Enzyme loading in a solid support with nonuniform pore size distribution

Abstract

The concept of pore size distribution is incorporated into the Clark model of enzyme immobilization in the present study. This refined model predicted that in the case of small harmonic pore radius with the same surface area and porosity of the support, more enzyme could be loaded in a support with nonuniform pores than that with uniform pores. In comparing the enzyme loading efficiency of the support with two different pore size distributions, the one with Gaussian distribution had the greater amount of enzyme immobilized than the other one with Rajagopalan's distribution. Furthermore, more enzyme could be loaded in a support with wider Gaussian pore size distribution than that with narrower distribution. The immobilized enzyme profile in the solid support with pore size distribution displayed a stepwise pattern which differed appreciably from the sigmoidal profile predicted for the support with uniform pore size. This stepwise enzyme distribution profile became sigmoidal with decreasing h(T) or increasing k. The new model could be used for designing protocols for an enzyme immobilization process.

Expression of nucleotide excision repair genes and the risk for squamous cell carcinoma of the head and neck

“…This gives reasonable predictions of the diffusion coefficient in a dilute solution as long as no major conformational changes occur as a result of charges, for example. Several other predictive equations can be found in the literature 9, 18, 19…”

“…Loh et al17 studied pore size distributions using mercury intrusion experiments and found similar results for other porous media. Chiang et al18 compared different pore size distributions for enzyme loading. Li et al19 calculated average pore diameters for agarose and alginate gels, giving an average pore diameter in good agreement with the data of Medin.…”

Coupled diffusion and adsorption effects for multiple proteins in agarose gel

“…Loh et al 17 studied pore size distributions using mercury intrusion experiments and found similar results for other porous media. Chiang et al 18 compared different pore size distributions for enzyme loading. Li et al 19 calculated average pore diameters for agarose and alginate gels, giving an average pore diameter in good agreement with the data of Medin.…”