Enzyme-immobilized metal–organic framework nanosheets as tandem catalysts for the generation of nitric oxide

Ling, Pinghua; Qian, Caihua; Gao, Feng; Lei, Jianping

doi:10.1039/c8cc05068f

Cited by 57 publications

(25 citation statements)

References 40 publications

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“…Targeting the overexpression of glucose in cancer cells, a novel concept named starvation therapy is performed by glucose depletion with GOx . The desirable loading efficiency and well‐preserved enzymatic activity of GOx into MOFs, allows it to more efficiently biocatalyze the conversion of endogenous glucose in cancer cells . Meanwhile, it has been demonstrated that the combination of different therapeutic ways (e.g.…”

Section: Applicationsmentioning

confidence: 99%

“Armor‐Plating” Enzymes with Metal–Organic Frameworks (MOFs)

et al. 2020

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Cell‐free enzymatic catalysis (CFEC) is an emerging biotechnology that enable the biological transformations in complex natural networks to be imitated. This biomimetic approach allows industrial products such as biofuels and biochemical to be manufactured in a green manner. Nevertheless, the main challenge in CFEC is the poor stability, which restricts the effectiveness and lifetime of enzymes in sophisticated applications. Immobilization of the enzymes within solid carriers is considered an efficient strategy for addressing these obstacles. Specifically, putting an “armor‐like” porous metal–organic framework (MOF) exoskeleton tightly around the enzymes not only shields the enzymes against external stimulus, but also allows the selective transport of guests through the accessible porous network. Herein we present the concept of this biotechnology of MOF‐entrapped enzymes and its cutting‐edge applications.

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Section: Applicationsmentioning

confidence: 99%

“Armor‐Plating” Enzymes with Metal–Organic Frameworks (MOFs)

et al. 2020

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“…Metal-organic frameworks (MOFs) are a new kind of organic-inorganic hybrid porous material [23,24]. Since MOF materials are considered to be potent supporting matrices for enzyme immobilization, many kinds of commodity enzymes were immobilized successfully [25][26][27][28]. However, other researchers have spent their energy on the design of Zn 2+ , Cu 2+ or Fe 3+ -based MOFs for enzyme immobilization [29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Fast Immobilization of Human Carbonic Anhydrase II on Ni-Based Metal-Organic Framework Nanorods with High Catalytic Performance

Jiao

Sun

et al. 2020

Catalysts

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Carbonic anhydrase (CA) has received considerable attention for its ability to capture carbon dioxide efficiently. This study reports a simple strategy for immobilizing recombinant carbonic anhydrase II from human (hCA II) on Ni-based MOFs (Ni-BTC) nanorods, which was readily achieved in a one-pot immobilization of His-tagged hCA II (His-hCA II). Consequently, His-hCA II from cell lysate could obtain an activity recovery of 99% under optimal conditions. After storing for 10 days, the immobilized His-hCA II maintained 40% activity while the free enzyme lost 91% activity. Furthermore, during the hydrolysis of p-nitrophenyl acetic acid, immobilized His-hCA II exhibited excellent reusability and still retained more than 65% of the original activity after eight cycles. In addition, we also found that Ni-BTC had no fixation effect on proteins without histidine-tag. These results show that the Ni-BTC MOFs have a great potential with high efficiency for and specific binding of immobilized enzymes.

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“…There has been growing interest in the use of MOFs for tandem catalysis 36 38 with recent reports of MONs being used alongside nanoparticles and enzymes to facilitate multistep reactions. 9,10,39 As Cu(ABDC-PS) is partially functionalised with sulfonic acid groups, with basic amine sites remaining, we decided to investigate their ability to catalyse a well-known acid-base tandem reaction. 34,40,41 Cu(ABDC-PS) was tested as a catalyst for the acid hydrolysis of benzaldehyde dimethyl acetal (1) to benzaldehyde (2), followed by a Knoevenagel condensation to form benzylidene malononitrile (3).…”

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confidence: 99%