Enzyme Initiated Radical Polymerizations

Hollmann, Frank; Arends, Isabel W. C. E.

doi:10.3390/polym4010759

Cited by 193 publications

(151 citation statements)

References 204 publications

(195 reference statements)

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“…The pH change in this case too was negligible, suggesting the possible oxidation of BB molecules in the course of decolourisation. On oxidation, the BB molecules in the solution seem to polymerise as reported for phenols and anilines (Hollmann and Arends 2012). The polymerised dye tends to form a precipitate, as reported in case of phenols (Nicelle et al 1995).…”

Section: Discussionmentioning

confidence: 62%

“…The H 2 O 2 requirement of MO is lower than that of BB since it appears to be more easily oxidised than BB. At higher concentrations of H 2 O 2 , the decolourisation of MO is lower since the crude enzyme may have become inactivated (Veitch 2004;Hollmann and Arends 2012) when the substrate is MO. It has been indicated in the graph that there is some decolourisation even in the absence of added H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

“…These polymers grow in length by the addition of newly oxidised dye radicals. The large size of the polymerised dye and its poor solubility in aqueous solutions cause precipitation (Hollmann and Arends 2012). The pH of the reaction mixture would be one of the factors governing the susceptibility of BB molecules to enzymatic oxidation.…”

Section: Discussionmentioning

confidence: 99%

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Enzymatic decolourisation of Methyl Orange and Bismarck Brown using crude peroxidase from Armoracia rusticana

Ambatkar¹,

Mukundan²

2014

Appl Water Sci

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The decolourisation of Methyl Orange (MO) and Bismarck Brown (BB) by crude peroxidase from Armoracia rusticana (Horseradish) was studied by varying different reaction parameters. The pH of the reaction mixture, initial dye concentration, amount of enzyme and hydrogen peroxide concentration were optimised for ambient temperatures (30 ± 2°C). The optimum pH for decolourisation was 4.0 (72.95 %) and 3.0 (79.24 %) for MO and BB, respectively. Also it was found that the Chemical Oxygen Demand of the enzyme-treated sample was significantly lower than that of the untreated controls for both dyes. The addition of a complex iron salt like Ferric EDTA was found to enhance the decolourisation of both dyes at pH 6.0, showing an increase of 8.69 % and 14.17 % in the decolourisation of MO and of BB, respectively. The present study explores the potential of crude peroxidase from horseradish to decolourise representative monoazo and diazo dyes, MO and BB, respectively. An attempt has been made to utilise a crude enzyme with appreciable activity obtained after minimal processing for the decolourisation of the aforesaid dyes. The findings of this study would find application in the enzymatic treatment of wastewater containing azo dyes.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

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Enzymatic decolourisation of Methyl Orange and Bismarck Brown using crude peroxidase from Armoracia rusticana

Ambatkar¹,

Mukundan²

2014

Appl Water Sci

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“…Outras enzimas como oxidases (EC classe 1) e lipoxigenases (EC 1.13.11.x) exercem um papel menor nessas reações. 88 As peroxidases, especialmente as proteínas do grupo Heme como a peroxidase de raiz forte (horseradish peroxidase, EC 1.11.1.7), têm sido umas das mais estudadas como catalisadoras da polimerização via radicais livres de monômeros vinila e compostos aromáticos. Elas podem catalisar a transferência de um elétron de um peróxido de hidrogênio para um cossubstrato, criando radicais de forma efetiva e, assim, iniciando a polimerização.…”

Section: Polifenóis E Polimerização Via Radicais Livresunclassified

Applications of Enzymes in Synthesis and Modification of Polymers

Albuquerque¹,

Ribeiro²,

Rabelo³

et al. 2014

Química Nova

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Recebido em 07/06/2013; aceito em 10/12/2013; publicado na web em 21/03/2014 APPLICATIONS OF ENZYMES IN SYNTHESIS AND MODIFICATION OF POLYMERS. Enzymes are biological catalysts that offer great potential for use in the synthesis and modification of polymers, being more specific and greener than chemical catalysts. In this work, enzymes from the classes of hydrolases (lipase, cutinase and protease) and of oxidoreductases (horseradish peroxidase, manganese peroxidase and laccase) were identified as the main biocatalysts responsible for the synthesis of polymers. Biocatalysis can potentially be part of the life cycle of several polymers, including polyesters, polyurethanes, polycarbonates, polyamides, functionalized polysaccharides and polystyrene, allowing the synthesis of specialty macromolecules for fine applications and with higher added-value than commodity polymers.Keywords: enzyme polymerization; biocatalysis; lipase. INTRODUÇÃOProcessos de polimerização representam uma das principais transformações químicas industriais da atualidade, sendo, em sua maioria, conduzidos na presença de catalisadores químicos não renováveis e sob elevadas temperaturas, demandando, em alguns casos, a proteção e desproteção de grupos funcionais, de forma a evitar a ocorrência de reações indesejadas. 1 Há cerca de 20 anos, a catálise enzimática tem sido apontada como uma estratégia de grande potencial para a síntese de políme-ros, por ser ambientalmente amigável e em geral bastante seletiva, permitindo assim a obtenção de macromoléculas com propriedades diferenciadas e de maior valor agregado.2 As enzimas para a síntese e também modificação de polímeros estão nas classes das hidrolases (como lipases, proteases e esterases) e das oxidorredutases. 3A biocatálise pode ser empregada tanto para a síntese de polí-meros naturais como de polímeros sintéticos, e dentre suas maiores vantagens está a elevada seletividade (enantio-, quimio-, éstereo-e régio-seletividade), levando à obtenção de polímeros perfeitamente estruturados na maioria dos casos. Além disso, a catálise enzimática ocorre em geral de forma bastante eficiente sob condições mais brandas de temperatura e pressão, 4 demanda reduzida (ou nenhuma) quantidade de solventes orgânicos, 5 não proporciona a formação de produtos secundários e os produtos finais são de fácil separação e recuperação.6-8 Tais vantagens ficam claras especialmente quando são empregados biocatalisadores na forma imobilizada, a qual é uma técnica amplamente reportada pela literatura.9,10 A biocatálise foi reconhecida na década de 1990 como uma área nova na síntese de polímeros de precisão. 11Dessa forma, o objetivo desse artigo é apresentar um panorama das principais enzimas e condições de processo que podem ser utilizadas para a síntese e a modificação de polímeros de interesse industrial. O reconhecimento da importância do tema se traduz pela existência hoje de livros inteiramente dedicados à polimerização enzimática. 12-14 APLICAÇÕES DE ENZIMAS NA SÍNTESE DE POLÍMEROS PoliésteresOs poliésteres estão dentre ...

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“…Roots of this plant are rich in peroxidase, heme containing enzyme belonging to oxidoreductases (EC 1.11.1.7). Applications of horseradish peroxidase (HRP) as noted Hollmann and Arends [1] include removal of peroxide, phenols, amines, indols and other heterocyclic compounds from materials such as food stuffs and industrial waste, as oil refineries, plastics, resins, textiles, iron and steel, forestry industries waste water containing phenolic compounds, synthesis of various aromatic chemicals, in biocatalysis and in radical polymerization. Veitch [2] have reported the decolorization and removal of textile dyes from polluted water and dyeing effluents by using soluble and immobilized peroxidases.…”

Section: Introductionmentioning

confidence: 99%

Biocatalytic properties of horseradish root extract peroxidase (HRP)

Purev¹,

J²,

Tsevelmaa³

et al. 2014

Mong. J. Chem.

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Abstract:The objective of the present work was to examine biocatalytic properties of peroxidase in horseradish acclimatized in our country. We have found that horseradish root extract's peroxidase (HRP) has K M 2.5 mM and V max 5.36 mM·s -1 . Maximum activity (pH opt ) was estimated at pH 6.0 and enzyme is more stable in alkali, than in acid. The optimum temperature (T opt ) for HRP is 40 o C and the enzyme is not stable to temperature influence. The horseradish root's extract retains enzymatic activity within 21 days.

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Enzyme Initiated Radical Polymerizations

Cited by 193 publications

References 204 publications

Enzymatic decolourisation of Methyl Orange and Bismarck Brown using crude peroxidase from Armoracia rusticana

Enzymatic decolourisation of Methyl Orange and Bismarck Brown using crude peroxidase from Armoracia rusticana

Applications of Enzymes in Synthesis and Modification of Polymers

Biocatalytic properties of horseradish root extract peroxidase (HRP)

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