Enzyme-catalyzed biodegradation of penicillin fermentation residues by β-lactamase OtLac from Ochrobactrum tritici

Abstract

Background Biodegradation of antibiotics is a promising method for the large-scale removal of antibiotic residues in the environment. However, the enzyme that is involved in the biodegradation process is the key information to be revealed. Results In this study, the beta-lactamase from Ochrobactrumtritici that mediates the biodegradation of penicillin V was identified and characterized. When searching the proteins of Ochrobactrumtritici, the β-lactamase (OtLac) was identified. OtLac consists of 347 amino acids, and predicted isoelectric point is 7.0. It is a class C β-lactamase according to BLAST analysis. The coding gene of OtLac was amplified from the genomic DNA of Ochrobactrumtritici. The OtLac was overexpressed in E. coli BL21 (DE3) and purified with Ni2+ column affinity chromatography. The biodegradation ability of penicillin V by OtLac was identified in an in vitro study and analyzed by HPLC. The optimal temperature for OtLac is 32 ℃ and the optimal pH is 7.0. Steady-state kinetics showed that OtLac was highly active against penicillin V with a Km value of 17.86 μM and a kcat value of 25.28 s−1 respectively. Conclusions OtLac demonstrated biodegradation activity towards penicillin V potassium, indicating that OtLac is expected to degrade penicillin V in the future.

Characterization and Mechanical Properties of Sintered Clay Minerals

“…With increasing antibiotic pollution and consequently avoiding bacteria developing defense mechanisms, the urge for developing methods for successful antibiotic removal are in place (Feng et al 2021;Wang et al 2021;Yang et al 2021). Therefore, any new methods for enhancing enzymatic activity, operational stability and tolerance to pH and temperature of ß-lactamase are being investigated.…”

Waste Rubber Incorporated in the Alkali-Activated Metakaolin’s Aluminosilicate Network Enhanced by Microwave Irradiation

“…As such degradation products can cause allergic reactions as well, some enzymes can catalyze and degrade antibiotics with the advantage of excellent catalytic features, high biocompatibility and eco-friendly performance (Yang et al 2021). Such enzymes are ß-lactamases, which are produced by bacteria that play an important role in their resistance to ß-lactam antibiotics due to their hydrolyzation of ß-lactam ring (Wang et al 2021).…”

Assessment of the efficiency of synergistic photocatalysis on penicillin G biodegradation by whole cell Paracoccus sp

“…In most recently, several research using βlactamase to realize penicillin biodegradation. Although it is exhibited high efficiency, the degradation pathway is relatively simple, and only achieve the hydroxylation of penicillin without further mineralization degradation [44,45]. Our present work was using light irradiation to stimulate the catalytic efficiency of whole cell of KDSPL-02 and also our work realized the mineralization degradation of penicillin.…”