Enzyme-Linked Immunosorbent Assay for Apolipoprotein C-I

Riesen, Walter; Sturzenegger, E

doi:10.1515/cclm.1986.24.10.723

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…In hyperlipidemic individuals, several groups reported increased plasma levels of apoC1, especially in the context of hypertriglyceridemia or mixed hyperlipidemia, with values reaching 200 mg/L in some studies [ 3 , 43 , 45 ]. However, this phenotype was not always observed [ 13 ].…”

Section: Plasma Levels and Distribution Of Apoc1 Between Lipoproteinsmentioning

confidence: 99%

“…The serum concentration of apoC1 is about 60 mg/L in normolipidemic subjects [3,13,[42][43][44]. ApoC1 is mainly measured by enzyme-linked immunosorbent assay (ELISA) [13,45,46] but the use of mass spectrometry is becoming more and more widespread [47]. ApoC1 is mainly associated with HDL and VLDL in humans [4,48,49].…”

Section: Plasma Levels and Distribution Of Apoc1 Between Lipoproteinsmentioning

confidence: 99%

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Role of apolipoprotein C1 in lipoprotein metabolism, atherosclerosis and diabetes: a systematic review

Rouland

Masson

Lagrost

et al. 2022

Cardiovasc Diabetol

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Apolipoprotein C1 (apoC1) is a small size apolipoprotein whose exact role is not totally clarified but which seems to modulate significantly the metabolism of lipoproteins. ApoC1 is involved in the metabolism of triglyceride-rich lipoproteins by inhibiting the binding of very low density lipoproteins (VLDL) to VLDL-receptor (VLDL-R), to low density lipoprotein receptor (LDL-R) and to LDL receptor related protein (LRP), by reducing the activity of lipoprotein lipase (LPL) and by stimulating VLDL production, all these effects leading to increase plasma triglycerides. ApoC1 takes also part in the metabolism of high density lipoproteins (HDL) by inhibiting Cholesterol Ester Transfer Protein (CETP). The functionality of apoC1 on CETP activity is impaired in diabetes that might account, at least in part, for the increased plasma CETP activity observed in patients with diabetes. Its different effects on lipoprotein metabolism with a possible role in the modulation of inflammation makes the net impact of apoC1 on cardiometabolic risk difficult to figure out and apoC1 might be considered as pro-atherogenic or anti-atherogenic depending on the overall metabolic context. Making the link between total plasma apoC1 levels and the risk of cardio-metabolic diseases is difficult due to the high exchangeability of this small protein whose biological effects might depend essentially on its association with VLDL or HDL. The role of apoC1 in humans is not entirely elucidated and further studies are needed to determine its precise role in lipid metabolism and its possible pleiotropic effects on inflammation and vascular wall biology. In this review, we will present data on apoC1 structure and distribution among lipoproteins, on the effects of apoC1 on VLDL metabolism and HDL metabolism and we will discuss the possible links between apoC1, atherosclerosis and diabetes.

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Section: Plasma Levels and Distribution Of Apoc1 Between Lipoproteinsmentioning

confidence: 99%

Section: Plasma Levels and Distribution Of Apoc1 Between Lipoproteinsmentioning

confidence: 99%

Role of apolipoprotein C1 in lipoprotein metabolism, atherosclerosis and diabetes: a systematic review

Rouland

Masson

Lagrost

et al. 2022

Cardiovasc Diabetol

View full text Add to dashboard Cite

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“…Die Apoproteine C-l und C-lll wurden mit Hilfe eines nicht kompetitiven Sandwich-Elisa unter Verwendung von Kaninchen-bzw. Schaf-Antikörpern gemessen (4). Apo-C-II wurde semiquantitativ durch Elektro-Immundiffusion ermittelt (5), die anti-Apo-C-ll Antikörper stammten vom Kaninchen.…”

Section: Introductionunclassified

Zur klinischen Bedeutung der Apolipoproteine

Riesen¹,

Mordasini²,

Keller³

1985

LaboratoriumsMedizin

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Serum lipids are transported by specific proteins, the apolipoproteins. These apolipoproteins serve various functions, e.g. the activation of enzymes which are important for lipoprotein metabolism or the binding to receptors on celJ membranes. Apolipoproteins, therefore, are not only ofinterest for diagnostic purpose, but also to get insight into the pathogenetic mechanisms, which lead to lipid disorders. The diagnostic relevance is illustrated with studies on two groups ofpatients with pectanginous complaints: one group consisting of patients with significant stenoses of the coronary arteries, and one group without stenoses. The combined determination ofapo-l and cholesterol proved to be the best discriminant between the two groups. The pathogenetic relevance of apolipoprotein measurement is demonstrated with 6 patients suffering from chylomicronemia. Four out of these displayed an apo C-ll deficiency which was the cause of the absence of lipoprotein lipase activity, one patient showed a deficiency of the enzyme itself, while one patiem had a chylomicronemia of unknown origin, since both apo C-ll and lipoprotein lipase activity were within normal values.-

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Cyclodextrin‐micellar electrokinetic chromatography of apolipoproteins on human very low‐density lipoprotein

et al. 2020

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The apolipoproteins (APOs) of human very low‐density lipoprotein (VLDL) were investigated by an optimized cyclodextrin‐micellar electrokinetic chromatography (CD‐MEKC) method. The separation buffer consisted of 20 mM sodium phosphate, 40 mM bile salts (50% sodium cholate and 50% sodium deoxycholate), 25 mM carboxymethyl‐β‐cyclodextrin (CM‐β‐CD) (pH 7.0). For CD‐MEKC separation, a sample injection time of 12 s, a separation voltage of 15 KV, and a capillary temperature of 15°C were chosen. The optimal CD‐MEKC method showed good resolution and repeatability for VLDL APOs. Identification and quantitation of VLDL APOs CI, CIII, and E were based on comparison with human APO standards. Good linear relationships with correlation coefficient (R2) 0.99 were obtained for APOs CI, CIII, and E standards. For these three APOs, the linear ranges were within 0.01‐0.54 mg/mL, and the concentration limits of detection (LODs) were lower than 0.02 mg/mL. Moreover, VLDL APOs from four uremic patients and four healthy subjects were compared. The uremic and healthy CD‐MEKC profiles showed dramatic difference. The levels of APO CIII were significantly higher for two patients, and the level of APO E was significantly higher for one patient. This study might be helpful for following the disease development of uremia and cardiovascular disease (CVD) in the future.

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Enzyme-Linked Immunosorbent Assay for Apolipoprotein C-I

Cited by 5 publications

References 21 publications

Role of apolipoprotein C1 in lipoprotein metabolism, atherosclerosis and diabetes: a systematic review

Role of apolipoprotein C1 in lipoprotein metabolism, atherosclerosis and diabetes: a systematic review

Zur klinischen Bedeutung der Apolipoproteine

Cyclodextrin‐micellar electrokinetic chromatography of apolipoproteins on human very low‐density lipoprotein

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