Enzyme immobilization on cellulose matrixes

Liu, Yue; Chen, Jonathan Y.

doi:10.1177/0883911516637377

Cited by 99 publications

(57 citation statements)

References 92 publications

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“…Carbon nanotubes have been receiving a lot of attention lately due to their excellent mechanical, chemical and thermal resistance, an ordered nanoporous structure, large surface area, biocompatibility, and the possibility of surface modifications to improve interactions with enzymes (Zdarta et al 2018). These materials are unique since they enhance transfer of electrons between the carrier and enzyme as demonstrated by study of Liu, Wang, Zhao, Xu, and Dong (2005). They immobilized glucose oxidase on the carbon nanotubes/chitosan carriers and used this system as a glucose biosensor (Liu et al 2005).…”

Section: Carbon Nanotubesmentioning

confidence: 99%

“…These materials are unique since they enhance transfer of electrons between the carrier and enzyme as demonstrated by study of Liu, Wang, Zhao, Xu, and Dong (2005). They immobilized glucose oxidase on the carbon nanotubes/chitosan carriers and used this system as a glucose biosensor (Liu et al 2005). Mohiuddin, Arbain, Islam, Ahmad, and Ahmad (2016) applied covalently immobilized α-glucosidase on carbon nanotubes as a biosensor for measuring the antidiabetic potential of medicinal plants (Mohiuddin et al 2016).…”

Section: Carbon Nanotubesmentioning

confidence: 99%

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The Enzyme Immobilization: Carriers and Immobilization methods

Spasojević¹,

Prodanović²,

Pantić³

et al. 2020

JEPM

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Strategies based on the enzyme application are increasingly replacing the conventionalchemical procedures because of their efficiency, quicker performance and environmentalprotection. However, natural enzymes can rarely be used in industrysince their beneficial features cannot endure the industrial conditions. Additionaldrawbacks of natural enzymes are their inhibition by reaction products and difficultyto be removed from the reaction mixture. The most promising techniqueto substantially improve the enzyme properties, such as activity, pH, thermal andorganic-solvent stability, reusability and storage stability, in non-natural environmentsis by the enzyme immobilization. In this review different techniques usedto immobilize enzymes to inert carriers were summarized. Different materials ofboth the organic and inorganic origin were used as carriers for the enzyme immobilization.A class of new materials where the enzyme performance was enhancedby combining different classical materials and shaping in specific forms was alsosummarized.

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Section: Carbon Nanotubesmentioning

confidence: 99%

Section: Carbon Nanotubesmentioning

confidence: 99%

The Enzyme Immobilization: Carriers and Immobilization methods

Spasojević¹,

Prodanović²,

Pantić³

et al. 2020

JEPM

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“…The characteristics of cellulose such as biocompatibility, biodegradability, chemical stabilization and low contamination risk to the environment have made it a matter of interest for enzyme immobilization (Klemm, et al, 2005). There are different methods for treatment of cellulose to make it compatible for the enzyme immobilization like esterification, periodate oxidation, 2, 2, 6, 6-tetramethylpyperidin-1-oxyl (TEMPO) mediated oxidation etc (Liu & Chen, 2016).…”

Section: Introductionmentioning

confidence: 99%

Establishment of a functional and sustainable bio-system engineering process for the saccharification of starch with industrial focus

Saha

Islam

Zohora

et al. 2019

Jahangirnagar Univ. J. Biol. Sci.

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Enzyme immobilization provides an excellent opportunity to use the enzymes for several times with greater productivity. The main aim of the present study is the establishment of a functional and sustainable bio-system engineering process based on immobilized α-amylase enzyme for the saccharification of starch with industrial focus as well as cost effective sustainable bioprocess system by using indigenous industrial waste materials as carrier agents. In this study, α-amylase was immobilized in different types of support matrices like alginate, sawdust, sugarcane bagasse and the activity of immobilized enzymes were analyzed for the breakdown of starch. The experimental results showed that the productivity of immobilized enzyme was around 2.3 times higher than the free enzyme. Conjugates of bagasse-alginate showed the highest result. A fixed batch immobilized enzyme bioreactor had been developed which could be used for the production of various valuable products in the industrial scale. Jahangirnagar University J. Biol. Sci. 8(1): 45-55, 2019 (June)

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“…Cellulose is inert and resistant to the adhesion of most biomolecules,a nd must therefore be chemically activated prior to bioconjugation of enzymes. [3] Many of these methods however,a re prone to enzyme inactivation and low activity yields,and no single conjugation chemistry works for all enzymes. [2] Theh ydroxy groups on cellulose must be functionalized before covalent attachment.…”

mentioning

confidence: 99%

“…[2] Theh ydroxy groups on cellulose must be functionalized before covalent attachment. [3] Many of these methods however,a re prone to enzyme inactivation and low activity yields,and no single conjugation chemistry works for all enzymes.…”

mentioning

confidence: 99%

A Modular Approach for Interlocking Enzymes in Whatman Paper

et al. 2018

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A potentially universal approach is presented for enzyme attachment to cellulose that significantly enhances enzyme stability while retaining high activity, and involves no chemical functionalization of cellulose. Bovine serum albumin (BSA) was interlocked in cellulose to form a protein-friendly surface (named BSA-Paper), while also providing COOH and NH groups for subsequent attachment of enzymes. The desired enzyme is then mixed with additional BSA and interlocked on BSA-Paper. The second BSA layer dilutes and crosslinks the enzyme for improved stability. Laccase was tested as a model enzyme for interlocking on BSA-Paper, and was found to retain over 100 % activity and was 240 times more stable at 25 °C (half life=180 d) than laccase. This new approach was also tested with a few other enzymes with encouraging results, thus providing a potentially universal method for stabilization of enzymes on cellulose with retention of high activities.

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Enzyme immobilization on cellulose matrixes

Cited by 99 publications

References 92 publications

The Enzyme Immobilization: Carriers and Immobilization methods

The Enzyme Immobilization: Carriers and Immobilization methods

Establishment of a functional and sustainable bio-system engineering process for the saccharification of starch with industrial focus

A Modular Approach for Interlocking Enzymes in Whatman Paper

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