Enzyme-induced modification of the surface properties of lymphoid cells in malignant disease. I. Effect of trypsin on rosette formation by lymphocytes in myelomatosis

Fajumi, James

doi:10.1038/bjc.1980.28

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…The percentage of lymphocytes with Fcy or C3b receptors was found normal in the present study. This is somewhat in contrast to several reports which have found an increase in EAC-R F C percentage (24)(25)(26)(27)(28).…”

Section: Discussioncontrasting

confidence: 99%

Decrease of Fcγ and C3b receptor‐bearing granulocytes and of T lymphocytes in myelomatosis

Naess

Hopen

Solberg

1984

Scandinavian Journal of Haematology

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18 patients with myelomatosis had lower percentages of granulocytes bearing receptors for Fcγ (47.6%) and C3b (43.0%) than controls (80.4% and 75.0%). The percentage of T lymphocytes was decreased in patients when untreated sheep erythrocytes were used as indicator cells. Patients with high serum IgG levels had lower percentages of T lymphocytes. There was no significant difference in receptor profile between treated and untreated patients. The decrease in Fcγ and C3b receptor‐bearing granulocytes in myelomatosis is probably not due to serum or plasma factors since (a) the distribution of receptor‐bearing lymphocytes was not different from that of the controls; (b) extra washings of cells or overnight incubation did not enhance the percentage of receptor positive cells; and (c) incubation of normal granulocytes in sera or plasma from myelomatosis patients did not cause a more pronounced reduction in the proportions of Fcγ of C3b receptor‐bearing cells than incubation in normal sera or plasma.

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Section: Discussioncontrasting

confidence: 99%

Decrease of Fcγ and C3b receptor‐bearing granulocytes and of T lymphocytes in myelomatosis

Naess

Hopen

Solberg

1984

Scandinavian Journal of Haematology

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In situ identification of T6-positive cells in normal human dermis by immunoelectron microscopy*

et al. 1983

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Monoclonal anti-T6 antibody, which reacts with the majority of cortical thymocytes but not peripheral T cells, also reacts with human epidermal Langerhans cells, as shown by a four-step immunoperoxidase method and immunoelectron microscopy. To define whether T6-positive cells are also present in normal human dermis, we used these techniques to demonstrate two immunologically distinct populations of histiocyte-like cells in normal human dermis. The first population contains cells devoid of phagolysosomes or Birbeck granules. These cells react with anti-T6 antibody, but not with monoclonal anti-T3 antibody which defines peripheral T cells, and are found predominantly in and around dermal lymphatic vessels. The second is composed of phagolysosome-containing cells which do not react with anti-T6 antibody or anti-T3 antibody. Because to date, Langerhans cells are the only cells in normal human epidermis that react with anti-T6 antibody, these data provide immunological evidence for a specific link between Langerhans cells and a T6-positive dermal mononuclear cell, possibly the so-called indeterminate cell. In addition, application of these techniques should, for the first time, permit the immunological distinction of these T6-positive mononuclear cells from other cells bearing Ia antigens, such as dermal histiocytes and certain lymphocytes, in normal and diseased skin.

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Effects of trypsin on the in situ identification of epidermal cell membrane antigens

et al. 1987

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In this work the role of trypsin in revealing epidermal cell surface antigens, with the use of immunological markers, was investigated. Two monoclonal antibodies (MCA) were used, the first: D47, belongs to the first cluster of differentiation and recognizes a membrane antigen of human thymocytes; the second HLA-ABC-m3, is an anti-HLA-B27 MCA. Preliminary treatment with various concentrations of trypsin was performed on frozen skin sections and followed by indirect immunofluorescence. D47 reacted with epidermal dendritic cells only after trypsin pretreatment of skin sections. In addition a mild preliminary trypsinization was shown to increase in situ immunoreactivity of MCA HLA-ABC-m3 with epidermal cells. Best results were obtained when trypsin concentrations ranging from 2.5 to 5 micrograms/ml were applied for 10 min at 37 degrees C. Preliminary trypsinization may be of interest for a better exposure of some surface antigens to immunohistochemical markers.

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Enzyme-induced modification of the surface properties of lymphoid cells in malignant disease. I. Effect of trypsin on rosette formation by lymphocytes in myelomatosis

Cited by 3 publications

References 10 publications

Decrease of Fcγ and C3b receptor‐bearing granulocytes and of T lymphocytes in myelomatosis

Decrease of Fcγ and C3b receptor‐bearing granulocytes and of T lymphocytes in myelomatosis

In situ identification of T6-positive cells in normal human dermis by immunoelectron microscopy*

Effects of trypsin on the in situ identification of epidermal cell membrane antigens

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