Enzyme-assisted extraction of fucoxanthin and lipids containing polyunsaturated fatty acids from Undaria pinnatifida using dimethyl ether and ethanol

Billakanti, Jagan; Catchpole, Owen J.; Fenton, Tina; Mitchell, Kevin; MacKenzie, Andrew

doi:10.1016/j.procbio.2013.09.015

Cited by 98 publications

(57 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Broad ranges have been reported for all three of these parameters, using various protocols. Grosso et al (2015) reviewed extraction methods ranging from pH 3.8 to 8.5; Bidigare et al (2005) reported using 0°C for up to 24 h, Billakanti et al (2013) 37°C for 2 h at pH 6.2, Quitain et al (2013) 40°C for 3 h, Sivagnanam et al (2015) 45°C for 2 h, Roh et al (2008) 49.85°C for 50 min, and Shang et al (2011) pigment-containing thylakoids in the blades, which function as the photosynthesis engines of macroalgae, compared to the stipe or holdfasts which have structural functions. Photoprotection of thallus regions near the ocean surface against ultraviolet light is another function of fucoxanthin in the blades, where it exerts its powerful antioxidant effect (Lobban and Harrison 1994;Stengel and Dring 1998;Hurd et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…It is readily soluble in acetone, and absorbs light at wavelengths in the same range as fucoxanthin. It has been reported to have a retention time greater than fucoxanthin, and therefore elute slightly later (Bidigare et al 2005;Billakanti et al 2013). Purification of Peak D in Fig.…”

Section: Hplc Separation Of Fucoxanthinmentioning

confidence: 93%

“…HPLC-DAD analysis of fucoxanthin Chromatographic analysis of seaweed extracts was carried out according to a modified method developed by Billakanti et al (2013). Fucoxanthin separation was achieved with HPLC (Alliance-Waters e2695 Separations Module, 400 atm pressure, at 4°C), equipped with a C 18 reverse phase column (Waters XSelect, 4.6 mm × 100 mm, 3.5 μm particle size), and a UV photodiode array detector (Waters 2998).…”

Section: Quantification Of Fucoxanthinmentioning

confidence: 99%

See 2 more Smart Citations

Optimisation of fucoxanthin extraction from Irish seaweeds by response surface methodology

Shannon¹,

Abu‐Ghannam²

2016

J Appl Phycol

View full text Add to dashboard Cite

Fucoxanthin is a xanthophyll pigment which occurs in marine brown algae (Phaeophyceae). The anti-diabetic, anti-obesity, anti-cancer, and antioxidant properties of fucoxanthin have been widely reported. Macroalgae, particularly brown seaweeds, grow prolifically around Irish coasts, representing a valuable resource of nutraceuticals such as fucoxanthin for functional food applications. The aim of this study was to maximise the solvent extraction yield from three anatomically discrete regions of the seaweed thallus: blade, stipe, and holdfast. Response surface methodology was applied to determine optimum parameters for extraction of fucoxanthin from the seaweed, Fucus vesiculosus, as a model species. A central composite design was applied with four extraction variables: time (30-70 min), temperature (30-70°C), solvent pH (5.0-9.0), and percentage acetone (30-70 %). Fucoxanthin content of extracts was quantified by highperformance liquid chromatography. Percentage acetone was found to have the most significant (P = 0.0002) effect on fucoxanthin yield, followed by pH (P = 0.028) and temperature (P = 0.049). Multiple response optimisation determined that fucoxanthin yield from F. vesiculosus may be maximised by incubating at 30.0°C for 36.5 min, pH 5.7, with 62.2 % acetone. Optimised responses were applied to a further nine brown seaweeds; Alaria esculenta, Ascophyllum nodosum, Fucus serratus, Himanthalia elongata, Laminaria digitata, Laminaria hyperborea, Pelvetia canaliculata, Saccharina latissima, and Saccorhiza polyschides. In all species, the blades contained significantly more fucoxanthin than stipes, while holdfasts contained the least. Alaria esculenta blade had the greatest yield (0.870 mg g −1 dry mass), followed by ).

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Hplc Separation Of Fucoxanthinmentioning

confidence: 93%

Section: Quantification Of Fucoxanthinmentioning

confidence: 99%

See 1 more Smart Citation

Optimisation of fucoxanthin extraction from Irish seaweeds by response surface methodology

Shannon¹,

Abu‐Ghannam²

2016

J Appl Phycol

View full text Add to dashboard Cite

show abstract

“…Another consideration for fucoxanthin as a functional food is the high cost, due to the energy required for the extraction and freeze-drying process (Billakanti et al 2013) and the lack of an artificial synthesis method for fucoxanthin. Seasonal variations in fucoxanthin nutritional content (Fung et al 2013) could also affect health claims and nutritional efficacy.…”

Section: Fucoxanthin As a Functional Food: Challenges And Opportunitiesmentioning

confidence: 99%

“…Billakanti et al (2013) reported a significant increase (9.3%) in fucoxanthin extraction yields from wakame (Undaria pinnatifida) using alginate lyase derived from Flavobacterium multivorum as a pre-processing step, followed by dimethyl ether and ethanol extraction, compared to untreated preprocessing. Optimum enzyme pre-treatment parameters were found to be 37 °C, for 2 hours, at pH 6.2, 5% (w/v) solids, with 0.05 wt% enzyme using …”

Section: Enzymatic Extractionmentioning

confidence: 99%