Enzyme Catalyzed Hydrogel as Versatile Bioadhesive for Tissue Wound Hemostasis, Bonding, and Continuous Repair

Abstract

Developing a versatile bioadhesive which is biocompatible, adhesive, hemostatic, and therapeutic is of great significance to promote wound sealing and healing. Herein, an adhesive (GTT-3 hydrogel) is fabricated by catalysis of tannic acid modified gelatin (Gel-TA) with transglutaminase (TG). The hydrogen bonding, imine linking, and acyl-transfer reaction between GTT-3 hydrogel and tissue enable efficient hydrogel integration and adhesion to tissue instantly, so as to seal the wound and stop bleeding. Moreover, the intrinsic wound healing ability of gelatin and the antibacterial properties of TA provide favorable conditions for wound healing after adhesion. In vitro mechanical property testing and cell experimental results determine the elasticity, adhesion, and biocompatibility of the GTT-3 hydrogel. The wound operation in mouse models and pathological sectioning results indicate that GTT-3 adhesive obviously accelerates hemostasis, wound bonding, and healing. With the special property of instant adhesion and excellent hemostatic and therapeutic repair effects, GTT-3 hydrogel may provide a new option for surgical operation.

Bio-inspired, bio-degradable adenosine 5′-diphosphate-modified hyaluronic acid coordinated hydrophobic undecanal-modified chitosan for hemostasis and wound healing

“…Generally, hydrogel sponges are fabricated by freeze-drying that followed by chemical or physical crosslinking [ 11 ]. Comparably, physical gelation has been developed via hydrogen bonding or electronic interaction [ [12] , [13] , [14] ], which avoids the addition of organic cross-linkers or catalysts, and eliminates unnecessary cytotoxicity functional group introduction in the process of chemical gelation, which displays promising compatibility [ 11 , 15 ]. Furthermore, physical gelation via electronic interaction could be a promising method for the large-scale synthesis of hydrogels just by mixing anionic components with cationic partners.…”

Formulation and Characterization of Hydrophilic Ointment Bases with Carvacrol and Magnolol for Periodontal Application

“…To ensure good stability and maximum efficiency at the application site, the choice of auxiliary substances and preparation technologies is an important step in obtaining appropriate bioadhesive preparations [35]. Hydrogels adhere to the mucous membranes and allow a good release of active substances [36].…”

Transglutaminase-Catalyzed Bottom-Up Synthesis of Polymer Hydrogel

“… Gupta et al (2021) used gelatin hydrogel prepared by MTGase crosslinking as an engineered skeletal muscle matrix, and the hydrogel was stable after 2 weeks under similar culture conditions of natural skeletal muscle. Tannic acid modified gelatin also can be crosslinked by TGase to form hydrogel, and the hydrogel exhibited comparable elasticity and flexibility, and therapeutic healing effects in the mouse skin incision and wound model in vivo ( Zhou et al, 2021 ).…”