Enzyme‐Assisted Hydrogel Formation for Tissue Engineering Applications

Pérez‐Rafael, Sílvia; Ramon, Eva; Tzanov, Tzanko

doi:10.1002/9783527825820.ch4

Cited by 2 publications

(1 citation statement)

References 178 publications

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“…These limitations generated by crosslinking methods can be overcome by using enzymes to form covalently crosslinked hydrogels. Enzyme-mediated crosslinking by tyrosinases [ 16 ], transferases [ 17 ] or peroxidases [ 18 ] have proven their efficiency and therefore, they have attracted increasing attention for application in polymer hydrogel synthesis due to the environmentally-friendly process and the possibility of obtaining biomaterials with extracellular matrix-mimicking properties. Transglutaminase (TGase), a widely present enzyme in nature, has been demonstrated to catalyze the formation of isopeptide bonds between proteins by mimicking in vivo biosynthetic processes, which can significantly improve protein gelation [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Enzymatically-Crosslinked Gelatin Hydrogels with Nanostructured Architecture and Self-Healing Performance for Potential Use as Wound Dressings

Rusu

Niţă

Simiónescu³

et al. 2023

Polymers

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Development of natural protein-based hydrogels with self-healing performance and tunable physical properties has attracted increased attention owing to their wide potential not only in the pharmaceutical field, but also in wounds management. This work reports the development of a versatile hydrogel based on enzymatically-crosslinked gelatin and nanogels loaded with amoxicillin (Amox), an antibiotic used in wound infections. The transglutaminase (TGase)-crosslinked hydrogels and encapsulating nanogels were formed rapidly through enzymatic crosslinking and self-assembly interactions in mild conditions. The nanogels formed through the self-assemble of maleoyl-chitosan (MAC5) and polyaspartic acid (PAS) may have positive influence on the self-healing capacity and drug distribution within the hydrogel network through the interactions established between gelatin and gel-like nanocarriers. The physicochemical properties of the enzymatically-crosslinked hydrogels, such as internal structure, swelling and degradation behavior, were studied. In addition, the Amox release studies indicated a rapid release when the pH of the medium decreased, which represents a favorable characteristic for use in the healing of infected wounds. It was further observed through the in vitro and in vivo biocompatibility assays that the optimized scaffolds have great potential to be used as wound dressings.

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Section: Introductionmentioning

confidence: 99%

Enzymatically-Crosslinked Gelatin Hydrogels with Nanostructured Architecture and Self-Healing Performance for Potential Use as Wound Dressings

Rusu

Niţă

Simiónescu³

et al. 2023

Polymers

View full text Add to dashboard Cite

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Injectable Smart Nanocomposite Hydrogels for Biomedical Applications

Zeinali,

Tzanov

2024

Injectable Smart Hydrogels for Biomedical Applications

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Due to numerous beneficial features, injectable smart NC hydrogels are attractive alternatives for many biomedical applications. Their soft hydrated three-dimensional (3D) porous structure and excellent biocompatibility account for the broad application of these biomaterials for the delivery of cells, signaling and bioactive molecules, and also pharmaceuticals. Moreover, injectable hydrogels provide minimally-invasive delivery to the patient’s body and their stimuli-responsiveness allows for on-demand and site-specific release of the active load with controllable kinetics. Nanomaterials as both structural and functional elements in the hydrogels endow them with enhanced properties and advanced functionalities, improving their efficacy for specific biomedical uses. Tissue engineering and regenerative medicine, drug delivery systems, cancer therapy and wound healing are the most frequently explored and reported applications of this class of biomaterials in the biomedical sector that have been specifically addressed in this chapter.

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Enzyme‐Assisted Hydrogel Formation for Tissue Engineering Applications

Cited by 2 publications

References 178 publications

Enzymatically-Crosslinked Gelatin Hydrogels with Nanostructured Architecture and Self-Healing Performance for Potential Use as Wound Dressings

Enzymatically-Crosslinked Gelatin Hydrogels with Nanostructured Architecture and Self-Healing Performance for Potential Use as Wound Dressings

Injectable Smart Nanocomposite Hydrogels for Biomedical Applications

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