Enzyme-linked immunosorbent assay for antibodies to native DNA in sera of patients with SLE

Kávai, M; Bányai, A; Zsindely, A.; Sonkoly, I; Szegedi, Gyula

doi:10.1016/0022-1759(82)90191-0

Cited by 33 publications

(7 citation statements)

References 14 publications

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“…Testing was performed by enzyme-linked immunosorbent assay (ELISA) with slight modifications as previously described (27)(28)(29). Heat-denatured calf thymus DNA (Sigma, St. Louis, MO) was dissolved in phosphate buffered saline (PBS) containing 5% calf serum (CS), at a concentration of 1 &ml, and then diluted to obtain optical absorbance of 0.2A at 260 nm.…”

Section: Methodsmentioning

confidence: 99%

Studies of anti‐f(ab')₂ antibodies and possible immunologic control mechanisms in systemic lupus erythematosus

Silvestris

Bankhurst

Searles

et al. 1984

Arthritis & Rheumatism

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Studies of anti‐DNA and anti‐F(ab')2 antibodies were performed by enzyme‐linked immunosorbent assay in 51 patients with systemic lupus erythematosus (SLE). Patients with severe, uncontrolled disease showed high levels of anti‐DNA and low levels of anti‐F(ab')2 antibodies. Patients with quiescent SLE usually showed high levels of anti‐F(ab')2 and low levels of anti‐DNA antibodies. Isolated anti‐F(ab')2 antibodies from autologous SLE remission serum or from the sera of unaffected siblings of SLE patients showed maximum inhibition in test systems using affinity‐purified SLE anti‐DNA antibodies reacting with single‐stranded DNA.

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Section: Methodsmentioning

confidence: 99%

Studies of anti‐f(ab')₂ antibodies and possible immunologic control mechanisms in systemic lupus erythematosus

Silvestris

Bankhurst

Searles

et al. 1984

Arthritis & Rheumatism

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“…Subsequently, antibodies to nuclear components, among which, autoantibodies to DNA have been discovered. Subsequently, research from the 1960s mainly focused on the identification of antibodies against DNA [2,3]. In parallel, Lachman identified that the antigen, playing a pivotal part in the LE phenomenon is not only DNA, but composed of histone proteins, as well as the complex chromatin itself [4].…”

Section: Introductionmentioning

confidence: 99%

Anti-nuscleosome antibody, a reliable indicator for lupus nephritis

Kiss¹,

Lakos²,

Szegedi³

et al. 2009

Autoimmunity

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Systemic lupus erythematosus (SLE) is characterized by the presence of antinuclear antibodies (ANA). ANAs are aimed against native DNA, as well as histone proteins and small nuclear ribonucleoproteins, also could be targeted against the entire chromatin, consisted of nucleosomes. Herein, we assessed the frequency and the concentration of anti-nucleosome antibodies (aNS) in a group of 107 Hungarian patients with SLE. Moreover, correlations between these autoantibody concentrations and disease activity, as well as organ manifestations, mainly renal symptoms were evaluated. The occurrence of positive aNS, anti-dsDNA and anti-histone (aH) antibodies were 39.2, 28.0 and 47.6%, respectively. All the three autoantibodies were represented with significantly higher titers in patients with lupus nephritis. A positive correlation was found between the concentration of these autoantibodies and disease activity. The long disease duration (mean 8.5 years) and consequent low disease activity (mean disease activity index: 3.28) could be the reason for the relatively low occurrence of ANAs amongst patients. Besides anti-dsDNA, the assessment of aNS and aH antibodies could be useful in the diagnosis and follow-up of SLE. The authors discuss the possible role of aNS autoantibodies in the pathogenesis of lupus nephritis.

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“…Sera were collected and kept at − 70°C until analysis. Anti‐dsDNA antibodies were assessed by an ELISA method described previously [ 19]. Levels of C3 and C4 complement components were quantified by single radial immunodiffusion.…”

Section: Methodsmentioning

confidence: 99%

Effect of plasmapheresis on ligand binding capacity and expression of erythrocyte complement receptor type 1 (CR1) of patients with systemic lupus erythematosus (SLE)

Csípő

Kiss

Soltész

et al. 1999

Clinical and Experimental Immunology

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The functional activity and the expression of CR1 on the erythrocytes (E) of patients with SLE were, respectively, determined by measuring the binding to E of either complement-opsonized bovine serum albumin (BSA)-anti-BSA immune complexes (ICC) or specific anti-ECR1 MoAbs. We found that both the functional activity and levels of ECR1 in SLE patients homozygous for ECR1 high density allele were significantly lowered compared with healthy controls having the same allele. Soon after plasmapheresis there was a significant increase in E ICC binding activity, and this increased functional activity was stable. Moreover, plasmapheresis reduced the level of immune complexes demonstrable in the circulation of the patients. The expression of ECR1 determined with several different anti-CR1 MoAbs was also elevated as a consequence of plasmapheresis. This elevation was observed for both MoAb 1B4, which competes for the ICC binding site of ECR1, and for MoAb HB8592, which does not, but the time course for the increase in binding of the two MoAbs was different, in that the epitope recognized by MoAb 1B4 increased more rapidly. The present results, considered in the context of previous findings, suggest that more than one mechanism may be operative with respect to the effects of the plasmapheresis in increasing ECR1 levels defined by different epitopes on the molecule.

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Enzyme-linked immunosorbent assay for antibodies to native DNA in sera of patients with SLE

Cited by 33 publications

References 14 publications

Studies of anti‐f(ab')₂ antibodies and possible immunologic control mechanisms in systemic lupus erythematosus

Studies of anti‐f(ab')₂ antibodies and possible immunologic control mechanisms in systemic lupus erythematosus

Anti-nuscleosome antibody, a reliable indicator for lupus nephritis

Effect of plasmapheresis on ligand binding capacity and expression of erythrocyte complement receptor type 1 (CR1) of patients with systemic lupus erythematosus (SLE)

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Enzyme-linked immunosorbent assay for antibodies to native DNA in sera of patients with SLE

Cited by 33 publications

References 14 publications

Studies of anti‐f(ab')2 antibodies and possible immunologic control mechanisms in systemic lupus erythematosus

Studies of anti‐f(ab')2 antibodies and possible immunologic control mechanisms in systemic lupus erythematosus

Anti-nuscleosome antibody, a reliable indicator for lupus nephritis

Effect of plasmapheresis on ligand binding capacity and expression of erythrocyte complement receptor type 1 (CR1) of patients with systemic lupus erythematosus (SLE)

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Studies of anti‐f(ab')₂ antibodies and possible immunologic control mechanisms in systemic lupus erythematosus

Studies of anti‐f(ab')₂ antibodies and possible immunologic control mechanisms in systemic lupus erythematosus