2023
DOI: 10.1002/jsf2.109
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Enzyme‐assisted HPTLC method for the simultaneous analysis of inositol phosphates and phosphate

Abstract: Background The analysis of myo‐inositol phosphates (InsPx) released by phytases during phytic acid degradation is challenging and time‐consuming, particularly in terms of sample preparation, isomer separation, and detection. However, a fast and robust analysis method is crucial when screening for phytases during protein engineering approaches, which result in a large number of samples, to ensure reliable identification of promising novel enzymes or target variants with improved characteristics, for example, pH… Show more

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