Enzyme-catalyzed C–C bond formation using 2-methyltetrahydrofuran (2-MTHF) as (co)solvent: efficient and bio-based alternative to DMSO and MTBE

Benzaldehyde lyase from Pseudomonas fluorescens Biovar I. (BAL, EC 4.1.2.38) is a versatile catalyst for the organic synthesis of chiral α-hydroxy ketones. To allow fast assessment of enzyme activity, a direct spectrophotometric assay is desirable. Here, a new robust and easy-to-handle assay based on UV absorption is presented. The assay developed is based on the ligation of the α-hydroxy ketone (R)-2,2′-furoin from 2-furaldehyde. A robust assay with direct monitoring of the product is facilitated with a convenient concentration working range minimising experimental associated with low concentrations.

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“…Under these conditions half life of BAL is known to be several hours [10, 13] excluding interference within the assay time span of minutes. …”

Section: Methodsmentioning

confidence: 99%

Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity

Natalia

Kohlmann

Ansorge‐Schumacher

et al. 2011

Biotechnology Research International

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“…2-MeTHF has showed promising results in various types of organic transformations (84), due to its stability in similar conditions as THF and the benefits described above. 2-MeTHF has been used in biocatalysis (85,86), organocatalysis (87), and organometallic transformations (88), and biomass processing (84). It has also been identified as a suitable replacement for dichloromethane (89).…”

Section: -Methyltetrahydrofuranmentioning

confidence: 99%

Opportunities for the Replacement/Minimization of Selective Hazardous Solvents: Applications, Concerns and Approaches To Identify Alternatives

Vaddula

Yalla

Gonzalez

2014

ACS Symposium Series

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“…Beside their promising potential as biofuels, they can also be used as (co-)solvents (Shanmuganathan et al, 2010;Winter, 2010;Geilen et al, 2011;Yang et al, 2014) and EL also as flavor in food (The Good Scents Company, 2015). EL is used as a solvent for cellulose acetate, starch and flavorings (Winter, 2010) and 2-MTHF is a promising candidate to substitute petrochemical solvents in industrial processes (Shanmuganathan et al, 2010;Geilen et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…EL is used as a solvent for cellulose acetate, starch and flavorings (Winter, 2010) and 2-MTHF is a promising candidate to substitute petrochemical solvents in industrial processes (Shanmuganathan et al, 2010;Geilen et al, 2011).…”

Section: Introductionmentioning

confidence: 99%