Enzyme‐Linked immunosorbent assay for the detection and identification of coxsackie b antigen in tissue cultures and clinical specimens

Yolken, Robert H.; Torsch, V M

doi:10.1002/jmv.1890060107

Cited by 34 publications

(16 citation statements)

References 13 publications

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“…By using an enzyme-linked immuno-5 VOL. 2,1989 sorbent assay (ELISA), virus was detected in 8 of 11 stools from which coxsackievirus was grown (165 (59,61,94). A rapid report of a presumptive diagnosis of enterovirus infection based on CPE in cell culture, without waiting for the specific typing of the isolate, can have an impact on patient management (28,157).…”

Section: Rabiesmentioning

confidence: 99%

Role of the virology laboratory in diagnosis and management of patients with central nervous system disease

1989

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A number of viruses cause acute central nervous system disease. The two major clinical presentations are aseptic meningitis and the less common meningoencephalitis. Clinical virology laboratories are now more widely available than a decade ago; they can be operated on a modest scale and can be tailored to the needs of the patients they serve. Most laboratories can provide diagnostic information on diseases caused by enteroviruses, herpesviruses, and human immunodeficiency virus. Antiviral therapy for herpes simplex virus is now available. By providing a rapid diagnostic test or isolation of the virus or both, the virology laboratory plays a direct role in guiding antiviral therapy for patients with herpes simplex encephalitis. Although there is no specific drug available for enteroviruses, attention needs to be paid to these viruses since they are the most common cause of nonbacterial meningitis and the most common pathogens causing hospitalization for suspected sepsis in young infants in the United States during the warm months of the year. When the virology laboratory maximizes the speed of viral detection or isolation, it can make a significant impact on management of these patients. Early viral diagnosis benefits patients with enteroviral meningitis, most of whom are hospitalized and treated for bacterial sepsis or meningitis or both; these patients have the advantage of early withdrawal of antibiotics and intravenous therapy, early hospital discharge, and avoidance of the risks and costs of unnecessary tests and treatment. Enteroviral infection in young infants also is a risk factor for possible long-term sequelae. For compromised patients, the diagnostic information helps in selecting specific immunoglobulin therapy. Good communication between the physician and the laboratory will result in the most benefit to patients with central nervous system viral infection.

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Section: Rabiesmentioning

confidence: 99%

Role of the virology laboratory in diagnosis and management of patients with central nervous system disease

1989

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“…Many enteroviruses do not grow in cell lines commonly used. Direct detection of enterovirus antigen or nucleic acid in clinical approach still suffers from inadequate sensitivity [Herrmann et al, 1979;Yolken and Torsch, 1981;Hyypia et al, 1984;Rotbart and Levin, 19871. A reliable serological method not involving cultivation of virus would therefore be a very attractive alternative.…”

Section: Introductionmentioning

confidence: 99%

Comparison of five elsa assays for IgG antibody against coxsackievirus B1

Torfason

Galindo

Keyserling

1988

Journal of Medical Virology

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Enterovirus type and group specificities of five different IgG ELISA methods were compared, using neutralization titration tests as an indicator of the presence or absence of antibodies to coxsackie B (CB) viruses. One of the ELISA assays was a "standard" IgG assay, where the solid phase was coated directly with the purified virus, followed by incubations with human serum, biotinylated anti-human-IgG, streptavidin-peroxidase, and the substrate/chromogen. In a modified standard assay, blocking of common epitopes was attempted by incubating the CB1 virus antigen on the solid phase with a rabbit antiserum to CB5 before the human serum was added. In another modification the serum dilution buffer contained heat-denatured heterologous enteroviruses in an attempt to consume human antibodies reacting with common epitopes. In one assay the purified CB1 virus was captured by purified horse anti-CB1 IgG on the solid phase, before incubation with human serum. In the last of the five assays the serum specimen was incubated with CB1 virus (in the liquid phase) before the virus or virus-antibody complex was captured with purified horse anti-CB1-IgG. Reactions against common antigens dominated in the first three assays. The antigen-capture assay appeared to be at least predominantly type specific. Our data indicate that the liquid-phase assay may be type specific, but more studies are needed. The method of virus purification was critical for the type specificity of the antigen-capture and liquid-phase assays.

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“…The broad antigenic diversity among the nearly 70 different serotypes of EVs and over 100 different serotypes of HRVs has made immunoassays impractical (Yolken & Torsch, 1980, 1981Dearden & A1-Nakib, 1987). Nucleic acid hybridization assays have been used but they lack sensitivity (Rotbart et al, 1984;A1-Nakib et al, 1986;Bruce et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Detection and differentiation of picornaviruses in clinical samples following genomic amplification

Olive

Al-Mufti²,

Al-Mulla

et al. 1990

Journal of General Virology

141

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Enzyme‐Linked immunosorbent assay for the detection and identification of coxsackie b antigen in tissue cultures and clinical specimens

Cited by 34 publications

References 13 publications

Role of the virology laboratory in diagnosis and management of patients with central nervous system disease

Role of the virology laboratory in diagnosis and management of patients with central nervous system disease

Comparison of five elsa assays for IgG antibody against coxsackievirus B1

Detection and differentiation of picornaviruses in clinical samples following genomic amplification

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