1997
DOI: 10.1021/ac960995x
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Enzyme Immunoassay Utilizing Surface-Enhanced Raman Scattering of the Enzyme Reaction Product

Abstract: We propose here a new enzyme immunoassay based on surface-enhanced Raman scattering (SERS). In the proposed system, antibody immobilized on a solid substrate reacts with antigen, which binds with another antibody labeled with peroxidase. If this immunocomplex is subjected to reaction with o-phenylenediamine and hydrogen peroxide at 37°C, azoaniline is generated. This azo compound is adsorbed on a silver colloid. In this system, only the azo compound gives a strong surface-enhanced resonance Raman (SERRS) spect… Show more

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Cited by 156 publications
(138 citation statements)
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“…For instance, much work has been done in nucleic acid hybridization assays in which the modified gold nanoparticles are aggregated and then hybridize with target DNA and can be detected by a colorimetric method (3). More recently, surface-enhanced Raman scattering with colloidal silver and gold nanoparticles as enhancing substrates has been developed as a sensitive readout for a heterogeneous immunoassay (6,9,27). Although this technique is attractive for the future development of a multiplex microarray platform by using multilabel techniques (27), the detection strategy is costly and complex, as it requires special surface coating and the use of a laser (9).…”
Section: Discussionmentioning
confidence: 99%
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“…For instance, much work has been done in nucleic acid hybridization assays in which the modified gold nanoparticles are aggregated and then hybridize with target DNA and can be detected by a colorimetric method (3). More recently, surface-enhanced Raman scattering with colloidal silver and gold nanoparticles as enhancing substrates has been developed as a sensitive readout for a heterogeneous immunoassay (6,9,27). Although this technique is attractive for the future development of a multiplex microarray platform by using multilabel techniques (27), the detection strategy is costly and complex, as it requires special surface coating and the use of a laser (9).…”
Section: Discussionmentioning
confidence: 99%
“…Multiple nanoparticle detection methods have been developed, including scanning and transmission electron microscopy (12,25), Raman spectroscopy (6,9,27), and the naked eye (16,24). Detection by the naked eye may be preferable, as other techniques are expensive and require specialized equipment and additional preparations.…”
mentioning
confidence: 99%
“…Cotton et al pioneered the use of NP and SERS for proteomic investigations and developed the first SERSbased enzyme immunoassay in 1989 (29). Subsequently, a number of SERS-based immunoassays have been documented (30)(31)(32)(33)(34)(35)(36)(37)(38)(39). Assembly of functionalized NPs via protein-ligand interactions has been previously demonstrated using techniques such as extinction spectroscopy, transmission electron microscopy, and gel electrophoresis, all of which lack the sensitivity of SERS (40)(41)(42)(43)(44).…”
mentioning
confidence: 99%
“…paratuberculosis at low levels. Several laboratories, including our own, have demonstrated the merits of SERS readout in immunoassays (1,19,20,31,43,45,47,51,67,69) and DNA detection (9,25,29). In SERS, roughened metal surfaces amplify the Raman scattering of an adsorbed organic molecule.…”
mentioning
confidence: 99%