Enzyme-assisted optimized supercritical fluid extraction to improve Medicago sativa polyphenolics isolation

Krakowska, Aneta; Rafińska, Katarzyna; Walczak, Justyna; Buszewski, Bogusław

doi:10.1016/j.indcrop.2018.08.004

Cited by 50 publications

(34 citation statements)

References 33 publications

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“…Optimal extraction parameters for total polyphenol content were: 68 • C, 205 bar, and 15.5% for temperature, pressure and co-solvent content, respectively. This methodology provides effective enzyme-assisted supercritical fluid extraction for the enhanced release of polyphenol compounds [129]. Supercritical fluids are a more efficient, safer, and environmentally friendly method for extracting and recovering flavonoids for the purpose of bioactive compounds study (Table 2C).…”

Section: Supercritical Fluid Extractionmentioning

confidence: 99%

Conventional and Emerging Extraction Processes of Flavonoids

et al. 2020

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Flavonoids are a group of plant constituents called phenolic compounds and correspond to the nonenergy part of the human diet. Flavonoids are found in vegetables, seeds, fruits, and beverages such as wine and beer. Over 7000 flavonoids have been identified and they have been considered substances with a beneficial action on human health, particularly of multiple positive effects because of their antioxidant and free radical scavenging action. Although several studies indicate that some flavonoids have provident actions, they occur only at high doses, confirming in most investigations the existence of anti-inflammatory effects, antiviral or anti-allergic, and their protective role against cardiovascular disease, cancer, and various pathologies. Flavonoids are generally removed by chemical methods using solvents and traditional processes, which besides being expensive, involve long periods of time and affect the bioactivity of such compounds. Recently, efforts to develop biotechnological strategies to reduce or eliminate the use of toxic solvents have been reported, reducing processing time and maintaining the bioactivity of the compounds. In this paper, we review, analyze, and discuss methodologies for biotechnological recovery/extraction of flavonoids from agro-industrial residues, describing the advances and challenges in the topic.

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Section: Supercritical Fluid Extractionmentioning

confidence: 99%

Conventional and Emerging Extraction Processes of Flavonoids

et al. 2020

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“…It is obvious from Figure 4c,d and Figure 4g,h that after the enzymatic hydrolysis, the plant materials are wrinkled and highly porous in comparison with control where is observed the smooth surface of plant particles (Figure 4a,b and Figure 4e,f). Overall, visible morphological changes indicate that the use of enzymatic hydrolysis before the extraction causes an increase in surface area which improves mass transfer rate and CO2 and cosolvent distribution and finally results in higher liberation efficiency of polyphenolic compounds [13,18]. Overall, visible morphological changes indicate that the use of enzymatic hydrolysis before the extraction causes an increase in surface area which improves mass transfer rate and CO 2 and co-solvent distribution and finally results in higher liberation efficiency of polyphenolic compounds [13,18].…”

Section: Effect Of Enzymatic Hydrolysismentioning

confidence: 99%

“…The effectiveness of enzymatic processes depends largely on the type of enzyme or enzyme mixture used and the selection of optimal conditions for their operation (pH, ionic strength, temperature). Therefore, the development of a new effective and selective protocols for increased recovery of bioactive substances, especially from plants in which the cell wall may be a physical barrier preventing effective extraction would be beneficial [9][10][11][12][13]. To date, there is little information about extraction of polyphenolic compounds by supercritical fluid extraction supported by enzymes from yerba mate and yellow lupine−two important species from a pharmaceutical and supplement industry point of view.…”

Section: Introductionmentioning

confidence: 99%

The Influence of Plant Material Enzymatic Hydrolysis and Extraction Conditions on the Polyphenolic Profiles and Antioxidant Activity of Extracts: A Green and Efficient Approach

et al. 2020

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The aim of this study was to develop a new comprehensive extraction protocol based on green technology for the enhanced release of polyphenolic compounds from plant cells. In this work, extracts from yerba mate and yellow lupine seed were obtained by using three different extraction techniques: maceration, supercritical fluid extraction with co-solvent (SFE) and enzyme assisted-supercritical fluid extraction with co-solvent (EA-SFE). Several experimental parameters such as time, type of solvent and co-solvent as well as CO2 flow rate were selected to obtain the highest extraction efficiency. The chemical profiles in the obtained extracts and their biological activity were evaluated. HPLC-MS/MS analysis indicated that the level of phenolic compounds in extracts from yerba mate obtained by EA-SFE was approximately five times higher than for maceration and 3.2 times higher than for SFE. In the case of extracts from yellow lupine seed an approximately 5.6-fold increase was observed in comparison with maceration and SFE with 96% MeOH, and 2.9 times for SFE with 96% EtOH. The developed protocol with a mix of enzymes commonly applied in the agricultural industry significantly raises the efficiency of liberation of secondary metabolites.

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“…The resulted powder was passed through a sieve with mesh size 1 mm. Consequently, particles with the dimension equal of less than 1 mm were used (Krakowska et al 2018). For each plant included in the study, 1 g of grounded powder was placed in a 10 mL stainless steel extraction cell and extracted using water as a solvent.…”

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confidence: 99%

Simultaneous Determination of Cyclitols and Sugars Following a Comprehensive Investigation of 40 Plants

et al. 2019

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Due to the important features of widely unexplored cyclitols, a comprehensive qualitative and quantitative study is needed. Moreover, measuring the possible available amounts of identified components in plant material represents a stringent need, due to their importance in phytomedicine and their use in food. The purpose of this study was to realize an extended investigation mainly of cyclitols, but of sugars and sugar alcohols as well, from natural sources. Thus, 17 target compounds (7 cyclitols and 2 sugar alcohols and 8 sugars) extracted from medicinal and edible plants are reported. All detected components were simultaneous separated in just one chromatographic run, using a single GC column. A number of 52 sources coming from 40 species were studied. Thus, we report 37 new sources of cyclitols. Moreover, almost for all cyclitols, the richest source was not investigated previously. Therefore, the obtained results can represent a valuable material for food, pharmaceutical, medical, or cosmetic industry interested in the use of cyclitols.

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Enzyme-assisted optimized supercritical fluid extraction to improve Medicago sativa polyphenolics isolation

Cited by 50 publications

References 33 publications

Conventional and Emerging Extraction Processes of Flavonoids

Conventional and Emerging Extraction Processes of Flavonoids

The Influence of Plant Material Enzymatic Hydrolysis and Extraction Conditions on the Polyphenolic Profiles and Antioxidant Activity of Extracts: A Green and Efficient Approach

Simultaneous Determination of Cyclitols and Sugars Following a Comprehensive Investigation of 40 Plants

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