Enzyme feeding strategies for better fed-batch enzymatic hydrolysis of empty fruit bunch

Sugiharto, Yohanes Eko Chandra; Harimawan, Ardiyan; Kresnowati, Made Tri Ari Penia; Purwadi, Ronny; Mariyana, Rina; Andry,; Fitriana, Hana Nur; Hosen, Hauna Fathmadinda

doi:10.1016/j.biortech.2016.01.113

Cited by 43 publications

(27 citation statements)

References 24 publications

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“…Corrêa, Badino, and Cruz (2016) reached an energy consumption reduction of 52% using fed-batch strategies on the enzymatic saccharification of steam pretreated sugarcane on a stirred tank bioreactor with two Elephant Ear impellers. On the other hand, Sugiharto et al (2016) stated that fed-batch mode can reduce cellulase inhibition by non-productive enzyme binding to lignin. The researchers found out that feed-batch strategy presented a positive effect on enzymatic hydrolysis of steam pretreated empty fruit bunch at 25% (w/v) solid loading.…”

Section: 322mentioning

confidence: 99%

Enhancement and modeling of enzymatic hydrolysis on cellulose from agave bagasse hydrothermally pretreated in a horizontal bioreactor

Pino

Rodríguez-Jasso

Michelin

et al. 2019

Carbohydrate Polymers

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A B S T R A C TOne of the major challenges in biofuels production from lignocellulosic biomass is the generation of high glucose titers from cellulose in the enzymatic hydrolysis stage of pretreated biomass to guarantee a cost-effective process. Therefore, the enzymatic saccharification on cellulose at high solid loading is an alternative. In this work, the agave bagasse was hydrothermally pretreated and optimized at 194°C/30 min, obtaining a pretreated solid rich in cellulose content (> 46.46%), and subjected to enzymatic hydrolysis at high solid levels. A horizontal bioreactor was designed for enzyme saccharification at high solid loadings [25% (w/v)]. The bioreactor improved mixing efficiency, with cellulose conversions up to 98% (195.6 g/L at 72 h). Moreover, mathematical modeling of cellulase deactivation demonstrated that cellulases lose most of their initial activity in the first hours of the reaction. Also, cellulose was characterized by X-ray diffraction, and the pretreated solids were visualized using scanning electron microscopy.

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Section: 322mentioning

confidence: 99%

Enhancement and modeling of enzymatic hydrolysis on cellulose from agave bagasse hydrothermally pretreated in a horizontal bioreactor

Pino

Rodríguez-Jasso

Michelin

et al. 2019

Carbohydrate Polymers

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“…Yet another set of studies claimed that enzyme splitting was more significant than whole enzyme feeding in late hours of the process. Sugiharto et al (2016) showed that the glucose concentration was higher for the first 40 h (25% total solids loadings was operated) when the whole enzyme was added at the beginning as compared with the concentration in the enzyme splitting. The glucose concentration was higher for the hydrolysis between 40 and 120 h with the enzyme splitting, as compared with the former one.…”

Section: Substrate Plus Enzyme Splitting: Optimization Of the Sizes Fmentioning

confidence: 99%

Improved Enzymatic Hydrolysis of Pilot Scale Pretreated Rice Straw at High Total Solids Loading

et al. 2018

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Enzymatic hydrolysis at high solids loading has the potential to reduce both capital and operational expenditures. Here, pretreatment of rice straw (PRS) with dilute acid was carried out at a pilot scale (250 kg per day) at 162 • C for 10 min and 0.35% acid concentration, followed by enzymatic hydrolysis at different total solids loadings. It showed that although the total sugar concentration increased from 48 to 132 g/l, glucan conversion reduced by 27% (84-66.2%) with increasing solids from 5 to 20% in batch mode. Therefore, two different fed-batch approaches were evaluated to improve the glucan conversion by the sequential addition of a substrate and/or enzyme. At 20% solid loadings and a 3 filter paper units/g enzyme dosage, the highest glucan conversion obtained was 66% after 30 h of hydrolysis in batch mode. However, in an optimized fed-batch approach, the glucan yield was improved to 70% by simply dividing the substrate feeding into three batches, that is, 50% at 0 h, 25% each after 4 h, and 8 h of hydrolysis reaction. The addition of surfactant (Ecosurf E6) further improved the conversion to 72% after 30 h. The role of critical factors, that is, inhibitors, enzyme-lignin binding, and viscosity, was investigated during the course of hydrolysis in the batch and fed-batch approaches. This study suggests a sustainable approach for improved hydrolysis at high solids loadings by fine-tuning a simple process.

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“…Fresh Brought to you by | Loughborough University Authenticated Download Date | 11/27/18 2:53 PM solids of 7% were consecutively added at the intervals of 6, 12, and 24 h to achieve maximum 33% solid loading, 129.50 g/l glucose concentration, and 56.03 g/l xylose concentration at the end of the process. Sugiharto et al (2016) clearly highlighted that enzyme feeding strategies can play an important role in fed-batch enzymatic hydrolysis process. In this study, commercial Cellic CTec 2 enzyme with dosages of 185 FPU/ml from Novozymes was implemented on 25% solid loading for 40 h to increase the digestibility and produce glucose concentration up to 26% and 12%.…”

Section: Hydrolysis Methodsmentioning

confidence: 99%

Lignocellulosic bioethanol production: prospects of emerging membrane technologies to improve the process – a critical review

Dey

Pal

Kevin

et al. 2018

Reviews in Chemical Engineering

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AbstractTo meet the worldwide rapid growth of industrialization and population, the demand for the production of bioethanol as an alternative green biofuel is gaining significant prominence. The bioethanol production process is still considered one of the largest energy-consuming processes and is challenging due to the limited effectiveness of conventional pretreatment processes, saccharification processes, and extreme use of electricity in common fermentation and purification processes. Thus, it became necessary to improve the bioethanol production process through reduced energy requirements. Membrane-based separation technologies have already gained attention due to their reduced energy requirements, investment in lower labor costs, lower space requirements, and wide flexibility in operations. For the selective conversion of biomasses to bioethanol, membrane bioreactors are specifically well suited. Advanced membrane-integrated processes can effectively contribute to different stages of bioethanol production processes, including enzymatic saccharification, concentrating feed solutions for fermentation, improving pretreatment processes, and finally purification processes. Advanced membrane-integrated simultaneous saccharification, filtration, and fermentation strategies consisting of ultrafiltration-based enzyme recycle system with nanofiltration-based high-density cell recycle fermentation system or the combination of high-density cell recycle fermentation system with membrane pervaporation or distillation can definitely contribute to the development of the most efficient and economically sustainable second-generation bioethanol production process.

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Enzyme feeding strategies for better fed-batch enzymatic hydrolysis of empty fruit bunch

Cited by 43 publications

References 24 publications

Enhancement and modeling of enzymatic hydrolysis on cellulose from agave bagasse hydrothermally pretreated in a horizontal bioreactor

Enhancement and modeling of enzymatic hydrolysis on cellulose from agave bagasse hydrothermally pretreated in a horizontal bioreactor

Improved Enzymatic Hydrolysis of Pilot Scale Pretreated Rice Straw at High Total Solids Loading

Lignocellulosic bioethanol production: prospects of emerging membrane technologies to improve the process – a critical review

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