Enzyme-Catalyzed Synthesis of Selenium-Doped Manganese Phosphate for Synergistic Therapy of Drug-Resisted Colorectal Tumor

Pei, Manman; Liu, Kaiyuan; Qu, Xiao; Wang, Kairuo; Chen, Qian; Zhang, Yuanyuan; Wang, Xinyue; Wang, Zheng; Li, Xinyao; Chen, Feng; Qin, Huanlong; Zhang, Yang

doi:10.21203/rs.3.rs-1941556/v1

Cited by 1 publication

(1 citation statement)

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“…43,44 High uptake of drugs by cells can produce efficient therapeutic effects. 45 After nanoparticles were wrapped within cancer cell membranes, they gained the ability to bind homologous targets, thereby increasing the uptake of drugs by cancer cells. To detect the cell uptake efficiency of ACM-SSe and RCM-SSe (negative control, A549 cells replaced with red blood cell membranecoated SSe), A549 cells were incubated with FITC-labeled ACM-SSe and RCM-SSe for 2 h and analyzed by confocal laser scanning microscopy (CLSM) and flow cytometry (FCM).…”

Section: Targeting Studymentioning

confidence: 99%

Preparation of Biomimetic Selenium–Baicalein Nanoparticles and Their Targeted Therapeutic Application in Nonsmall Cell Lung Cancer

Shi,

Wang,

et al. 2024

Mol. Pharmaceutics

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In this study, we prepared bionic selenium–baicalein nanoparticles (ACM-SSe–BE) for the targeted treatment of nonsmall cell lung cancer. Due to the coating of the A549 membrane, the system has homologous targeting capabilities, allowing for the preparation of target tumor cells. The borate ester bond between selenium nanoparticles (SSe) and baicalein (BE) is pH-sensitive and can break under acidic conditions in the tumor microenvironment to achieve the targeted release of BE at the tumor site. Moreover, SSe further enhances the antitumor effect of BE by increasing the production of ROS in tumor cells. Transmission electron microscopy (TEM) images and dynamic light scattering (DLS) showed that the ACM-SSe–BE had a particle size of approximately 155 ± 2 nm. FTIR verified the successful coupling of SSe and BE. In vitro release experiments indicated that the cumulative release of ACM-SSe–BE at pH 5.5 after 24 h was 69.39 ± 1.07%, which was less than the 20% release at pH 7.4, confirming the pH-sensitive release of BE in ACM-SSe–BE. Cell uptake experiments and in vivo imaging showed that ACM-SSe–BE had good targeting ability. The results of MTT, flow cytometry, Western blot, and cell immunofluorescence staining demonstrated that ACM-SSe–BE promoted A549 cell apoptosis and inhibited cell proliferation. The in vivo antitumor results were consistent with those of the cell experiments. These results clearly suggested that ACM-SSe–BE will be a promising bionic nanosystem for the treatment of nonsmall cell lung cancer.

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Section: Targeting Studymentioning

confidence: 99%