Enzyme immunoassay method for total urinary follicle-stimulating hormone (FSH) beta subunit and its application for measurement of total urinary FSH

Qiu, Qing; Kuo, Andy; Todd, Heather; Dias, James A.; Gould, John E.; Overstreet, James W.; Lasley, Bill L.

doi:10.1016/s0015-0282(97)00475-5

Cited by 31 publications

(20 citation statements)

References 16 publications

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“…(2)). A persistent issue in the use of urinary and serum LH and FSH assays is concern over the effects of collection and storage conditions on dissociation of the alpha and beta (β) subunits of these hormones (3)(4)(5)(6). This is particularly relevant for large-scale population-level research where there may be considerable delay between specimen collection from individuals in their homes, or in the field, and time of assay.…”

Section: Introductionmentioning

confidence: 99%

“…However, preservatives have some limitations for population level research, including time and expense invested in the advance preparation of specimen collection tubes for home or field collections, potential assay interference, effects on other hormones to be measured in the same specimen, and the fact that dilution volume correction may be necessary for the final urinary result (3,13). Additionally, preservatives do not correct for any inter-subject variability that might exist in modification of LH and FSH between circulation in the blood and excretion in the urine (3).…”

Section: Introductionmentioning

confidence: 99%

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Urinary beta-luteinizing hormone and beta-follicle stimulating hormone immunoenzymometric assays for population research

Brindle

Miller

Shofer

et al. 2006

Clinical Biochemistry

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Objective-We developed assays for measurement of urinary βLH and βFSH under collection and storage conditions typical of non-clinical research settings.Design and Methods-IEMAs for free βLH and total βFSH were validated by standard methods. Stability of urinary βLH and βFSH was tested across freeze thaws and stored long-term at 4°C or −20°C, or short term at room temperature, and with heating to dissociate the subunits. Results-TheIEMAs exhibited acceptable parallelism, specificity, recovery (averaging 100% for βLH, 97% for βFSH), imprecision (maximum within-run and between run CVs, respectively, 4.8% and 25.7% for βLH, 5.6% and 17.0% for βFSH), and minimum detectable dose (2.5 pmol/L for βLH, 6.8 pmol/L for βFSH). Urine and serum measures were highly correlated (r = 0.95 for LH, 0.86 for FSH). There was no consistent decline with any storage type. Dissociation of subunits by heating was needed for βLH, but not βFSH. Conclusion-TheseIEMAs measure free βLH and total βFSH, overcoming inter-individual variability in, and collection and storage effects on, subunit dissociation, without the need for urine preservatives.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Urinary beta-luteinizing hormone and beta-follicle stimulating hormone immunoenzymometric assays for population research

Brindle

Miller

Shofer

et al. 2006

Clinical Biochemistry

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“…These biomarkers are metabolites of reproductive hormones. Under field conditions, multiple biomarkers may be needed for detection of complex reproductive outcomes such as pregnancy loss (6,8).…”

Section: Human Studiesmentioning

confidence: 99%

“…The assay that we previously developed for urinary Po metabolites (9) can be used to recognize the luteal phase of the cycle, and identification of the midcycle surge of FSH can be used to determine the approximate day of ovulation (8), and thus an implantation window in which hCG excretion is likely to be a result of pregnancy (6). An algorithm has been developed for detection of pregnancy using only the urinary FSH biomarker.…”

Section: Human Studiesmentioning

confidence: 99%

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Biomarkers for assessing human female reproductive health, an interdisciplinary approach.

Lasley

Overstreet

1998

Environ Health Perspect

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Identification of environmental hazards to reproductive health and characterization of the adverse outcomes necessitate a multidisciplinary approach. Epidemiologic studies are required for the identification of adverse health effects in human populations and then to confirm that specific exposures are responsible. Clinical studies are required to develop assays for reproductive biomarkers and to validate these assays prior to their application in the field. Assays for field use must be formatted and streamlined for large-scale applications and, whenever possible, computer algorithms should be developed to interpret biomarker data. Appropriate animal models must be identified, biomarker assays validated for that model, and animal experiments conducted to identify the mode of action and target organ of a putative reproductive toxicant. Finally, in vitro studies at the level of the cell and cell organelle are essential for mechanisms of toxicity to be clearly identified and understood. In this article we describe the interdisciplinary approach that we have developed for study of the effects of environmental agents on female reproductive functions. This effort requires specific skills of toxicologists, epidemiologists, physicians, biochemists, and physiologists.Environ Health Perspect 106 (Suppl 41:955-960 (1998). http.//ehpnetl.niehs.nih.gov/docs/1998/Suppl-4/955-9601asley/abstract.html

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Paraoxonase polymorphism and its effect on male reproductive outcomes among Chinese pesticide factory workers

et al. 1999

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Background Serum paraoxonase has been associated with the metabolism of organophosphate pesticides in humans. Molecular analysis of the human paraoxonase gene (PON1) has revealed that Arg192 homozygotes have a greater detoxifying capability than Gln192 homozygotes. We examined the effects of PON1 genotypes on male reproductive outcomes and its interaction with exposure to organophosphate pesticides. Methods We studied 60 Chinese pesticide‐factory workers and 89 textile‐factory workers who were unexposed to pesticides. The respective allele frequencies of Arg192 and Gln192 were 0.62 and 0.38. Pesticide exposure among 36 exposed subjects and 12 unexposed subjects, regardless of gender, was assessed by personal measurement of pesticide residues over an entire 8‐hr shift and measurement of urinary p‐nitrophenol level over a 24‐hr period. We analyzed semen and hormone data collected from male subjects. Results When the three PON1 genotypes were analyzed separately, a gene dose effect was not detected. We used the unexposed Arg192 homo/heterozygotes as the reference group, and re‐analyzed the data. Exposed Arg192 homo/heterozygotes had significantly lower sperm count (χ2=9.01, P < 0.01) and lower percentage of sperm with normal morphology (χ2=4.18, P < 0.05) than the reference group. Both unexposed Gln192 homozygotes (χ2=4.90, P < 0.05) and exposed Arg192 homo/heterozygotes (χ2=10.00, P < 0.01) showed significantly lower sperm concentrations than the reference group. In addition, exposed Arg192 homo/heterozygotes had significantly higher serum LH levels (χ2=7.94, P < 0.01) than the reference group. Conclusions Because of a small sample size, our findings are highly preliminary. Nevertheless, it calls for further investigation of the interaction between the PON1 genotype and organophosphate pesticide exposure on male reproductive outcomes. Am. J. Ind. Med. 36:379–387, 1999. © 1999 Wiley‐Liss, Inc.

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Enzyme immunoassay method for total urinary follicle-stimulating hormone (FSH) beta subunit and its application for measurement of total urinary FSH

Cited by 31 publications

References 16 publications

Urinary beta-luteinizing hormone and beta-follicle stimulating hormone immunoenzymometric assays for population research

Urinary beta-luteinizing hormone and beta-follicle stimulating hormone immunoenzymometric assays for population research

Biomarkers for assessing human female reproductive health, an interdisciplinary approach.

Paraoxonase polymorphism and its effect on male reproductive outcomes among Chinese pesticide factory workers

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