrochemical Dna Sensor Typesmentioning

confidence: 99%

DNA Diagnostics: Nanotechnology-Enhanced Electrochemical Detection of Nucleic Acids

2010

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ABSTRACT:The detection of mismatched base pairs in DNA plays a crucial role in the diagnosis of genetic-related diseases and conditions, especially for early stage treatment. Among the various biosensors that have been used for DNA detection, EC sensors show great promise because they are capable of precise DNA recognition and efficient signal transduction. Advancements in micro-and nanotechnologies, specifically fabrication techniques and new nanomaterials, have enabled for the development of highly sensitive, highly specific sensors making them attractive for the detection of small sequence variations. Furthermore, the integration of sensors with sample preparation and fluidic processes enables for rapid, multiplexed DNA detection essential for POC clinical diagnostics. T he recent discovery and sequencing of the human genome has provided valuable insight into understanding how genetic factors contribute to the development of disease. Specifically, the detection of DNA sequence variations plays an important role in the diagnosis of genetic-related diseases and conditions, especially for early stage treatment and monitoring. Among the different types of diseases caused by DNA alterations, sequence-specific mismatch has the most importance, yet is extremely difficult to detect (1), especially for single-nucleotide polymorphism (SNP). Furthermore, sequence-specific detection has great importance in various medical and scientific applications such as the diagnosis of inherited diseases and the study of pathogen response and bacterial/viral detection.Because of the complex nature of DNA, the detection of single or small numbers of base mismatches requires high sensitivity and specificity (2-4). Current detection methods rely on sample amplification combined with meticulous experimental stringency control (5). For example, polymerase chain reaction (PCR) requires careful primer design and accurate temperature control to obtain sensitivities in the fM range with single-base mismatch specificity (1,3,4). Although these conventional technologies provide the golden standard for laboratory-based DNA diagnostics, they cannot meet the requirements of POC clinical diagnostics (4).EC sensors, initially developed to detect biomolecules in a laboratory setting, have recently found extensive applications for on-site biosensing and detection (6,7), especially for medical and clinical diagnostics (8 -12). While offering simplicity in operation and sample manipulation, the contemporary EC biosensor also provides highly sensitive and specific measurements for a broad spectrum of biomolecules (13-17). The sample size required for current EC sensors is small, ranging from several microliters to hundreds of nanoliters, which includes the sample pretreatment reagents. Additionally, the detection time is relatively fast, varying from a few minutes to tens of seconds. However, the most important feature of EC sensors is their potential to be easily transformed from a laboratory-based instrument to a commercializable POC device. Because o...

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Enzyme‐Amplified Amperometric Detection of DNA Using Redox Mediating Films on Gold Microelectrodes

Cited by 14 publications

References 54 publications

Construction of DNA biosensor at glassy carbon surface modified with 4-aminoethylbenzenediazonium salt

Construction of DNA biosensor at glassy carbon surface modified with 4-aminoethylbenzenediazonium salt

Substantial Influence of Temperature on Anchoring of Gold‐Nanoparticle Monolayer for Performance of DNA Biosensors

DNA Diagnostics: Nanotechnology-Enhanced Electrochemical Detection of Nucleic Acids

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