Enzyme-assisted isothermal amplification of nucleic acids on the electrode surface

Cobzariu, Madalin Alexandru; Lobo-Castañón, Marı́a Jesús; Miranda‐Castro, Rebeca

doi:10.1016/j.coelec.2023.101322

Current Opinion in Electrochemistry

2023

DOI: 10.1016/j.coelec.2023.101322

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Enzyme-assisted isothermal amplification of nucleic acids on the electrode surface

Madalin Alexandru Cobzariu

Marı́a Jesús Lobo-Castañón

Rebeca Miranda‐Castro

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Cited by 2 publications

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“…Moreover, CRISPR/Cas technologies have been successfully implemented on paper, integrated into microfluidic devices, 123 and coupled to other amplification strategies. 124 In addition, the progress made in coupling electrochemical biosensing with isothermal nucleic acid amplification strategies, specifically helicase-dependent amplification (HDA), loop- mediated isothermal amplification (LAMP), and recombinase polymerase amplification (RPA), which have been successfully implemented even on the electrode surface, 15,116,125 is considered very important to bring these new platforms to the real practice as PON tests.…”

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Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

Campuzano

Pingarrón

2023

ACS Sens.

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Electrochemical affinity biosensors are evolving at breakneck speed, strengthening and colonizing more and more niches and drawing unimaginable roadmaps that increasingly make them protagonists of our daily lives. They achieve this by combining their intrinsic attributes with those acquired by leveraging the significant advances that occurred in (nano)materials technology, bio(nano)materials and nature-inspired receptors, gene editing and amplification technologies, and signal detection and processing techniques. The aim of this Perspective is to provide, with the support of recent representative and illustrative literature, an updated and critical view of the repertoire of opportunities, innovations, and applications offered by electrochemical affinity biosensors fueled by the key alliances indicated. In addition, the imminent challenges that these biodevices must face and the new directions in which they are envisioned as key players are discussed.

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confidence: 99%

Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

Campuzano

Pingarrón

2023

ACS Sens.

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Point‐Of‐Care Ultra‐Portable Single‐Molecule Bioassays for One‐Health

Macchia,

Torricelli,

Caputo

et al. 2023

Advanced Materials

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Screening asymptomatic organisms (humans, animals, plants) with a high‐diagnostic accuracy using point‐of‐care‐testing (POCT) technologies, though still visionary holds great potential. Convenient surveillance requires easy‐to‐use, cost‐effective, ultra‐portable but highly reliable, in‐vitro‐diagnostic devices that are ready for use wherever they are needed. Currently, there are not yet such devices available on the market, but there are a couple more promising technologies developed at readiness‐level 5: the Clustered‐Regularly‐Interspaced‐Short‐Palindromic‐Repeats (CRISPR) lateral‐flow‐strip tests and the Single‐Molecule‐with‐a‐large‐Transistor (SiMoT) bioelectronic palmar devices. They both hold key features delineated by the World‐Health‐Organization for POCT systems and an occurrence of false‐positive and false‐negative errors <1–5% resulting in diagnostic‐selectivity and sensitivity >95–99%, while limit‐of‐detections are of few markers. CRISPR‐strip is a molecular assay that, can detect down to few copies of DNA/RNA markers in blood while SiMoT immunometric and molecular test can detect down to a single oligonucleotide, protein marker, or pathogens in 0.1mL of blood, saliva, and olive‐sap. These technologies can prospectively enable the systematic and reliable surveillance of asymptomatic ones prior to worsening/proliferation of illnesses allowing for timely diagnosis and swift prognosis. This could establish a proactive healthcare ecosystem that results in effective treatments for all living organisms generating diffuse and well‐being at efficient costs.

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Enzyme-assisted isothermal amplification of nucleic acids on the electrode surface

Cited by 2 publications

References 52 publications

Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

Point‐Of‐Care Ultra‐Portable Single‐Molecule Bioassays for One‐Health

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