2018
DOI: 10.1021/acsami.8b02262
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Enzyme-free Target Recycling and Double-Output Amplification System for Electrochemiluminescent Assay of Mucin 1 with MoS2 Nanoflowers as Co-reaction Accelerator

Abstract: In this work, a sensitive electrochemiluminescent assay of mucin 1 (MUC1) was developed with the advantages of target recycling amplification strategy and effective MoS nanoflower (MoS NF)-based signal probe. Briefly, the target MUC1 triggered enzyme-free recycling and a double-output amplification process was executed to acquire masses of single-stranded DNA as a mimic target, which further participated in the catalytic hairpin assembly process for signal amplification. Meanwhile, MoS NFs were prepared as an … Show more

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Cited by 63 publications
(23 citation statements)
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“…It has three Raman vibration bands, a disordered (D) peak at ~1345 cm −1 , a graphitic carbon (G) peak at ~1594 cm −1 , and a 2D peak at ~2677 cm −1 under 532 nm laser excitation (Figure 1C), while only a D peak and a G peak were observed on its Raman spectrum under 633 nm laser excitation (Figure 1D). X‐ray photoelectron spectrometer (XPS) characterization results in Figure 1E showed the characteristic peaks of Au4f (83.48 eV for Au4f 7/2 and 87.18 eV for Au4f 5/2 , respectively), [ 50‐51 ] Ag3d (367.58 eV for Ag3d 5/2 and 373.58 eV for Ag3d 3/2 , respectively) [ 52‐53 ] and C1s (283.88 eV). Raman signals of GIAAN's G peak in H 2 O, methanol (Me), cyclohexane ethyl (CH), acetate (EA), petroleum ether (PE) and dichloromethane (DCM) showed little difference before and after 12 h, indicating the superb stability of GIAAN (Figure 1F).…”
Section: Resultsmentioning
confidence: 99%
“…It has three Raman vibration bands, a disordered (D) peak at ~1345 cm −1 , a graphitic carbon (G) peak at ~1594 cm −1 , and a 2D peak at ~2677 cm −1 under 532 nm laser excitation (Figure 1C), while only a D peak and a G peak were observed on its Raman spectrum under 633 nm laser excitation (Figure 1D). X‐ray photoelectron spectrometer (XPS) characterization results in Figure 1E showed the characteristic peaks of Au4f (83.48 eV for Au4f 7/2 and 87.18 eV for Au4f 5/2 , respectively), [ 50‐51 ] Ag3d (367.58 eV for Ag3d 5/2 and 373.58 eV for Ag3d 3/2 , respectively) [ 52‐53 ] and C1s (283.88 eV). Raman signals of GIAAN's G peak in H 2 O, methanol (Me), cyclohexane ethyl (CH), acetate (EA), petroleum ether (PE) and dichloromethane (DCM) showed little difference before and after 12 h, indicating the superb stability of GIAAN (Figure 1F).…”
Section: Resultsmentioning
confidence: 99%
“…All experimental data suggested the introduction of MoS2-based nanoprobes can efficiently improve the analytical performances, such as linear range, detection limit, analytical time, etc. Reprinted with permission from [52]. Copyright 2018, American Chemical Society.…”
Section: Ecl Sensorsmentioning
confidence: 99%
“…138 To minimize these drawbacks as much as possible, enzyme-free recycling amplification strategies have been developed. 136 Moreover, these strategies can also be integrated into a biosensing approach such as electrochemical 137 or SERS 139 instead of using bulky instruments (e.g., spectrophotometer 140 ) to diagnose diseases such as cancer 137 or kidney diseases. 141 For instance, this DNase-I-assisted recycling signal amplification strategy was applied for the determination of AKI at an early stage by targeting NGAL biomarker.…”
Section: •−mentioning
confidence: 99%
“…However, enzyme involvement protocols also increase the system's complexity and cost due to the requirement of optimal conditions for efficient reaction and a well-equiped facility for the production, yet an enzyme-free strategy would be an alternative to eliminate these drawbacks. 136 Overall, these aforementioned issues along with potential strategies would enable improvement of both current and future diagnostic procedures for AKI diagnosis. Due to their universal nature, these strategies cannot be limited to AKI, and they can be adapted for the other diseases and biosensing strategies as well.…”
Section: •−mentioning
confidence: 99%