Enzyme Inhibition (AChE) in Brain of Oreochromis mossambicus due to Pesticidal Pollution of Herbicide “Pursuit”

Pasha, Farhina

doi:10.21608/eajbsc.2013.16114

Cited by 5 publications

(3 citation statements)

References 4 publications

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“…Analyzing the biochemical aspect, IMZT is also capable of inducing alterations in AChE activity in L. latinasus adults. In this sense, our results corroborate the same effects reported in sh by Pasha and Singh (2005), Moraes et al (2011) and Pasha (2013), where IMZT alters the cholinergic system of local aquatic vertebrates. Oxidative stress refers to the imbalance due to excess ROS or oxidants over the cell's ability to carry out an effective antioxidant response.…”

Section: Discussionsupporting

confidence: 91%

Multiple Level Effects of Imazethapyr on Leptodactylus latinasus (Anura) Adult Frogs

Iglesias

Fanali

Franco‐Belussi

et al. 2021

Arch Environ Contam Toxicol

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Imazetapir is a herbicide used in soybean and corn crops worldwide. Ecotoxicological studies have shown that imazetapir promotes genotoxic, biochemical and individual effects in aquatic vertebrates. In this study, we evaluated the response of different biomarkers in adult specimens of Leptodactylus latinasus exposed under laboratory conditions to the imazetapir based-formulation Pivot® H (10.59% Imazetapir) mimicking two possible real acute scenarios. Both exposure scenarios considered were the runoff simulation (scenario1: 10 mg/L) and the direct spraying application (scenario2: 1000 mg/L).Different endpoints were evaluated at several ecotoxicological levels after 48 and 96 h of exposure including individual (biometric indices and behavior alterations), histological (liver pigmentation and tissue alterations), biochemical (catalase, glutathione system and cholinesterase activities) and genotoxic effects (induction of micronuclei and nuclear abnormalities). The exposure to Pivot® H during 48 h, induced inhibition of the glutathione-S-transferase activity in scenario1 and an increase of hepatic tissue alterations and acetyl-cholinesterase levels in scenario2. After 96 h, we demonstrated that imazetapir formulation induced a decrease in melanin and hemosiderin, an increase in catalase activity and induction of micronuclei in scenario1 while in scenario2 there was a decrease in the hepatosomatic index, and an increase in liver alterations and melanin reduction. The multivariate analysis allows to correlate biomarkers at the same level in exposed specimens. Accordingly, we conclude that populations of L. latinasus could be at risk after real scenarios of exposure to pesticides corroborating that the species is a good model for ecotoxicological studies in the region.

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Section: Discussionsupporting

confidence: 91%

Multiple Level Effects of Imazethapyr on Leptodactylus latinasus (Anura) Adult Frogs

Iglesias

Fanali

Franco‐Belussi

et al. 2021

Arch Environ Contam Toxicol

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“…No obstante, se reportó que la exposición a IMZT como principio activo y en formulados comerciales, produce estrés oxidativo en células hepáticas de Cyprinus carpio (carpa común) al evaluar distintos parámetros bioquímicos como la respuesta de las enzimas de estrés oxidativo y enzimas colinesterasas (Moraes y col. 2011). Estos efectos también fueron reportados para O. mossambicus (tilapia de Mozambique) luego de la exposición a formulados comerciales (Pasha y Singh 2005, Pasha 2013. De cualquier manera, hasta el momento para otros vertebrados acuáticos como los anfibios, no se tiene registros sobre los posibles efectos adversos de IMZT.…”

Section: Herbicida Imazetapir: Características Toxicidad Y Genotoxicunclassified

Are the damaging effects induced by the imazethapyr formulation Pivot® H in Boana pulchella (Anura) reversible upon ceasing exposure?

Iglesias

Natale

Soloneski

et al. 2018

Ecotoxicology and Environmental Safety

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In the present study, the damage recovery capabilities of Boana pulchella tadpoles after acute exposure (96h) to 0.39mg/L concentration of the imazethapyr (IMZT)-based herbicide formulation Pivot H (25% IMZT LC value) were assessed during a period of 7 to -21 days. To appraise the recovery capabilities, frequency of micronuclei (MNs), other nuclear abnormalities and DNA single-strand breaks evaluated by single cell gel electrophoresis assay on circulating blood cells were employed as endpoints for genotoxicity. Growth, development, body mass, and morphological abnormalities were also employed as individual endpoints in the recovery assay. Results demonstrated that IMZT induced sublethal effects at both the individual (i.e., loss of keratodonts) and cytogenetic levels (e.g., increase of MN frequency, other nuclear abnormalities and DNA single-strand breaks). At 11 days of the exposure phase, tadpoles recovered their basal levels of frequency of MNs, other nuclear abnormalities, and comets. However, loss of keratodonts, observed at the end of the exposure period, was present up to 21 days thereafter. Finally, axial abnormalities and delay in development stage were observed only during the postexposure phase in IMZT-exposed tadpoles at 18 and 25 days, respectively and were observed until the end of the experiment. This is the first evidence of use the comet assay as cytogenetic biomarker of genotoxicity in evaluating the recovery capabilities of amphibians in general and also those of B. pulchella after exposure to IMZT.

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“…Furthermore, analyzing in aquatic vertebrates, Moraes et al (2011) reported alterations on enzymes related to oxidative stress after 0.0148 mg/L exposure to both the active ingredient IMZT and to the IMZT-based commercial herbicide formulation Only® in hepatic tissues of the sh Cyprinus carpio. In addition, inhibition of acetylcholinesterase (AChE) was informed after exposure to commercial formulations of IMZT in the Mozambique tilapia (Pasha and Singh, 2005;Pasha, 2013). Recently, in other aquatic vertebrates, our research group has demonstrated the toxicological effects at genetic, biochemical and individual level in anurans such as Boana pulchella and Leptodactylus latinasus exposed to IMZT at their larval phase (Pérez-Iglesias et al, 2015;2017;2020).…”

Section: Introductionmentioning

confidence: 99%

Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs

et al. 2023

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Imazethapyr, a post-emergent herbicide used in worldwide soybean and corn crops, induces genetic and biochemical alterations in aquatic vertebrates. This study examined relationship between biomarkers at different organization levels and imazethapyr real-life route exposure in Boana pulchella adults. Frogs were exposed to imazethapyr-based formulation Pivot® H (10.59%) at concentrations representing possible acute routes: eld runoff (S1:10 mg.L − 1 ), direct foliar application with posterior frog presence (S2:100 mg.L − 1 ) and with frog presence (S3:1000 mg.L − 1 ). Post-exposure, endpoints levels were evaluated: organism alterations, biochemical activities and cytogenetic assays. Forty-eight hours postexposure, antioxidant enzymes decrease, micronuclei induction and DNA damage were observed in all scenarios; while cholinesterase activity increase and body condition reduction were observed in frogexposed to S3. Ninety-six hours post-exposure, frogs showed glutathione-S-transferase inhibition in S1, micronuclei induction in S2 and S3, and DNA-damage increase in S3. Herbicides routes of exposures in real-life could indicate that authorized applications have a risk to frogs populations.

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Enzyme Inhibition (AChE) in Brain of Oreochromis mossambicus due to Pesticidal Pollution of Herbicide “Pursuit”

Cited by 5 publications

References 4 publications

Multiple Level Effects of Imazethapyr on Leptodactylus latinasus (Anura) Adult Frogs

Multiple Level Effects of Imazethapyr on Leptodactylus latinasus (Anura) Adult Frogs

Are the damaging effects induced by the imazethapyr formulation Pivot® H in Boana pulchella (Anura) reversible upon ceasing exposure?

Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs

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