Enzyme localization in proteins separated by paper electrophoresis in human seminal plasma

Povoa, H

doi:10.1007/bf02172170

Cited by 3 publications

(1 citation statement)

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“…This large number of enzymes, especially proteases present in seminal plasma as HBPs, is not surprising as it has been described that almost 60% of the seminal fl uid proteome is involved in enzymatic activity (Povoa 1962;Pilch and Mann 2006). Much evidence has been provided to show that proteases may be active partners in establishing and maintaining testicular architecture, and in facilitating germ cell migration throughout the spermatogenic developmental processes.…”

Section: Discussionmentioning

confidence: 93%

Proteomic analysis of heparin-binding proteins from human seminal plasma: a step towards identification of molecular markers of male fertility

et al. 2009

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Glycosaminoglycans, especially heparin, are involved in various cell processes such as apoptosis, cell cycle control, platelet activation, capacitation, acrosome reaction and sperm decondensation. Heparin-binding proteins (HBPs) are essential constituents of human seminal fluid, which bind to sperm lipids containing the phosphorylcholine group and mediate the fertilization process. We utilized a proteomic set-up consisting of affinity chromatography, isoelectric focusing (IEF) coupled with matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI TOF/MS) for protein analysis of human HBPs. We resolved 70 different spots on two-dimensional (2-D) gel and subsequently identifi ed these proteins. Forty different types of proteins were identified. Functional analysis revealed that 38% of the proteins belonged to the enzyme category, 20% were involved in RNA processing and transcription, 18% in structure and transport function, and 16% in cell recognition and signal transduction. We also identified 8% of proteins with unknown functions, although their expression in seminal fluid has been documented. Proteins of seminal fluid that bind heparin may be directly involved in sperm capacitation and acrosome reaction (AR), which are the two critical steps for fertilization. This information on HBPs would be useful for identifying potential biomarkers of fertility in the near future.

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Section: Discussionmentioning

confidence: 93%