Enzyme-linked immunosorbent assay for detecting antibodies in cattle in a herd in which anaplasmosis was diagnosed

Thoen, Charles O.; Blackburn, Billie O.; Mills, Ken; Lomme, Jon; Hopkins, Matthew

doi:10.1128/jcm.11.5.499-502.1980

Cited by 17 publications

(4 citation statements)

References 6 publications

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“…Indirect immunoperoxidase staining was applied to detect BAdV type III and BEV antibodies, as described by RUIZ et al (1988) andLAGER et al (1981). Protein A was used to detect llama antibodies (THOEN, 1980 (ESTES, 1980;SAIF, 1988). Agar-gel immunodiffusion tests (AGID) were used to detect group-specific BTV antibodies (PEAR- SON and JOCHIM, 1979), BLV (MILLER, 1980), and FMDV (MCVICAR, 1970).…”

Section: Laboratory Testingmentioning

confidence: 99%

Serological Survey of Viral Antibodies in Llamas (Lama glama) in Argentina

Puntel

Fondevila

Viera

et al. 1999

Journal of Veterinary Medicine, Series B

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This study analysed sera from 390 llamas (Lama glama) from nine farms located in three different Argentine provinces: Buenos Aires, Cordoba and Jujuy. The samples were tested for antibodies against 8 virus known to infect cattle: bovine herpesvirus type 1 (BHV-1), bovine viral diarrhea virus (BVDV), bovine adenovirus (BAdV III), bovine enterovirus (BEV), bovine rotavirus (BRV), bluetongue virus (BTV), bovine leukaemia virus (BLV), and foot-and-mouth virus (FMDV) by conventional methods such as seroneutralization, immunoperoxidase staining, and agar gel immunodiffusion. The antibody prevalences detected in llamas were: BHV-1 in 0.77% (3/390), BVDV in 2.05% (8/390), BAdV III in 5.13% (20/390), BEV in 4.10% (16/390), BRV in 87.69% (342/390). No antibodies against BTV, BLV and VIAA (FMDV infection associated antigen) were detected.

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Section: Laboratory Testingmentioning

confidence: 99%

Serological Survey of Viral Antibodies in Llamas (Lama glama) in Argentina

Puntel

Fondevila

Viera

et al. 1999

Journal of Veterinary Medicine, Series B

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“…(4,10). The specificity of the current tests, including complement fixation (24,31), card agglutination (2), capillary tube agglutination (40), enzyme-linked immunosorbent assay (4, 10,45,46), indirect immunofluorescence (14)(15)(16)30), immunoblots (1), and radioimmunoassay (42), could be improved by using purified A. marginale antigen. Another part of the error is false-negative reactions occurring with some tests (26) either because of low sensitivity or because some nonprimary binding tests, such as complement fixation, do not detect all immunoglobulin isotypes (27).…”

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confidence: 99%

Identification of Anaplasma marginale long-term carrier cattle by detection of serum antibody to isolated MSP-3

et al. 1991

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Rapid and accurate detection of Anaplasma marginale-infected cattle would enhance anaplasmosis control procedures and evaluation of vaccines. Current tests based on detection of antibodies in serum are not widely used for several reasons, including the occurrence of either false-positive or false-negative results. We evaluated binding of antibodies in serum to a subunit antigen isolated from A. marginale initial bodies-major surface protein 3 (MSP-3). MSP-3 was detected in lysates of eight geographically different isolates of A. marginale and purified by affinity chromatography with monoclonal antibody AmG75C2. Antibodies from cattle infected with any of five geographically different isolates of A. marginale reacted in immunoblots with MSP-3. Sera from uninfected cattle and cattle infected with another rickettsial organism and two hemoprotozoal organisms failed to react with MSP-3. Six carrier cattle infected with the Florida isolate of A. marginale had antibody titers to MSP-3 ranging from i03 to 106 during a 5-year evaluation period. Since specific antibodies to isolated MSP-3 persist in high titers in long-term carrier cattle sera and MSP-3 is common among A. marginale isolates, it is recommended as a subunit antigen for an anaplasmosis test.

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“…Properties of the A. marginale ELISA with particulate antigen were investigated earlier (18). Advantages of ELISA over CFT were pointed out.…”

Section: T3-mentioning

confidence: 99%

“…Because it is less complex than CFT and can be fully automated, it is suitable for the screening of large numbers of serum samples. Lack of specificity was attributed to anti-species conjugates (18). Subsequently, improved results were obtained with protein A-HRPO conjugates.…”

Section: T3-mentioning

confidence: 99%

Detection of antibodies to Anaplasma marginale by an improved enzyme-linked immunosorbent assay with sodium dodecyl sulfate-disrupted antigen

Winkler¹,

Brown²,

Lutz³

1987

J Clin Microbiol

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Sensitivities of two enzyme-linked immunosorbent assays (ELISAs) with particulate and sodium dodecyl sulfate (SDS)-disrupted Anaplasma marginale antigen were compared. The quotient of positive reference sera divided by the absorbance quotient of a negative reference serum at identical dilution was termed the signal-to-noise ratio. Optimal signal-to-noise ratios were dependent on both pretreatment of antigen and antigen concentration. SDS disruption of anaplasmal antigen resulted in a markedly improved signal-to-noise ratio of ELISA compared with ELISA with untreated antigen at identical antigen and serum dilutions. This represented higher sensitivity and lower background absorbance of the ELISA with disrupted antigen. SDS-disrupted A. marginale antigen was standardized by protein determination, and antigen, as well as precoated microtiter wells, was stored frozen without apparent loss of antigenic properties. ELISA results were in agreement with results of positive and negative control sera tested by the complement fixation test or by light microscopy Anaplasma diagnosis in Giemsa-stained blood films. Different intraerythrocytic rickettsia of the species Anaplasma are the cause of hemotropic disease in wild and domestic ruminants (6). Anaplasma marginale is the principal pathogenic agent in cattle. Worldwide distribution and significance in cattle production areas have been reported (8, 15). The disease is transmitted by a variety of biting flies and numerous ixodid tick species (5, 8, 14). Transmissibility by ixodid ticks may be isolate restricted (17). Transplacental and iatrogenic transmission have been reported (13, 19). Recently, attempts were made to develop an isolatecommon surface protein subunit vaccine for cattle which appears promising (11). Common diagnostic techniques for A. marginale include light microscopy of Giemsa-stained blood smears, card agglutination, and the complement fixation test (CFT). The indirect fluorescent-antibody test (microfluorometry) (3, 10), passive hemagglutination, capillary tube agglutination, the latex agglutination test (9), and the enzyme-linked immunosorbent assay (ELISA) (1, 12, 18) are other diagnostic assays. The ELISA is a simple, rapid, and sensitive assay that is appropriate for screening greater numbers of serum samples (18). The present study was done to develop an ELISA with improved sensitivity and standardized solid face coating for Anaplasma serology. Optimal antigen pretreatment and antigen dilution for solid face coating were determined on the basis of absorbance of the final substrate reaction product. Absorbance of positive over negative reference sera at similar conjugate dilution was used as a descriptor of signalto-noise ratio for comparison of optimal antigen concentration and immunosorbent characteristics.

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Enzyme-linked immunosorbent assay for detecting antibodies in cattle in a herd in which anaplasmosis was diagnosed

Cited by 17 publications

References 6 publications

Serological Survey of Viral Antibodies in Llamas (Lama glama) in Argentina

Serological Survey of Viral Antibodies in Llamas (Lama glama) in Argentina

Identification of Anaplasma marginale long-term carrier cattle by detection of serum antibody to isolated MSP-3

Detection of antibodies to Anaplasma marginale by an improved enzyme-linked immunosorbent assay with sodium dodecyl sulfate-disrupted antigen

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