Enzyme-linked Immunosorbent Assay for Pierce's Disease Bacteria in Plant Tissues

Nomé, S. F.; Raju, B. C.; Goheen, A. C.; Nyland, G.; Docampo, Delia M.

doi:10.1094/phyto-70-746

Cited by 37 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Immunoglobulins were purified by precipitation with 50% saturated ammonium sulfate and by dialysis (35). Dilutions of rabbit antibody for ELISA reactions were done as described previously (29). Ascitic fluid immunoglobulins were diluted 1:1,200, 1:2,400, and 1:4,800.…”

Section: Methodsmentioning

confidence: 99%

Xylella fastidiosa gen. nov., sp. nov: Gram-Negative, Xylem-Limited, Fastidious Plant Bacteria Related to Xanthomonas spp.

Wells

Raju

Hung

et al. 1987

International Journal of Systematic Bacteriology

539

308

View full text Add to dashboard Cite

Twenty-five phenotypically and genotypically similar strains of a fastidious, xylem-limited bacterium were isolated from 10 plant disease sources including Pierce's disease of grapevines, phony disease of peach, periwinkle wilt, and leaf scorches of almond, plum, elm, sycamore, oak, and mulberry. The cells were single (occasionally filamentous), nonmotile, aflagellate straight rods (0.25 to 0.35 by 0.9 to 3.5 pm). They were gram negative, catalase positive, and oxidase negative, utilized hippurate, and produced gelatinase and often beta-lactamase but not beta-galactosidase, coagulase, lipase, amylase, phosphatase, indole, or HZS. The bacteria were strict aerobes with optimum growth at 26 to 28°C and pH 6.5 to 6.9 and had doubling times of 0.45 to 1.98 days in periwinkle wilt broth. Monoclonal antibodies prepared against the Pierce's disease bacterium reacted with all strains. DNA composition was 51 to 53 mol% guanine plus cytosine, and strains were at least 85% related in DNA hybridization. Sequencing of 16s ribosomal ribonucleic acid related them to the xanthomonads. These bacteria form a distinct group, and the name XyZeZZa fastidiosa is proposed, establishing a new genus with one species in the gamma subgroup of the eubacteria. Strain PCE-RR (ATCC 35879) is designated as the type strain. (13,16,26,27,33,36,37,41,44,45; J. Sherald, unpublished data).

show abstract

Section: Methodsmentioning

confidence: 99%

Xylella fastidiosa gen. nov., sp. nov: Gram-Negative, Xylem-Limited, Fastidious Plant Bacteria Related to Xanthomonas spp.

Wells

Raju

Hung

et al. 1987

International Journal of Systematic Bacteriology

539

308

View full text Add to dashboard Cite

show abstract

“…XLB, also referred to as rickettsia-like bacteria, have been isolated from diseased plants, insect vectors, and apparently healthy reservoir plants (8,15,26), indicating a complexity of XLB-host-vector interactions. Studies on etiology (14, 15, 191, culture requirements (6, 8, 25), serology (7,12,20,22,26), and certain biochemical characteristics (15, 24) have indicated that the XLB are not related to the major phytopathogen-containing genera of gram-negative bacteria and can be subdivided into at least two closely related subgroups of unknown taxonomic distinction or affiliation.In this study, the relatedness of several XLB to each other and to other gram-negative phytopathogens was examined and defined by the following three criteria: deoxyribonucleic acid (DNA) base composition, genome molecular weight, and DNA homology.(The results were taken from a thesis presented by S.M.K. to Florida State University, Tallahassee.…”

mentioning

confidence: 99%

Genetic Relationships of Some Fastidious Xylem-Limited Bacteria

Kamper

French

DeKloet

1985

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

Fastidious, gram-negative xylem-limited bacteria were examined for genetic relatedness to each other and to members of the major phytopathogen-containing genera of gram-negative bacteria. All of the xylem-limited bacterial strains studied had deoxyribonucleic acid base compositions of approximately 50.5 mol% guanine plus cytosine and genome molecular weights of 1.4 X lo9 2 0.2 X lo9. The results of our deoxyribonucleic acid-deoxyribonucleic acid hybridization experiments suggested that these strains are all related at the species level, but supported distinction at the varietal level, and possibly at the subspecies level between the bacteria causing Pierce's disease of grapevine and the bacteria causing phony disease of peach, plum leaf scald, and periwinkle wilt. No relatedness was detected between the xylem-limited bacteria and Erwinia chrysanthemi, Pseudomonas syringae pv. syringae, Xanthomonas campestris pv. campestris, or Agrobacterium tumefaciens.The significance of the recently discovered fastidious gram-negative xylem-limited bacteria (XLB) in plant diseases has become increasingly evident (8,15). XLB, also referred to as rickettsia-like bacteria, have been isolated from diseased plants, insect vectors, and apparently healthy reservoir plants (8,15,26), indicating a complexity of XLB-host-vector interactions. Studies on etiology (14, 15, 191, culture requirements (6, 8, 25), serology (7,12,20,22,26), and certain biochemical characteristics (15, 24) have indicated that the XLB are not related to the major phytopathogen-containing genera of gram-negative bacteria and can be subdivided into at least two closely related subgroups of unknown taxonomic distinction or affiliation.In this study, the relatedness of several XLB to each other and to other gram-negative phytopathogens was examined and defined by the following three criteria: deoxyribonucleic acid (DNA) base composition, genome molecular weight, and DNA homology.(The results were taken from a thesis presented by S.M.K. to Florida State University, Tallahassee.) MATERIALS AND METHODSBacterial strains and culture conditions. Two strains of the plum leaf scald bacterium, strains PLSB-40 and PLSB-53, and one strain of the phony peach disease bacterium, strain PPDB-2.1, were isolated by W. J. French at Monticello, Fla. (strains PLSB-40 and PPDB-2.1) or Cascata, Rio Grande do Sul, Brazil (strain PLSB-53). One strain of the Pierce's disease bacterium, strain PDB-1, was provided by N. W. Schaad, University of Idaho, Moscow, and one strain of periwinkle wilt bacterium, strain PWB-1, was provided by M. J. Davis, University of Florida, Ft. Lauderdale. Stationary cultures of strains PLSB-40, PLSB-53, PPDB-2.1, and PWB-1 in PW broth (8) and shaker cultures of strain PDB-1 in PD2 broth (10) were grown at 28°C for 5 to 7 days. Erwinia chrysanthemi A-17, Xanthomonas campestris B-24, Pseudomonas syringae C-7, and Agrobacterium tumefaciens 84G-102 were provided by N. W. Schaad, Escherichia coli K-12 C600 was provided by H. J. Taylor, Florida State * Corresponding aut...

show abstract

“…Prior to the classification of X. fastidiosa subspecies, X. fastidiosa was identified by a variety of techniques including: microscopy, culturing, enzyme-linked immunosorbent assay, standard polymerase chain reaction (PCR), RAPD fingerprinting and western blotting [18][19][20][21][22][23][24][25]. Both microscopy and culturing are slow and relatively inaccurate methods for identification of X. fastidiosa and do not allow for identification of specific subspecies [12].…”

Section: Introductionmentioning

confidence: 99%

Development of Quantitative Real-Time Polymerase Chain Reaction protocols for rapid detection and differentiation of Xylella fastidiosa subsp. fastidiosa and Xylella fastidiosa subsp. multiplex

Pierce¹,

Morano²,

Bextine³

2011

J Plant Pathol Microbiol

View full text Add to dashboard Cite

Enzyme-linked Immunosorbent Assay for Pierce's Disease Bacteria in Plant Tissues

Cited by 37 publications

References 0 publications

Xylella fastidiosa gen. nov., sp. nov: Gram-Negative, Xylem-Limited, Fastidious Plant Bacteria Related to Xanthomonas spp.

Xylella fastidiosa gen. nov., sp. nov: Gram-Negative, Xylem-Limited, Fastidious Plant Bacteria Related to Xanthomonas spp.

Genetic Relationships of Some Fastidious Xylem-Limited Bacteria

Development of Quantitative Real-Time Polymerase Chain Reaction protocols for rapid detection and differentiation of Xylella fastidiosa subsp. fastidiosa and Xylella fastidiosa subsp. multiplex

Contact Info

Product

Resources

About