Enzyme assisted peptide self-assemblies trigger cell adhesion in high density oxime based host gels

Criado‐Gonzalez, Miryam; Loftin, Breyinn; Fores, Jennifer Rodon; Vautier, Dominique; Kocgozlu, Leyla; Jierry, Loı̈c; Schaaf, Pierre; Boulmedais, Fouzia; Harth, Eva

doi:10.1039/d0tb00456a

Cited by 18 publications

(9 citation statements)

References 42 publications

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“…Besides the introduction of gelatin or collagen in the ink [ 16 ], one strategy is to graft different cell adhesive peptides to alginate [ 34 , 35 ]. Recently, Criado-Gonzalez and coworkers [ 36 ] showed that the incorporation of a self-assembled phosphorylated tri-peptide, Fmoc-FFY (Fmoc: 9-fluorenylmethoxycarbonyl; F: phenylalanine; Y: tyrosine), within the polyethylene glycol [ 37 ] or hyaluronic acid hydrogels allowed for the promotion of fibroblast cell adhesion [ 36 , 38 ]. Moreover, this peptide exhibited antibacterial and anti-inflammatory properties [ 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of the Rheological Properties of Self-Assembled Tripeptide/Alginate/Cellulose Hydrogels for 3D Printing

et al. 2022

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3D printing is an emerging and powerful technique to create shape-defined three-dimensional structures for tissue engineering applications. Herein, different alginate–cellulose formulations were optimized to be used as printable inks. Alginate (Alg) was chosen as the main component of the scaffold due to its tunable mechanical properties, rapid gelation, and non-toxicity, whereas microcrystalline cellulose (MCC) was added to the hydrogel to modulate its mechanical properties for printing. Additionally, Fmoc-FFY (Fmoc: 9-fluorenylmethoxycarbonyl; F: phenylalanine; Y: tyrosine), a self-assembled peptide that promotes cell adhesion was incorporated into the ink without modifying its rheological properties and shear-thinning behavior. Then, 3D-printed scaffolds made of Alg, 40% of MCC inks and Fmoc-FFY peptide were characterized by scanning electron microscopy and infrared spectroscopy, confirming the morphological microstructure of the hydrogel scaffolds with edged particles of MCC homogeneously distributed within the alginate matrix and the self-assembly of the peptide in a β-sheet conformation. Finally, the cytocompatibility of the scaffolds was tested in contact with the MG63 osteosarcoma cells, confirming the absence of cytotoxic components that may compromise their viability. Interestingly, MG63 cell growth was retarded in the scaffolds containing the peptide, but cells were more likely to promote adhesive interactions with the material rather than with the other cells, indicating the benefits of the peptide in promoting biological functionality to alginate-based biomaterials.

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Section: Introductionmentioning

confidence: 99%

Optimization of the Rheological Properties of Self-Assembled Tripeptide/Alginate/Cellulose Hydrogels for 3D Printing

et al. 2022

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“…In our study, we have investigated the tripeptide Fmoc-FF p Y as precursor and Fmoc-FFY as hydrogelator. This is a model system well studied by our group over the last years [ 8 , 11 , 16 , 17 , 18 ]. When Fmoc-FF p Y (5 mg/mL, 6.44 mM) is dissolved in buffer solution at room temperature (Borax buffer 25 mM, pH 9.5, 20 °C), the addition of AP (1 mg/mL, 6.67 µM) induces the self-assembly of Fmoc-FFY in β-sheet organization and then the supramolecular hydrogel is obtained in 20 min.…”

Section: Resultsmentioning

confidence: 99%

“…This opened the route to the precise localization of peptide self-assemblies, and a step that was taken in 2009 when Williams and Ulijn groups showed that peptide self-assembly can be initiated from covalently immobilized enzyme on a surface [ 10 ]. Our group further developed the localized enzyme-assisted self-assembly (LEASA approach by introducing seed-layers in addition to an enzyme-adsorbed layer to trigger the localized self-assembly growth [ 11 , 12 ], for catalytic and auto-catalytic self-assembled hydrogels [ 13 , 14 , 15 ] or self-assemblies initiated from nanoparticles surface [ 16 ] and in host materials [ 17 , 18 ]. This bottom-up approach is efficient in water and once the surface coated with an adequate enzyme, the localized self-assembled structure growth proceeds in an autonomous way [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Localized Enzyme-Assisted Self-Assembly in the Presence of Hyaluronic Acid for Hybrid Supramolecular Hydrogel Coating

et al. 2021

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Hydrogel coating is highly suitable in biomaterial design. It provides biocompatibility and avoids protein adsorption leading to inflammation and rejection of implants. Moreover, hydrogels can be loaded with biologically active compounds. In this field, hyaluronic acid has been largely studied as an additional component since this polysaccharide is naturally present in extracellular matrix. Strategies to direct hydrogelation processes exclusively from the surface using a fully biocompatible approach are rare. Herein we have applied the concept of localized enzyme-assisted self-assembly to direct supramolecular hydrogels in the presence of HA. Based on electronic and fluorescent confocal microscopy, rheological measurements and cell culture investigations, this work highlights the following aspects: (i) the possibility to control the thickness of peptide-based hydrogels at the micrometer scale (18–41 µm) through the proportion of HA (2, 5 or 10 mg/mL); (ii) the structure of the self-assembled peptide nanofibrous network is affected by the growing amount of HA which induces the collapse of nanofibers leading to large assembled microstructures underpinning the supramolecular hydrogel matrix; (iii) this changing internal architecture induces a decrease of the elastic modulus from 2 to 0.2 kPa when concentration of HA is increasing; (iv) concomitantly, the presence of HA in supramolecular hydrogel coatings is suitable for cell viability and adhesion of NIH 3T3 fibroblasts.

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“…The development of stimuli-responsive materials that can be employed as therapeutic agents attracts great attention in the biomedical field for drug delivery, 1,2 tissue engineering, 3,4 or biosensing applications. 5–7 These biomaterials aim to take advantage of biological stimuli like pH, 8 temperature, 9 enzyme activity, 10,11 or redox balance 5,12 to produce specific therapeutic effects. In this regard, reactive oxygen species (ROS), which is the term given to biologically relevant oxidants present in the human body as by-products of aerobic respiration, including hydrogen peroxide (H 2 O 2 ), the superoxide anion (O 2 ˙ − ), hydroxyl radical (˙OH), peroxynitrite (ONOO − ), single oxygen ( 1 O 2 ), and hypochlorite (OCl − ), has gained great importance in the last decade.…”

Section: Introductionmentioning

confidence: 99%

Thioether-based ROS responsive polymers for biomedical applications

Criado‐Gonzalez

Mecerreyes

2022

J. Mater. Chem. B

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Enzyme assisted peptide self-assemblies trigger cell adhesion in high density oxime based host gels

Abstract: Studying the influence of hydrogel matrices on the self-assembly behavior of peptides and interaction with cells guides the future development of engineered biomaterials.

Cited by 18 publications

References 42 publications

Optimization of the Rheological Properties of Self-Assembled Tripeptide/Alginate/Cellulose Hydrogels for 3D Printing

Optimization of the Rheological Properties of Self-Assembled Tripeptide/Alginate/Cellulose Hydrogels for 3D Printing

Localized Enzyme-Assisted Self-Assembly in the Presence of Hyaluronic Acid for Hybrid Supramolecular Hydrogel Coating

Thioether-based ROS responsive polymers for biomedical applications

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