Enzyme-linked bio-immunoassay for IFN-γ by HLA-DR induction

Gibson, Ursula E. M.; Kramer, Susan M.

doi:10.1016/0022-1759(89)90083-5

Cited by 25 publications

(6 citation statements)

References 18 publications

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“…The MHC class I1 antigen has been reported to play an important role in control of the growth of human tumors and the glycoprotein, HLA-DR, a member of this group, acts in antigen presentation for the induced lymphoid reaction involving cytotoxic T cells and other immunocompetent cells. IFNy, which is produced during T lymphocyte stimulation, induces the expression of HLA class I1 molecules [26], suggesting that their positive expression might facilitate the regulation or suppression of tumor growth through various lymphocyte responses. Furthermore, Gutierrez et al [5] have indicated that HLA-DR(-) cells are unable to stimulate helper T lymphocytes and thus might escape immunologic control.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemically demonstrated expression of HLA‐DR antigen in colorectal adenocarcinomas and its relation to clinicopathological features

Morita¹,

Tanaka²,

Kuroda³

et al. 1995

Journal of Surgical Oncology

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We studied 148 colorectal adenocarcinomas to clarify any correlation between HLA-DR antigen expression on tumor cells and histopathological features. Paraffin sections of formalin-fixed tissues were stained with HLA-DR antigen using the indirect immunoperoxidase technique. All the tumor tissues were divided into two groups, depending on the incidence of HLA-DR-positive cells (greater and lesser than 50%). Carcinoma tissues with a higher incidence showed less mural invasion, lymphoductal invasion, venous invasion, lymphonodular metastasis, and peritoneal metastasis. Tissues with a high HLA-DR reactivity were more often observed for Dukes' A and B stages, whereas those with a low HLA-DR positivity were frequently Dukes' C and D stages. As for the cumulative survival rate, the group with high HLA-DR expression demonstrated significantly better survival. We speculate that HLA-DR expression by colorectal cancer cells exerts a favorable influence on clinical course.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemically demonstrated expression of HLA‐DR antigen in colorectal adenocarcinomas and its relation to clinicopathological features

Morita¹,

Tanaka²,

Kuroda³

et al. 1995

Journal of Surgical Oncology

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“…An IFN␥-induced HLA-DR antigen was measured with an enzyme-linked bio-immunoassay (31). For that purpose, Colo 205 cells seeded in a 96-well microtiter plate were treated with IFN␥ during a 4-h period.…”

Section: Methodsmentioning

confidence: 99%

Heparan Sulfate Mimicry

Sarrazin

Bonnaffé

Lubineau

et al. 2005

Journal of Biological Chemistry

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Cell-associated heparan sulfate (HS) is endowed with the remarkable ability to bind numerous proteins. As such, it represents a unique system that integrates signaling from circulating ligands with cellular receptors. This polysaccharide is extraordinary complex, and examples that define the structure-function relationship of HS are limited. In particular, it remains difficult to understand the structures by which HS interact with proteins. Among them, interferon-␥ (IFN␥), a dimeric cytokine, binds to a complex oligosaccharide motif encompassing a N-acetylated glucosamine-rich domain and two highly sulfated sequences, each of which binds to one IFN␥ monomer. Based on this template, we have synthesized a set of glycoconjugate mimetics and evaluated their ability to interact with IFN␥. One of these molecules, composed of two authentic N-sulfated octasaccharides linked to each other through a 50-Å-long spacer termed 2O 10 , displays high affinity for the cytokine and inhibits IFN␥-HS binding with an IC 50 of 35-40 nM. Interestingly, this molecule also inhibits the binding of IFN␥ to its cellular receptor. Thus, in addition to its ability to delocalize the cytokine from cell surface-associated HS, this compound has direct anti-IFN␥ activity. Altogether, our results represent the first synthetic HS-like molecule that targets a cytokine, strongly validating the HS structural determinants for IFN␥ recognition, providing a new strategy to inhibit IFN␥ in a number of diseases in which the cytokine has been identified as a target, and reinforcing the view that it is possible to create "tailor-made" sequences based on the HS template to isolate therapeutic activities. Current research increasingly implicates heparan sulfate (HS),2 a highly sulfated glycosaminoglycan present in the extracellular matrix and at the cell surface, in a plethora of phenomena that include cell proliferation, cell adhesion, matrix assembly, chemoattraction, inflammation, immune response, development, lipid metabolism, angiogenesis, wound healing, and viral attachment (1, 2). Mechanistically, this extensive functional repertoire often relies on the ability of HS to recognize diverse proteins, the conformation, stability, local concentration, or biological activities of which are modified by the interaction (3-7).Consistent with its wide protein binding activity, HS is structurally complex. It is composed of strongly anionic domains enriched in N-sulfated glucosamines and iduronic acids, typically 3-8 disaccharides long (referred to as NS or heparin-like domains), that bear a variable number of O-sulfate moieties and are hypervariable in sequence. These domains are separated by relatively regular regions encompassing a larger area that contain predominantly N-acetylated glucosamine and glucuronic acid domains (NA domains) and mixed NA/NS regions that make the transition between NA and NS domains (8). It has been thought that specific information for protein recognition resided within the NS domains in HS and, indeed, a large number of "heparin-bindi...

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“…IFN was assayed by its ability to up-regulate MHC class II expression (HLA-DR) on the adenocarcinoma cell line, Colo 205 (Gibson & Kramer, 1989). IFN was assayed by its ability to up-regulate MHC class II expression (HLA-DR) on the adenocarcinoma cell line, Colo 205 (Gibson & Kramer, 1989).…”

Section: Cytokine Assaysmentioning

confidence: 99%

“…IFN bioassay. IFN was assayed by its ability to up-regulate MHC class II expression (HLA-DR) on the adenocarcinoma cell line, Colo 205 (Gibson & Kramer, 1989). For this, a dilution series of samples or IFN standard (88/606 which has been calibrated against the 1st International Standard for IFN, Gg23-901-530) was prepared in 100 l volumes in 96-well microtitre plates and incubated with 100 l volumes of 2 2 10 5 cells/ml for 48 h at 378C.…”

Section: Cytokine Assaysmentioning

confidence: 99%

Cytokine levels in platelet concentrates: quantitation by bioassays and immunoassays

Wadhwa

Seghatchian²,

Lubenko³

et al. 1996

Br J Haematol

104

119

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Some adverse reactions to the transfusion of platelet concentrates (PCs) cannot be attributed to antibodies against blood cells or to subclinical microbial agents. It has been suggested that leucocyte-derived inflammatory cytokines such as interleukin (IL)-1, IL-6 and tumour necrosis factor (TNF) may contribute to a larger number of unexplained non-antibody-mediated adverse reactions. Three types of PCs, containing different levels of leucocytes, are currently produced. Filtration is used on demand to further reduce leucocyte contamination of these components. we have monitored the plasma of PCs prepared by the platelet-rich plasma method (PRP), the buffy-coat method or by apheresis for IL-6, IL-1, transforming growth factor-beta (TGF-beta), TNF and interferon gamma (IFN gamma). Biologically active IL-6 increased in stored PRP-PCs from a mean of 140 pg/ml on day 1 to 2395 pg/ml on day 5/6. Elevated levels of IL-8, as detected by immunoassay, were evident in PRP-PCs during routine storage under blood bank conditions. Small amounts of immunoreactive IL-1 with only minimal biological activity were present in some PRP-PCs by day 5/6. No significant increase in the levels of IL-8, IL-6 or IL-1 were seen in buffy-coat PCs during storage for 5/6 d. For apheresis PCs, an increase in IL-8 content, but not in IL-6 over 6 d was observed. In all three types of PCs, elevated amounts of both bioactive and immunoreactive TGF beta were present, but there was no evidence of any biologically active or immunoreactive TNF alpha. Pre-storage filtration of PRP-PCs for depletion of leucocytes prevented the increase in IL-8 and IL-6 levels of these PCs. Our results show that leucocyte reduction by buffy-coat method reduces cytokine levels to a comparable level to filtered or apheresis PCs, containing low levels of leucocytes, but use of these PCs in minimizing the severity and incidence of reactions in recipients will require clinical evaluation. This is the first comprehensive and comparative study which, on the basis of biological activity of cytokines, directly indicates that the mode of platelet production grossly influences the levels of cytokines.

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Enzyme-linked bio-immunoassay for IFN-γ by HLA-DR induction

Cited by 25 publications

References 18 publications

Immunohistochemically demonstrated expression of HLA‐DR antigen in colorectal adenocarcinomas and its relation to clinicopathological features

Immunohistochemically demonstrated expression of HLA‐DR antigen in colorectal adenocarcinomas and its relation to clinicopathological features

Heparan Sulfate Mimicry

Cytokine levels in platelet concentrates: quantitation by bioassays and immunoassays

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