Enzyme immobilization on nitrocellulose film for pH-EGFET type biosensors

Yin, Li-Te; Lin, Yen-Liang; Leu, Ying-Chen; Hu, Chih-Yu

doi:10.1016/j.snb.2010.04.042

Cited by 29 publications

(12 citation statements)

References 25 publications

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“…Urease is immobilized on the sensing membrane by different methods such as physical adsorption, entrapment, covalent bonding and cross-linking [108][109][110][111][112]. Lately, in order to simplify the production process and to improve the efficiency of the enzyme/analyte bonds, urea sensors based on EGFET have been developed [82,113]. Covalent bonding is a very effective method of immobilization due to the strong interaction that comes between the enzymes and the biological material, Being of clinical interest (especially in the blood), EGFET-based urea sensors can represent a promising technology (especially for the development of disposable devices) that indirectly detects urea concentration through the evaluation of pH variation as a result of an enzymatically catalyzed reaction.…”

Section: Urea Sensorsmentioning

confidence: 99%

EGFET Based Sensors for Bioanalytical Applications: A Review

Pullano¹,

Critello²,

Mahbub³

et al. 2018

Preprint

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Since 1970s, a great deal of attention has been paid to the development of semiconductor–based biosensors because of the numerous advantages they offer, including high sensitivity, faster response time, miniaturization, and low–cost manufacturing for quick biospecific analysis with reusable features. Commercial biosensors have become highly desirable in the fields of medicine, food, environmental monitoring as well as military applications (e.g., Hoffmann–La Roche, Abbott Point of Care, Orion High technologies, etc.), whereas increasing concerns on the food safety and health issues have resulted in the introduction of novel legislative standards for these sensors. Numerous devices have been developed for monitoring of biological–processes such as nucleic–acid hybridization, protein–protein interaction, antigen–antibody bonds and substrate–enzyme reactions, just to name a few. Since 1980s scientific interest moved to the development of semiconductor–based devices which also include integrated front–end electronics, such as the extended–gate–field–effect–transistor biosensor which is one of the first miniaturized chemical sensors. This work is intended to be a review of the state of the art focused on the development of biosensors based extended–gate–field–effect–transistor within the field of bioanalytical applications, which will highlight the most recent research works reported in the literature. Moreover, a comparison among the diverse EGFET devices will be presented giving particular attention to the materials and technologies.

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Section: Urea Sensorsmentioning

confidence: 99%

EGFET Based Sensors for Bioanalytical Applications: A Review

Pullano¹,

Critello²,

Mahbub³

et al. 2018

Preprint

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“…In civilian field, NC was used as an additive in paints and lacquer coatings for automobile and wood furniture [2,3]. NC has special application in biosensor to be used as a carrier of protein microarray for its neutral effect on proteins and immobilization for enzyme [4][5][6]. However, the volatile nitric acid ester groups (À OÀ NO 2 ) and hydroxyl groups of NC are thermal instability and sensitive to ambient humidity, respectively, which leads to many serious accidents involving transportation and storage [7].…”

Section: Introductionmentioning

confidence: 99%

Preparation of an Electrochemical Sensor Based on Surface Molecularly Imprinted Polymers Modified Electrode and its Application in Detection of Nitrocellulose

Meng

Yang

Xiao

2019

Propellants Explo Pyrotec

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In order to develop a novel method for the detection of nitrocellulose (NC), an electrochemical sensor based on silica surface molecularly imprinted polymers (SiO2/MIPs) modified electrode was prepared to detect NC. In addition, SiO2 non‐imprinted polymers‐based (SiO2/NIPs) sensor was prepared as a control. The response characteristic of SiO2/MIPs‐based sensor to NC was studied with [Fe(CN)6]3−/4− as a probe molecule. The SiO2/MIPs‐based sensor have an optimized current responsiveness to NC when it was prepared at temperature of 50 °C and 1 : 3 proportion of methacrylic acid to ethyleneglycol dimethacrylate. The current response of SiO2/MIPs‐based sensor is the most significant under the NC solution of 4.0 mg mL−1, incubation period of 1 min and [Fe(CN)6]3−/4− electrolyte of 5 mM. The SiO2/MIPs‐based sensor had the greatest response capacity for NC among the several analogues, which indicates that the SiO2/MIPs‐based sensor has a good specificity to NC. Furthermore, the SiO2/MIPs‐based sensor exhibited an excellent reproducibility in repeated measurements. Results show the prepared SiO2/MIPs‐based sensor can be a potential candidate for the detection of NC in the security checking and hazard control.

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“…2,3 Recently, it has been applied in the fabrication of specic biosensors, especially in protein microarrays of biochips for its neutral effect on proteins and immobilization of enzymes. [4][5][6][7] Nevertheless, most of the terrorist attacks occurring worldwide involve the misuse of NC and other explosives. Further, the preparation of paints and lacquer coatings may cause environmental contamination such as water pollution.…”

Section: Introductionmentioning

confidence: 99%