Enzyme immunoassay analysis of antibody specificities present in the circulating immune complexes of selected pathological sera

Characterization of autoantibody activities in sera anti-DNA antibody and circulating immune complexes from 12 systemic lupus erythematosus patients

“…Induced antibodies might participate in the immune complex formation, since we have observed that anti-ACT activity was the most expressed in CIC. Thus CIC could be formed by all these autoantibody populations cited above either complexed with rheumatoid factor (28) or by an antigenantibody reaction (7,29).Although not confirmed in humans, autoantibodies from sera, CIC and kidney eluates of (NZB x NZW) F1 lupus-prone mice were shown to react with DNA and immunoglobulin Fc and Fab fragments (21) and with some monoclonal antibodies (26,29).…”

“…Briefly, after washing with 2.5% PEG, precipitates were dissolved in 0.15 M NaCl, treated at acid pH to dissociate immune complexes, neutralized, and adjusted to a final volume of 4 ml with phosphate-buffered saline (PBS) before analysis of their antibody activities (7). The protein concentration was determined by the Biuret method.…”

“…In addition to these autoantibodies, those belonging to the family of natural polyreactive antibodies were also increased in sera and kidney eluates (6) as well as in circulating immune complexes (CIC) (7).…”

Prognostic Significance of Autoantibodies to Laminin in the Sera of Breast Cancer Patients: A Preliminary Report

“…Concentrations of autoantibodies to laminin were measured by enzyme immunoassay s previously described (11). Laminin antigen was highly purified from mouse EngelbrethHolm-Swarm sarcoma extracts by anion exchange chromatography and gel filtration s previously described (12).…”

Autoimmune complexes in human serum in presence of therapeutic amounts of intravenous immunoglobulins

“…Soluble plasma ICs have been observed in a number of autoimmune diseases, 15 but the possible formation of soluble IC containing IVIG and normal plasma proteins has not been specifically studied so far. It is possible that these ICs are rapidly cleared from the circulation after interaction with FcR-bearing cells, thus making them difficult to detect in the plasma of IVIG-treated patients.…”