Enzyme-linked immunosorbent assay for the detection of serum antibody to outer membrane proteins of Treponema pallidum.

Morrison-Plummer, J; Alderete, John F.; Baseman, Joel B.

doi:10.1136/sti.59.2.75

Cited by 9 publications

(6 citation statements)

References 16 publications

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“…challenge, have high titers of CIC which develop after 3-7 weeks (12,13). As iti liutiian syphilis, these CIC in rabbits have been sliowti to cotitain specific treponemal antigens which are beitig further characterized (14,15).…”

mentioning

confidence: 99%

Rabbit model of disseminated syphilis: immunoblot and immunohistologic evidence for a role of specific immune complexes in lesion pathogenesis

et al. 1988

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Circulating immune complexes (CIC) containing Treponema pallidum proteins have been preliminarily implicated as inducers of a neutrophilic vascular reaction in early human cutaneous lesions of secondary syphilis. To clarify the role of specific CIC in producing cutaneous and renal lesions, 12 rabbits were studied at the following intervals after induction of disseminated syphilis: 20 days (4 rabbits: biopsies of normal and lesional skin for direct immunofluorescence (IMF) for (IgG, IgM, IgA, Clq, C3, C4), fibrin, and T. pallidum proteins; routine histology; and immunoblots of serum for CIC containing T. pallidum proteins); 21 days (4 rabbits: as at 20 days without IMF for T. pallidum protein); 23 days (4 rabbits: as at 20 days without IMF); 30 days (same 12 rabbits restudied with routine histology of normal and lesional skin; kidneys from 4 rabbits removed for routine, IMF, and electron microscopy (EM). Treponemal polypeptide antigen (MW-87 kd) was demonstrated in CIC from rabbits. Routine cutaneous histology showed evolution of lesions from an early neutrophilic vascular reaction to the typical lymphoplasmacytic reaction. IMF showed vessel-based immunoreactants in 3 of the 4 rabbits tested at 20 days and 1 of 4 at 21 days, and T. pallidum proteins in 3 of 4 rabbits at 20 days. Routine histology, IMF, and EM studies of glomeruli showed glomerular abnormalities, but no evidence of immune deposits containing specific T. pallidum protein. Skin and kidney studies of 4 controls were all negative. These data indicate a role for specific immune complexes in the pathogenesis of cutaneous lesions in this rabbit model.

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confidence: 99%

Rabbit model of disseminated syphilis: immunoblot and immunohistologic evidence for a role of specific immune complexes in lesion pathogenesis

et al. 1988

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“…The effects of these two conditions, fixation and drying, were more dramatic in our system. This result could possibly have been caused by the difference in the microtiter plates; Morrison-Plummer et al (6) used polyvinylchloride, whereas we used polystyrene.…”

Section: Methodsmentioning

confidence: 90%

“…The pH of the adsorption buffer did not appear to be too critical with either fixed or nonfixed sonic extracts. Morrison-Plummer et al (6) also observed a reduction in the reactivity of rabbit sera with ethanol-fixed whole T. pallidum cells as compared with purified outer membrane proteins. The effects of these two conditions, fixation and drying, were more dramatic in our system.…”

Section: Methodsmentioning

confidence: 93%

Enumeration of Treponema pallidum cells cultivated in vitro by an enzyme-linked immunosorbent assay

Cox¹,

Moeckli²,

Keaney³

1984

Infect Immun

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An enzyme-linked immunosorbent assay was developed to enumerate Treponema pallidum cells. The assay could detect from 2 x 1 to 4 x 10 treponemes per ml. Reactive rabbit serum and goat anti-rabbit immunoglobulin G (peroxidase conjugate) were used in the assay. Optimum results were obtained when 2,2'-azino-di(ethylbenzthiazolinesulfonic acid) was used as the dye for the enzyme reaction and the reactions were allowed to run for 45 min. Interestingly, assays in which in vivo-cultivated T. pallidum was used produced lower absorbance values than those in which T. pallidum was cultivated in vitro.

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“…Surface properties are fundamental in determining the interaction between bacteria and the host's immune and non-specific defences, and several groups have attempted to identify important surface components by surface labelling or receptor-ligand binding experiments. Baseman and colleagues [3,14,[41][42][43][44][45] have made extensive use of these procedures, but comparison of their work with that of others is often difficult, due to heavy exposure of autoradiographs of gel profiles, which obscures the pattern of prominent polypeptides we have described. Also, use of lower concentrations of acrylamide in gels than those used by most groups, and often of M r markers of undefined origin, makes comparison difficult.…”

Section: Studies Of the Treponemal Surfacementioning

confidence: 99%

Molecular and immunochemical analysis ofTreponema pallidum

Penn

Bailey

1986

FEMS Microbiology Letters

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Molecular analysis of polypeptides and antigens of Treponema pallidum has been used increasingly during the past 5 years in investigation of the immunology, pathogenicity and molecular biology of this organism. Failure to culture the organism has severely limited our knowledge of its constituent polypeptides and antigens, but many profiles of these unknown constituents, revealed by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and immunoblotting techniques have been published. In order to compare meaningfully the results obtained by different groups, we have identified a standard pattern of prominent ‘landmark’ polypeptides in such gel profiles and where possible have assigned functional identities to them. A preliminary nomenclature for the prominent polypeptides of T. pallidum is proposed. These are: P1, 80 kDa; P2, 60 kDa; P3, 47 kDa, an outer membrane‐associated polypeptide; P4, 40 kDa; P5, 37 kDa, the major polypeptide of the axial filament; P6, 34 kDa; and P7, 31.5 kDa.

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Enzyme-linked immunosorbent assay for the detection of serum antibody to outer membrane proteins of Treponema pallidum.

Cited by 9 publications

References 16 publications

Rabbit model of disseminated syphilis: immunoblot and immunohistologic evidence for a role of specific immune complexes in lesion pathogenesis

Rabbit model of disseminated syphilis: immunoblot and immunohistologic evidence for a role of specific immune complexes in lesion pathogenesis

Enumeration of Treponema pallidum cells cultivated in vitro by an enzyme-linked immunosorbent assay

Molecular and immunochemical analysis ofTreponema pallidum

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