Enzyme-Assisted Extraction of Bioactives

Marathe, Sandesh J.; Jadhav, Swati B.; Bankar, Sandip B.

doi:10.1007/978-3-319-51639-4_8

Cited by 41 publications

(36 citation statements)

References 128 publications

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“…Nevertheless, the present findings confirmed previous reports on the efficient extraction of phenolics from other plant materials by the use of enzymes. 30…”

mentioning

confidence: 99%

Influence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petals

2020

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Peony is a less utilized herbaceous plant of ornamental and medicinal relevance. The petals are rich in bioactive phenolics and hence, the development of efficient extraction is required for maximum recovery and bioactivity. In this study, the optimal conditions for the ultrasound-assisted extraction of peony phenolics were first investigated by comparing to conventional extraction and testing the data fit by mathematical models (linear, two factor interactions, quadratic) followed by investigation of cellulase pre-treatment combined with ultrasonication. High amounts of flavonoids, phenolics and tannins were extracted in 30 min under ultrasound conditions of 150 W, 40 kHz, 50 % amplitude, in 70 % ethanol with 50/1 solvent/solid ratio. A further cellulase pre-treatment at 40 °C for different incubation times combined with ultrasonication facilitated the extraction of anthocyanins (720.48 mg/100 g DW), phenolics (3985.50 mg/100 g DW), flavonoids (2369.28 mg/100 g DW) and tannins (8917.81 mg/100 g DW). Good antioxidant activities by FRAP (350.03 mg ascorbic acid/100 g DW) and DPPH (inhibition values >80 %) were registered showing great potential for developing high-value bioingredients. The FTIR analysis revealed the presence of characteristic functional groups. The hereby-combined extraction methods could be extended to other peony species.

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“…Nevertheless, the present findings confirmed previous reports on the efficient extraction of phenolics from other plant materials by the use of enzymes. 30…”

mentioning

confidence: 99%

Influence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petals

2020

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“…The plausible explanation for this was that thermal degradation would occur and enzymes might be deactivated. [26,27] Therefore, this study was in accordance with previous research and three levels encompassing 50, 55, and 60°C of incubation temperature were selected for RSM experiments with the central point at 55°C.…”

Section: Effect Of Incubation Temperaturementioning

confidence: 96%

Integrating of Aqueous Enzymolysis with Ethanolic Ultrasonication to Ameliorate Cordycepin Content from Vietnamese Cordyceps militaris and Biological Analysis of Extracts

Thanh

Dat

et al. 2021

ChemistrySelect

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In this study, the employment of aqueous enzymolysis using alcalase with ethanolic ultrasonication extraction method was investigated. The cordycepin content was analyzed by using reversed‐phase high‐performance liquid chromatography with a diode array detector. The result showed that the highest cordycepin content of 0.4365±0.0309 mg/g could be obtained under extraction conditions including total enzyme volume of 240 μL, incubation time of 50 min, incubation temperature of 55 °C, and ultrasonic power of 840 W. The comparative studies of antioxidant activities of the extract were determined in terms of the DPPH free radical scavenging assay, ferric ion reducing power assay, and H2O2 scavenging assay. Additionally, the MTT assay was conducted to evaluate the anticancer activities of the extract, illustrating the cytotoxic property against Hep‐G2 cancer cells with the half‐maximal inhibitory concentration (IC50) value of 12.20±0.03 μg/mL compared to that of 0.36±0.03 μg/mL for ellipticine, MCF‐7 cancer cells with the IC50 value of 11.60±0.09 μg/mL compared to that of 0.51±0.04 μg/mL for ellipticine, and Lu cancer cells with the IC50 value of 34.50±0.25 μg/mL compared to that of 0.32±0.03 μg/mL for ellipticine.

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“…In this case, the carbohydrases would break the cell wall components, leading to the release of oil. At the same time, the oil droplets that are embedded in fibrous protein would be hydrolyzed by the proteases, thus improving the overall yield [11]. It should be noted that while the proteolytic enzymes could facilitate the release of oil, the concurrent breakdown of protein could also lead to an increase in its emulsifying capacity, which could lower the extraction of free oil.…”

Section: Mechanism Of Enzymatic Extraction Of Bioactivesmentioning

confidence: 99%

Improvements in the extraction of bioactive compounds by enzymes

Marathe

Jadhav

Bankar

et al. 2019

Current Opinion in Food Science

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Bioactives from plants are always in high demand in nutraceutical, pharmaceutical and functional food sectors due to their health benefits. This intensifies the need of extraction of bioactives by different methods that can improve the yield and purity of the compound. Enzyme helps in the release of bioactives from the plant material under optimized conditions so as to make the extraction process efficient. Though enzymatic extraction of bioactive has been used since long, it needs improvement to further enhance the yield, reduce the process time and to make the process cost competitive. The combination of enzymatic extraction with other green techniques such as ultrasound extraction, supercritical fluid extraction, three phase partitioning, ionic liquid extraction and microwave extraction can boost the advantages of enzymatic extraction. This review focuses on the improvement in the enzymatic extraction techniques for the bioactive in detail. Keywords Bioactives Enzyme-assisted extraction (EAE) Ultrasound assisted enzyme extraction (UAEE) Enzyme-assisted supercritical extraction (EASCFE) Ionic liquids enzyme assisted extraction (ILEAE) Microwave assisted enzymatic extraction (MAEE) Enzyme assisted three phase partition (EATPP) High pressure assisted enzymatic extraction (HPAEE)

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Enzyme-Assisted Extraction of Bioactives

Cited by 41 publications

References 128 publications

Influence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petals

Influence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petals

Integrating of Aqueous Enzymolysis with Ethanolic Ultrasonication to Ameliorate Cordycepin Content from Vietnamese Cordyceps militaris and Biological Analysis of Extracts

Improvements in the extraction of bioactive compounds by enzymes

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