Enzyme Immunoassay of Hormones

Development of a microtitre plate enzyme immunoassay for the determination of progesterone

“…The microelisa test used in this study is based on the principle of the competitive technique for detection of small antigens outlined by Voller et al (1976). The competitive type of assay is the only one that has been used for steroid determination, since steroid molecules are too small to bind two antibody molecules as required in the sandwich assay technique (Van Weemen, Bosch, Dawson et al 1978). The only other microtitre plate EIA described for progesterone is an homologous assay with a sensitivity of 50pg/well and a limited change (0-14-0-03) in adsorbance over the figure 5.…”

Enzyme Immunoassay for Methionine‐enkephalin Sulfoxide

“…These have, in the main, utilized radiolabelled reagents. It is well established, however, that labelled enzymes can be usefully employed for the immunoassay of a wide variety of peptide molecules (Van Weemen et al 1978). The sensitivities achieved in these enzyme immunoassays approach or even exceed that of radio-immunoassays.…”

The use of an enzyme immunoassay for the detection of salmonellae in foods

“…A recent comprehensive review of coupling techniques has been compiled by Avrameas et al (1978). Applications of enzyme-labeled antibodies are broad and include the following: localization of specific intracellu lar antigens (Avrameas and Uriel, 1966, Lawellin et al, 1977a,b, Nakane, 1975, and Nakane and Pierce, 1967, virus and virus antigen localization and identification in cell and tissue culture(Ubertini et al, 1971, andWicker, 1971), assays for hormones(Weeman et al, 1978), clinical application for the identification of soluble antibodies and antigens…”