Enzyme-Linked Immunosorbent Assay (ELISA) Technique for Food Analysis

Sengupta, Pallav; Wang, Chee Woon; Feei, Zheng

doi:10.1007/978-3-030-68636-9_5

Cited by 6 publications

(3 citation statements)

References 104 publications

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“…ELISA is a basic test for the qualitative and quantitative determination of specific epitopes based on the number of antigen–antibody bonds formed. By comparing these bonds to standards, it is possible to estimate their quantity [ 67 , 68 ]. AGPs can also be analysed qualitatively and quantitatively using ELISA.…”

Section: Immunocytochemical Methods For Qualitative and Quantitative ...mentioning

confidence: 99%

A practical guide to in situ and ex situ characterisation of arabinogalactan proteins (AGPs) in fruits

Kutyrieva-Nowak,

Leszczuk,

Zdunek

2023

Plant Methods

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Background Arabinogalactan proteins (AGPs) are plant cell components found in the extracellular matrix that play crucial roles in fruit growth and development. AGPs demonstrate structural diversity due to the presence of a protein domain and an expanded carbohydrate moiety. Considering their molecular structure, the modification of glycosylation is a primary factor contributing to the functional variety of AGPs. Main body Immunocytochemical methods are used for qualitative and quantitative analyses of AGPs in fruit tissues. These include in situ techniques such as immunofluorescence and immunogold labelling for visualising AGP distribution at different cellular levels and ex situ methods such as Western blotting and enzyme-linked immunoenzymatic assays (ELISA) for molecular characterisation and quantitative detection of isolated AGPs. The presented techniques were modified by considering the structure of AGPs and the changes that occur in fruit tissues during the development and ripening processes. These methods are based on antibodies that recognise carbohydrate chains, which are the only commercially available highly AGP-specific tools. These probes recognise AGP epitopes and identify structural modifications and changes in spatio-temporal distribution, shedding light on their functions in fruit. Conclusion This paper provides a concise overview of AGP research methods, emphasising their use in fruit tissue analysis and demonstrating the accessibility gaps in other tools used in such research (e.g. antibodies against protein moieties). It underscores fruit tissue as a valuable source of AGPs and emphasises the potential for future research to understand of AGP synthesis, degradation, and their roles in various physiological processes. Moreover, the application of advanced probes for AGP visualisation is a milestone in obtaining more detailed insights into the localisation and function of these proteins within fruit.

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Section: Immunocytochemical Methods For Qualitative and Quantitative ...mentioning

confidence: 99%

A practical guide to in situ and ex situ characterisation of arabinogalactan proteins (AGPs) in fruits

Kutyrieva-Nowak,

Leszczuk,

Zdunek

2023

Plant Methods

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“…Polymerase chain reaction (PCR) as a DNA-based method (Chung, 2017), restriction fragment length polymorphism analysis (RFLP) (Chen et al, 2010), and DNA barcode markers (Fernandes et al, 2021) also have been used to detect meat fraud. Sengupta et al (2021) reviewed immunological approaches such as ELISA to assess meat products. The most popular approach for determining meat product adulteration is PCR, whereas a method for determining meat products based on their protein composition is proteomic.…”

Section: Introductionmentioning

confidence: 99%

Possibility of using fatty acid profiles for the authentication of beef adulterated with pork, donkey, and dog meat

Elsabagh,

Abdeen,

K. Morsy

et al. 2024

Ital. J. Food Sci.

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Detection of meat adulteration is a critical issue in food labeling procedures and a serious concern related to food fraud, authenticity, and religious beliefs. The current work detected and quantified adulteration of raw ground beef with pork, donkey, and dog meat based on fatty acid profiles using GC-MS/MS. The study design incorporated pork, donkey, or dog meat with beef meat; negative and positive controls were used for the different meat species. Results demonstrated several significant differences (p < 0.05) in fatty acid contents between mixed/adulterated meat and pure beef. In addition, higher total saturated fatty acid levels in ground beef (57.91%) compared to dog fat (46.44%), donkey (38.71%), and pork fat (lard) (40.23%). High total unsaturated fatty acids content was observed in donkey (61.92%), pork (59.77%), and dog (53.56%) fats compared to beef fat (42.09%). On the other hand, total unsaturated and monounsaturated fatty acids in beef meat were lower than in pork, donkey, and dog meat. Moreover, the highest trans-fatty acid content was found in pork compared to the other meat types. All incorporated samples correlated positively with pure ground beef concerning the fatty acid profiles. Therefore, alteration of the mixed beef fatty acid profiles was a potential indicator for adulteration since a marked decrease in total saturated fatty acid content and an increase in unsaturated fatty acids was observed in the substituted samples. We concluded that GC-MS/MS-based fatty acid profiling is a promising technique that can be used to detect meat adulteration.

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“…In many cases, innovative approaches based on well-established instrumental techniques were reported, such as chromatography, electrophoresis, molecular spectroscopy, electrochemistry, and atomic spectroscopy [2,3]. Recently, immunoassays have been successfully commercialized and widely employed in the detection and quantification of prohibited or restricted ingredients in the food industry [6,7]. Considering the increasing danger related to cosmetic safety, the immunological techniques could be an interesting, as well as easier and faster, alternative to provide supervision and control with regard to the presence of harmful additives or species, which would be appropriate for a large number of samples.…”

Section: Introductionmentioning

confidence: 99%

Immunological Analytical Techniques for Cosmetics Quality Control and Process Monitoring

et al. 2021

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Cosmetics analysis represents a rapidly expanding field of analytical chemistry as new cosmetic formulations are increasingly in demand on the market and the ingredients required for their production are constantly evolving. Each country applies strict legislation regarding substances in the final product that must be prohibited or regulated. To verify the compliance of cosmetics with current regulations, official analytical methods are available to reveal and quantitatively determine the analytes of interest. However, since ingredients, and the lists of regulated/prohibited substances, rapidly change, dedicated analytical methods must be developed ad hoc to fulfill the new requirements. Research focuses on finding innovative techniques that allow a rapid, inexpensive, and sensitive detection of the target analytes in cosmetics. Among the different methods proposed, immunological techniques are gaining interest, as they make it possible to carry out low-cost analyses on raw materials and finished products in a relatively short time. Indeed, immunoassays are based on the specific and selective antibody/antigen reaction, and they have been extensively applied for clinical diagnostic, alimentary quality control and environmental security purposes, and even for routine analysis. Since the complexity and variability of the matrices, as well as the great variety of compounds present in cosmetics, are analogous with those from food sources, immunological methods could also be applied successfully in this field. Indeed, this would provide a valid approach for the monitoring of industrial production chains even in developing countries, which are currently the greatest producers of cosmetics and the major exporters of raw materials. This review aims to highlight the immunological techniques proposed for cosmetics analysis, focusing on the detection of prohibited/regulated compounds, bacteria and toxins, and allergenic substances, and the identification of counterfeits.

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Enzyme-Linked Immunosorbent Assay (ELISA) Technique for Food Analysis

Cited by 6 publications

References 104 publications

A practical guide to in situ and ex situ characterisation of arabinogalactan proteins (AGPs) in fruits

A practical guide to in situ and ex situ characterisation of arabinogalactan proteins (AGPs) in fruits

Possibility of using fatty acid profiles for the authentication of beef adulterated with pork, donkey, and dog meat

Immunological Analytical Techniques for Cosmetics Quality Control and Process Monitoring

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