Enzyme-assisted modification of flavonoids from Matricaria chamomilla: antioxidant activity and inhibitory effect on digestive enzymes

Abstract

Matricaria chamomilla L. contains antioxidant flavonoids that can have their bioactivity enhanced by enzymatic hydrolysis of specific glycosyl groups. This study implements an untargeted metabolomics approach based on ultra-performance liquid chromatography coupled with electrospray ionisation quadrupole time-of-flight mass spectrometry technique operating in MSE mode (UPLC-QTOF-MSE) and spectrophotometric analysis of chamomile aqueous infusions, before and after hydrolysis by hesperidinase and β-galactosidase. Several phenolic compounds were altered in the enzymatically treated infusion, with the majority being flavonoid derivatives of apigenin, esculetin, and quercetin. Although enzymatically modifying the infusion only led to a small increase in antioxidant activity (DPPH• method), its inhibitory effect on pancreatic lipase was of particular interest. The enzymatically treated infusion exhibited a greater inhibitory effect (EC50 of 35.6 µM) than unmodified infusion and kinetic analysis suggested mixed inhibition of pancreatic lipase. These results are of great relevance due to the potential of enzymatically treated functional foods in human health.

In vitro antioxidant and antidiabetic activity of essential oils encapsulated in gelatin‐pectin particles against sugar, lipid and protein oxidation and amylase and glucosidase activity

“…Among the phytochemicals that have been studied, phenolic compounds (Kwon, Apostolidis, & Shetty, 2008) and flavonoids (Majeed et al., 2020) have demonstrated the highest inhibitory effects against amylases. Phenolic compounds with noncompetitive or uncompetitive inhibition strategies prevent amylase activity (Franco et al., 2020).…”

Role of Annexin A1 in NLRP3 Inflammasome Activation in Murine Neutrophils

“…HPLC-grade acetonitrile (ACN) and isopropanol were from Honeywell (Morristown, NJ, USA). Data were acquired using an ACQUITY FTN liquid chromatograph coupled to a XEVO-G2XSQTOF mass spectrometer (Waters, Milford, MA, USA) using MassLynx 4.1 software as previously described [ 19 ]. Briefly, a Supelcosil LC-18 (2.1 × 100 mm 2 , 5.0 µm, Supelco) column was used.…”

Advanced Analytical Approaches for the Analysis of Polyphenols in Plants Matrices—A Review

“…The enzymatic activity of cellulases, pectinases and hemicellulases leads to cell wall disruption and enhances the extraction of valuable compounds from plants [43]. Furthermore, due to the enzymatic activity of lyases and hydrolases on the glycosidic fractions of natural polyphenols, their biological properties are improved in terms of bioactivity and bioavailability [44]. A mixture of hesperidinase and β-galactosidase was used in a very recent study for the extraction of flavonoids from Matricaria chamomilla [44].…”

“…Furthermore, due to the enzymatic activity of lyases and hydrolases on the glycosidic fractions of natural polyphenols, their biological properties are improved in terms of bioactivity and bioavailability [44]. A mixture of hesperidinase and β-galactosidase was used in a very recent study for the extraction of flavonoids from Matricaria chamomilla [44]. Flavonoids, as well as non-flavonoid polyphenols, were significantly structurally modified by the enzymatic treatment, which resulted in the increased bioactivity of the metabolites as inhibitors of pancreatic lipase activity [44].…”