Enzyme inhibitory assessment of the isolated constituents fromPlantago holosteumScop.

Abstract

Plants of the Plantago genus are widely used in Turkish folk medicine especially for the treatment of wound, abscess, and inflammation. The aqueous extract and five phenylethanoid glycosides acteoside (1), arenarioside (2), echinacoside (3), isoacteoside (4), and leucosceptoside A (5) isolated from the aerial parts and roots of Plantago holosteum Scop. (Plantaginaceae) were tested for their possible inhibitory activity against hyaluronidase, elastase, and collagenase, related to wound pathogenesis. Even though the aqueous extract prepared from the aerial parts (36.26%) and roots (47.01%) and the isolated compounds acteoside (29.13%), echinacoside (28.73%), and isoacteoside (31.69%) exerted a notable inhibition, arenarioside and leucosceptoside A were found inactive in the hyaluronidase enzyme inhibition test. Similar results were obtained from the collagenase enzyme inhibition test. The aqueous extract prepared from the aerial parts (31.09%) and roots (35.17%), echinacoside (25.13%), and isoacteoside (23.85%) exerted a notable inhibition in this test. However, none of the extracts and isolated compounds displayed elastase enzyme inhibitory activity. The experimental data demonstrated that P. holosteum displayed a remarkable enzyme inhibitory activity against hyaluronidase and collagenase. This paper is the first report regarding the in vitro enzyme inhibitory activity of P. holosteum.

The Wound‐Healing Potential of the Endemic Plant Helianthemum canum (L.) Baumg: Preclinical Studies Supported with Phytochemical Profiling

“…The mixture was heated and held in a water bath after adding 50 μL of 0.4 M NaOH and 100 μL of 0.2 M sodium borate. After cooling, 1.5 mL of p‐dimethylamino benzaldehyde was added and incubated at 37 °C for 20 minutes, then the absorbance was measured at 585 nm [39,40] …”

“…After cooling, 1.5 mL of p-dimethylamino benzaldehyde was added and incubated at 37 °C for 20 minutes, then the absorbance was measured at 585 nm. [39,40] In vivo wound healing activity…”

Isolation of Some Phenolic Compounds from Plantago subulata L. and Determination of Their Antidiabetic, Anticholinesterase, Antiepileptic and Antioxidant Activity

“…The extract was suspended in distilled water and partitioned with petroleum ether to remove nonpolar compounds such as chlorophylls. The aqueous phase was evaporated and lyophilized to give 59.4 g of the aqueous extract and used for the phytochemical analyses by different chromatographic techniques according to previous studies [40,41] …”

“…The aqueous phase was evaporated and lyophilized to give 59.4 g of the aqueous extract and used for the phytochemical analyses by different chromatographic techniques according to previous studies. [40,41]…”