Enzyme electrodes for determination of total phenolic capacity of red wines

Abstract

A tyrosinase-modified electrode, based on physical entrapment of enzyme in copolymer matrix of decanedioic acid bis(4-pyrrol-1-yl-phenyl) ester (DAPE) with pyrrole was constructed and used to investigate the total amount of phenolics in red wines. Kinetic parameters, V max , and K m , were determined. Also, temperature, pH optimizations, operational stability and shelf life of the enzyme electrode were examined. Surface morphologies of the films were studied by scanning electron microscope.

An alternative supporting electrolyte for enzyme immobilization in conducting polymers

“…Sodium dodecyl sulfate (SDS) is one of the common supporting electrolytes used in synthesis of conducting polymers. It is especially preferred during the construction of enzyme-modified conducting polymers to construct enzyme electrodes [8][9][10][11][12][13]. SDS is particularly interesting as an activating agent because few enzymes are known to have increasing activity by SDS.…”

Chitosan‐grafted poly(hydroxyethyl methacrylate‐co‐glycidyl methacrylate) membranes for reversible enzyme immobilization

“…[5][6][7][8] Among the various immobilization techniques available, adsorption may have a higher commercial potential than other methods because the adsorption process is simpler, less expensive, retains a high catalytic activity, and most importantly the support could be repeatedly reused after inactivation of the immobilized enzyme. [9][10][11] A number of methods for reversible immobilization of enzymes have been reported in the literatures. [11][12][13] However, the adsorption is generally not very strong and some of the adsorbed enzyme is desorbed during washing and operation.…”