Enzyme-free and label-free ultra-sensitive colorimetric detection of Pb2+ using molecular beacon and DNAzyme based amplification strategy

Yun, Wen; Cai, Dingzhou; Jiang, Jiali; Zhao, Pengxiang; Huang, Yu; Ge, Shenguang

doi:10.1016/j.bios.2016.01.053

Cited by 98 publications

(19 citation statements)

References 34 publications

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“…The gold nanoparticles aggregate without the stabilizing single-stranded DNA ligands, which shifts the surface plasmon resonance, resulting in a purple solution. This detection scheme has a reported LOD of 20 pM [75]. Similar nanoparticle-based colorimetric sensing strategies have been demonstrated in hydrogel systems.…”

Section: Colorimetric Sensorsmentioning

confidence: 89%

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DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells

McGhee

Loh

2017

Current Opinion in Biotechnology

128

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The on-site and real-time detection of metal ions is important for environmental monitoring and for understanding the impact of metal ions on human health. However, developing sensors selective for a wide range of metal ions that can work in the complex matrices of untreated samples and cells presents significant challenges. To meet these challenges, DNAzymes, an emerging class of metal ion-dependent enzymes selective for almost any metal ion, have been functionalized with fluorophores, nanoparticles and other imaging agents and incorporated into sensors for the detection of metal ions in environmental samples and for imaging the metal ions in living cells. Herein, we highlight the recent developments of DNAzyme-based fluorescent, colorimetric, SERS, electrochemical and electrochemiluminscent sensors for metal ions for these applications.

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Section: Colorimetric Sensorsmentioning

confidence: 89%

“…Most of these sensor designs take advantage of recent progress in the field of nanotechnology to couple activity of the DNAzyme with a colorimetric output. Yun et al [75] designed a sensor which uses a DNAzyme to cleave a DNA hairpin with low stability (Fig. 2A).…”

Section: Colorimetric Sensorsmentioning

confidence: 99%

DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells

McGhee

Loh

2017

Current Opinion in Biotechnology

128

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“…The first DNAzyme for RNA cleavage was reported in 1994 using an in vitro selection designed with metal ions (Breaker and Joyce, 1994). Since then, many DNAzymes have been selected and applied to detect metals such as Zn 2+ , Cu 2+ , UO 2+ , Pb 2+ , Ag + , and Hg 2+ (Liu and Lu, 2007;Luo et al, 2012;Endo et al, 2015;Saran and Liu, 2016;Yun et al, 2016). Besides, in recent years, many RNA-cleaving fluorogenic DNAzymes (RFDs) have been selected through crude extracellular mixtures (CEM) and detect common bacteria such as Escherichia coli, Klebsiella Pneumoniae, Helicobacter pylori, Bacterial pathogen, and Legionella pneumophila (Zhang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Detection of Vibrio vulnificus in Seafood With a DNAzyme-Based Biosensor

Fan

Tian

et al. 2021

Front. Microbiol.

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Vibrio vulnificus is an important pathogenic bacterium that is often associated with seafood-borne illnesses. Therefore, to detect this pathogen in aquatic products, a DNAzyme-based fluorescent sensor was developed for the in vitro detection of V. vulnificus. After screening and mutation, a DNAzyme that we denominated “RFD-VV-M2” exhibited the highest activity, specificity, and sensitivity. The limit of detection was 2.2 × 103 CFU/ml, and results could be obtained within 5–10 min. Our findings suggested that the target of DNAzyme RFD-VV-M2 was a protein with a molecular weight between 50 and 100 kDa. The proposed biosensor exhibited an excellent capacity to detect marine products contaminated with V. vulnificus. Therefore, our study established a rapid, simple, sensitive, and highly specific detection method for V. vulnificus in aquatic products.

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“…Therefore, microanalysis of cadmium ion (Cd 2+ ) and lead ion (Pb 2+ ) in water environment monitoring has become the focus of human attention. Many methods can be used for the determination of Cd 2+ and Pb 2+ ions in complicated water environments, such as colorimetric sensors [6][7][8], electrochemical analysis [9][10][11], fluorescent sensors [12][13][14], Raman scattering sensors [15], etc.…”

Section: Introductionmentioning

confidence: 99%

ZnSe quantum dot based ion imprinting technology for fluorescence detecting cadmium and lead ions on a three-dimensional rotary paper-based microfluidic chip

Zhou

et al. 2020

Sensors and Actuators B: Chemical

125

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In this study, a newly fluorescent ZnSe quantum dots (QDs) with ion imprinting technology was firstly realized on the three-dimensional (3D) rotary paper-based microfluidic chip platform which can be used to realize specific and multiplexed detection of Cadmium ions (Cd 2+ ) and Lead ions (Pb 2+ ). Compared to CdTe quantum dots, ZnSe quantum dots are less toxic and more environmental friendly. In addition, this design improved the portability of the device by transferred the liquid phase of ZnSe QDs@ion imprinted polymers to solid glass fiber paper. Moreover, the 3D rotary microfluidic chip (μPADs) showed great advantages including low cost, simple and fast facile operation, multiplexed detection, and showed good sensitivity and selectivity. Under optimal experiment conditions, our proposed method was enabled to realize specific and multi-channel determination of Cd 2+ and Pb 2+ ions. The developed sensor of Cd 2+ μPADs provided a linear response from 1 to 70 μg/L with a lower detection limit of 0.245 μg/L, and Pb 2+ μPADs provided a linear response from 1 to 60 μg/L with a lower detection limit of 0.335 μg/L, respectively. Excitingly, this newly designed 3D rotary μPADs exhibited quantitative information conveniently, which showed the promising application prospects to rapid testing target metal ions in environmental in the future.

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Enzyme-free and label-free ultra-sensitive colorimetric detection of Pb2+ using molecular beacon and DNAzyme based amplification strategy

Cited by 98 publications

References 34 publications

DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells

DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells

Detection of Vibrio vulnificus in Seafood With a DNAzyme-Based Biosensor

ZnSe quantum dot based ion imprinting technology for fluorescence detecting cadmium and lead ions on a three-dimensional rotary paper-based microfluidic chip

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