Enzyme induction in soybean infected by Phytophthora megasperma f.sp. glycinea

Börner, H.; Grisebach, Hans

doi:10.1016/0003-9861(82)90479-9

Cited by 57 publications

(12 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The induction of glucose-6-P dehydrogenase was about as rapid as the induction of PAL and 4CL, and a similar observation has been made with Pmg-infected soybean hypocotyls (5). A rapid increase in this enzyme activity, which controls the flux through the oxidative pentose phosphate pathway, might therefore be directly related to the plant's defense response, reflecting an increased need for certain metabolites, such as ATP, NADPH, and ribulose 5-P.…”

Section: Methodssupporting

confidence: 68%

Responses of Cultured Parsley Cells to Elicitors from Phytopathogenic Fungi

Kombrink¹,

Hahlbrock²

1986

Plant Physiol.

165

View full text Add to dashboard Cite

Cultured parsley cells (Petroselinum crispum) responded to treatment with heat-released soluble cell-wall fragments (elicitors) from several different phytopathogenic fungi by forming coumarin derivatives (phytoalexins). This response was preceded in all cases by large but transient increases in the activities of two enzymes of general phenylpropanoid metabolism, phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL). The activities of two hydrolytic enzymes, chitinase and 1,3-Bl-glucanase, also increased strongly in elicitor-treated cells, whereas the activities of three enzymes participating in primary metabolism were affected differently by the elicitor treatment. Glucose-6-phosphate dehydrogenase increased, phosphofructokinase remained almost constant, and pyrophosphate:fructose-6-phosphate phosphotransferase declined sharply in activity. Different amounts of cell-wall preparations from various phytopathogenic fungi were required for maximum elicitor activity. While three oomycetes (Phytophthora spp.) yielded the most active elicitors studied (maximum coumarin accumulation at concentrations of about 10 microgram per milliliter), cell-wall preparations from an ascomycete and three deuteromycetes gave comparable results only at 10 to 100 times higher concentrations. Optimal induction of PAL, 4CL, and chitinase with Phytophthora elicitor required only about 1 microgram per milliliter, whereas 1,3-1-glucanase induction showed a dose dependence similar to that observed for coumarins. The elicitor concentration had pronounced effects not only on the extent, but also on the timing of all induced reactions.

show abstract

Section: Methodssupporting

confidence: 68%

Responses of Cultured Parsley Cells to Elicitors from Phytopathogenic Fungi

Kombrink¹,

Hahlbrock²

1986

Plant Physiol.

165

View full text Add to dashboard Cite

show abstract

“…6). This delay is in contrast to the rapid induction of other phenylpropanoid pathway enzymes (less than 24 h) by wounding or infection (2,25 ( 14). Peroxidase has a crucial role in the final step in this process and the understanding of peroxidase expression will open possibilities in crop improvement through the development of stress-tolerant plants.…”

Section: Anionic Peroxidase Kineticsmentioning

confidence: 93%

Wound-Induced Deposition of Polyphenols in Transgenic Plants Overexpressing Peroxidase

1991

View full text Add to dashboard Cite

“…In particular, marked increases in phenylalanine ammontia-lyase synthesis and translatable mRNA activity have been observed in response to elicitor treatment or infection in a number of systems (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21). Thus, treatment of suspension-cultured bean (Phaseolus vulgaris L.) cells with an elicitor heat-released from cell walls of the fungus Colletotrichum lindemuthianum causes a rapid increase in the rate of synthesis of phenylalanine ammonialyase and at least three other enzymes of phenylpropanoid biosynthesis associated with the accumulation of wall-bound phenolic material and isoflavonoid phytoalexins such as phaseollin (refs.…”

Section: Introductionmentioning

confidence: 99%