Enzyme immobilization by condensation copolymerization into crosslinked polyacrylamide gels

Pollak, Alfred; Blumenfeld, Hugh; Wax, Michael; Baughn, Richard L.; Whitesides, George M.

doi:10.1021/ja00540a026

Cited by 263 publications

(142 citation statements)

References 3 publications

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“…1A) after exhaustive aqueous extraction. IR spectroscopy showed progressive loss of oxysuccinimide groups during reaction of TERP and TERP5 with collagen, consistent with the expected crosslinking reaction (11). The composite collagen-copolymer matrices, molded to the curvature and dimensions of a cornea (Fig.…”

Section: Resultssupporting

confidence: 73%

“…After purification and solution in PBS, the cloud point was Ͼ55°C. Acryloxysuccinimide groups spontaneously crosslink proteins and anchor peptides through their primary amine groups (11). A second copolymer (designated TERP5) was synthesized from TERP by reacting some of its acryloxysuccinimide groups with the peptide YIGSR (YIGSR-NH 2 , Nova Biochem, Laufelfingen, Switzerland) in N,N-dimethyl formamide (48 h at 21°C).…”

Section: Methodsmentioning

confidence: 99%

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Cellular and nerve regeneration within a biosynthetic extracellular matrix for corneal transplantation

Li¹,

Carlsson²,

Lohmann³

et al. 2004

American Journal of Ophthalmology

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Our objective was to determine whether key properties of extracellular matrix (ECM) macromolecules can be replicated within tissue-engineered biosynthetic matrices to influence cellular properties and behavior. To achieve this, hydrated collagen and Nisopropylacrylamide copolymer-based ECMs were fabricated and tested on a corneal model. The structural and immunological simplicity of the cornea and importance of its extensive innervation for optimal functioning makes it an ideal test model. In addition, corneal failure is a clinically significant problem. Matrices were therefore designed to have the optical clarity and the proper dimensions, curvature, and biomechanical properties for use as corneal tissue replacements in transplantation. In vitro studies demonstrated that grafting of the laminin adhesion pentapeptide motif, YIGSR, to the hydrogels promoted epithelial stratification and neurite in-growth. Implants into pigs' corneas demonstrated successful in vivo regeneration of host corneal epithelium, stroma, and nerves. In particular, functional nerves were observed to rapidly regenerate in implants. By comparison, nerve regeneration in allograft controls was too slow to be observed during the experimental period, consistent with the behavior of human cornea transplants. Other corneal substitutes have been produced and tested, but here we report an implantable matrix that performs as a physiologically functional tissue substitute and not simply as a prosthetic device. These biosynthetic ECM replacements should have applicability to many areas of tissue engineering and regenerative medicine, especially where nerve function is required.regenerative medicine ͉ tissue engineering ͉ cornea ͉ implantation ͉ innervation

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Section: Resultssupporting

confidence: 73%

Section: Methodsmentioning

confidence: 99%

Cellular and nerve regeneration within a biosynthetic extracellular matrix for corneal transplantation

Li¹,

Carlsson²,

Lohmann³

et al. 2004

American Journal of Ophthalmology

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show abstract

“…To this end a popular approach is to first modify the surface with amino silane reagents which results in a uniform layer of covalently attached primary amines. These can then be easily functionalized to the appropriate activated group for reaction with amino-, phosphate-or acrylamide-modified DNA [7][8][9]. Highly selective copper-catalyzed alkyne-azide cycloaddition (CuCAAC) has also been demonstrated for microarray DNA immobilization [10] while non-covalent attachment can be employed using biotin-avidin interactions [11] or magnetically functionalized primers [12,13].…”

Section: Approaches For Solid-phase Dna Amplification Microarray Dna mentioning

confidence: 99%

Recent developments in nucleic acid identification using solid-phase enzymatic assays

Khodakov

Ellis

2014

Microchim Acta

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“…Afterwards, the comonomer was purified as described by Pollak et al [16] The prepolymer, PVCL-co-NASI was obtained as follows: VCL was dissolved in dry 1,4-dioxane under nitrogen. The solution was heated to 70 8C, and AIBN was added (1 mol-%).…”

Section: Pvcl-co-nasi Precursormentioning

confidence: 99%

Thermo‐Responsive and Emulsifying Properties of Poly(N‐vinylcaprolactam) Based Graft Copolymers

Verbrugghe

Bernaerts

Prez

2003

Macro Chemistry & Physics

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Thermo‐responsive graft copolymers have been synthesized based on a poly(N‐vinylcaprolactam) (PVCL) backbone and either hydrophilic poly(ethylene oxide) (PEO) or hydrophobic poly(tetrahydrofuran) (PTHF) side chains. The phase separation behavior of the graft polymers in water was studied by transmittance measurements and compared to that of the corresponding swollen segmented polymer networks and aqueous solutions of both polymers. The influence of the concentration and length of the grafts on the cloud point temperature (TCP) has been demonstrated. PVCL‐g‐PTHF copolymers have been synthesized by using the macromonomer technique, i.e. the radical copolymerization of VCL with a PTHF macromonomer. A special feature of these amphiphilic graft copolymers is their ability to stabilize aqueous emulsions below the TCP and to suddenly break them above the TCP. PVCL‐g‐PEO copolymers were prepared by a grafting onto method. First, succinimide groups were introduced in the backbone, to which amino terminated PEO chains were grafted in the second step. This leads to di‐hydrophilic copolymers that become amphiphilic after heating their aqueous solutions above the TCP.

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Enzyme immobilization by condensation copolymerization into crosslinked polyacrylamide gels

Cited by 263 publications

References 3 publications

Cellular and nerve regeneration within a biosynthetic extracellular matrix for corneal transplantation

Cellular and nerve regeneration within a biosynthetic extracellular matrix for corneal transplantation

Recent developments in nucleic acid identification using solid-phase enzymatic assays

Thermo‐Responsive and Emulsifying Properties of Poly(N‐vinylcaprolactam) Based Graft Copolymers

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