Enzyme Fueled Dissipative Self‐assembly of Guanine Functionalized Molecules and Their Cellular Behaviour

Abstract

Generally, an esterase lipase enzyme can hydrolyze specific substrates called esters in an aqueous solution. Herein, we investigate how a G‐quadruplex self‐assembly affects the hydrolysis equilibrium in reverse. The biocatalyst, lipase, activates the individual building‐blocks through fuel consumption, causing them to undergo a higher degree of self‐organization into nanofibers within spheres. We have synthesized five peptide‐lipid‐conjugated guanine base functionalized molecules to explore how the equilibrium can be shifted through reverse hydrolysis. Among these, NAC5 self‐assembled into a G‐quadruplex structure which has been confirmed by various spectroscopic techniques. The wide‐angle powder XRD, ThT dye binding assay and circular dichroism study is carried out to support the presence of the G‐quadruplex structure. The biocatalytic formation of nanofibers enclosed spheres is analyzed using CLSM, FE‐SEM and HR‐TEM experiments. Additionally, we assess the biocompatibility of the enzyme fueled dissipative self‐assembled fibers enclosed spheres, as they have potential applications as a biomaterial in protocells. MTT assay is performed to check the cytotoxicity of G‐quadruplex hydrogel, using HEK 293 and McCoy cell lines for viability assessment. Finally, the utility of the novel NAC5 hydrogel as a wound repairing biomaterial is demonstrated by cell migration experiment in a scratch assay.