Enzyme Immunoassay to Measure Low Levels of Haptoglobin in Biological Fluids

Kaţnik, I; Dobryszycka, W

doi:10.1080/01971529008055047

Cited by 18 publications

(21 citation statements)

References 9 publications

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“…An alternative arrangement was first described by Kątnik and Dobryszycka (1990) which utilizes the ability of Hp to bind Hb. These authors developed an ELISA to detect Hp in human biological fluids.…”

Section: Discussionmentioning

confidence: 99%

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Determination of Haptoglobin in Bovine Serum using Polyclonal and Monoclonal Anti-human Haptoglobin Antibodies

2010

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Two ELISA procedures to determine haptoglobin (Hp) in bovine serum were developed. Equine haemoglobin was used as the solid phase. Self-developed goat polyclonal antibody (variant I) and monoclonal antibody (variant II) raised against human Hp were used. The results were compared with the guaiacol method. High correlation was found (r = 0.96 and r = 0.90, respectively) based on the results of 548 bovine serum samples, of which 357 were from clinically healthy cows and 191 from cows and calves monitored during treatment for the most common diseases. The Hp detection limit of ELISA using polyclonal Ab was 0.1 mg/l and using MoAb 0.21 mg/l. The addition of 2% PEG 6000 at the antibody-binding steps enabled major shortening of the incubation periods. The relatively short time, low cost of reagents, and high correlation with the reference method support the use of these ELISA variants in bovine diagnostics. Cattle, acute-phase protein, enzyme immunoassay, haptoglobin antibody

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Section: Discussionmentioning

confidence: 99%

“…ELISA for human haptoglobin (Kątnik and Dobryszycka 1990) was modified for bovine Hp determination. Two variants of ELISA were conducted.…”

Section: Hp Concentration Measurements Guaiacol Methodsmentioning

confidence: 99%

Determination of Haptoglobin in Bovine Serum using Polyclonal and Monoclonal Anti-human Haptoglobin Antibodies

2010

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“…We also demonstrated some differences in the quantitation of haptoglo in in pathological body fluids, between the "traditional" Hp-Hb method and the method based on Hp-concanavalin A complex formation (7). The purpose of the present study was to investigate whether these reactivities of haptoglobin, as determined in the sera of patients with ovarian tumours, might be used as an index of disease activity.…”

Section: Introductionmentioning

confidence: 99%

Measurements of Haptoglobin by the Reaction with Concanavalin A in Sera of Patients with Ovarian Tumours

Kaţnik¹,

Jadach²,

Kmieciak³

et al. 1995

CCLM

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Summary:The concentration of haptoglobin in sera of healthy women and patients with non-malignant and malignant ovarian tumours was measured by two methods, i.e. complex formation with haemoglobin and complex formation with concanavalin A. High correlation (r = 0.74) between both the methods was found in the group of healthy women, but correlation coefficients were much lower in the group of non-malignant and malignant tumours (r = 0.42 and 0.37, respectively). Direct determinations of haptoglobin, and calculation of the ratio of haptoglobin bound to concanavalin A to haptoglobin bound to haemoglobin revealed statistically significant differences among the examined groups. Comparison of two methods of haptoglobin quantitation suggest that processes connected with ovarian disorders may alter the glycosylation of haptoglobin oligosaccharide chains.

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“…This might be at least partially explained by the difficulties in determination of the acute phase protein in the oral cavity due to their low concentrations (Backhausz, 1975;Grimoud, 1998;Katnik, 1990;Keur, 1994). Some authors reported immunoenzyme method to determine haptoglobin in different biofluids, including saliva, which ranged 5 to 150 μg/l (Katnik, 1990). The salivary haptoglobin measurement is commonly used in the veterinary practice (Hiss, 2003;.…”

Section: Haptoglobinmentioning

confidence: 99%

“…There are a limited number of studies regarding the salivary haptoglobin (Backhausz, 1975;Grimoud, 1998;Katnik, 1990). This might be at least partially explained by the difficulties in determination of the acute phase protein in the oral cavity due to their low concentrations (Backhausz, 1975;Grimoud, 1998;Katnik, 1990;Keur, 1994). Some authors reported immunoenzyme method to determine haptoglobin in different biofluids, including saliva, which ranged 5 to 150 μg/l (Katnik, 1990).…”

Section: Haptoglobinmentioning

confidence: 99%