Enzyme mass transfer coefficient in a sieve plate extraction column

Bhawsar, Prafulla C.M.; Pandit, Aniruddha B.; Sawant, S.B.; Joshi, Jyeshtharaj B.

doi:10.1016/0923-0467(94)87012-8

Cited by 8 publications

(8 citation statements)

References 21 publications

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“…Sieve plate columns are preferred over spray columns for their uniqueness in providing repeated coalescence and redispersion of drops and for reducing backmixing [6] (Fig. 3a).…”

Section: Overall Mass Transfer Coefficientsmentioning

confidence: 99%

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Xylanase mass transfer studies in aqueous two-phase systems using spray and sieve plate columns

Igarashi

Kieckbusch

Franco

2004

Bioprocess and Biosystems Engineering

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Aqueous two-phase systems (ATPSs) have long been used for biomolecule partitioning; these systems offer the possibility of using continuous or semicontinuous extraction processes. They require relatively simple equipment like spray or sieve plate columns that can be adapted for use in ATPSs. The aim of this work was to study the semicontinuous extraction of a model enzyme, xylanase, in spray and sieve plate columns, since, unlike centrifugal contactors, the cost of construction and maintenance of this equipment is low and it is easy to operate. For the spray column, the dispersed phase hold-up and overall mass transfer coefficients K(D) a were evaluated for different column heights and for different superficial velocities of the dispersed phase (light phase). Results indicated that an increase in superficial velocity in the range of 0-0.18 mm/s of the dispersed phase had a positive effect on K(D) a and on hold-up in all column heights studied, 75, 161 and 246 mm. For the same superficial velocity of the dispersed phase, the larger the hold-up was, the shorter the column. For the sieve plate column, the effects of the superficial velocity of the dispersed phase and the number of plates were also studied. Results showed that the K(D) a and hold-up increased with an increase in both parameters. The selectivity of separation of xylanase and BSA (model contaminant) was very high, since 60% of the enzyme was extracted in the light phase, whereas no significant amount of BSA was extracted. The possibility of using the sieve plate column in continuous operation for enzyme extraction was studied because previous work had only addressed the semicontinuous extraction of enzyme. The residence time distribution of the PEG phase using different superficial velocities of the salt phase was studied in continuous operation. The time required to reach the steady state was 40 min, and 70% of the xylanase was recovered. It was found that the Modified Power Spline software was well adjusted to the experimental results.

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“…Sieve plate columns are preferred over spray columns for their uniqueness in providing repeated coalescence and redispersion of drops and for reducing backmixing [6] (Fig. 3a).…”

Section: Overall Mass Transfer Coefficientsmentioning

confidence: 99%

“…Another type of extraction column that has been successfully used in a great variety of extraction processes is the sieve plate column. Sieve plate columns provide repeated coalescence and redispersion of drops and reduce backmixing [5,6].…”

Section: Introductionmentioning

confidence: 99%

Xylanase mass transfer studies in aqueous two-phase systems using spray and sieve plate columns

Igarashi

Kieckbusch

Franco

2004

Bioprocess and Biosystems Engineering

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“…The characteristic velocities, the experimentally determined terminal velocities and the calculated terminal velocities using eqns (6), (8) and (9) are given in Table 5.…”

Section: Conclusion and Discussionmentioning

confidence: 99%

Flooding characteristics of aqueous two-phase systems in a countercurrent sieve-plate column

Hamidi

Berlo

Luyben

et al. 1999

J. Chem. Technol. Biotechnol.

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: The hydraulic capacity of countercurrent liquid-liquid extractors is constrained by its ýooding characteristics. This study presents ýooding data for aqueous two-phase systems containing PEG 4000 and at various concentrations in a countercurrently operated sieve-plate column in Na 2 SO 4 the absence of solutes. Flooding limits, average droplet sizes and dispersed phase hold-ups at ýooding conditions were measured as well as densities, interfacial tensions and viscosities. A correlation proposed by Richardson and Zaki (Trans IChemE 32 :35-53 (1954)) was used successfully for the description of the slip velocities and thus maximum column throughputs.1999 Society of Chemical Industry ( Keywords : aqueous two-phase systems ; bioseparation ; countercurrent extraction ; sieve-plate columnTerminal velocity of a droplet of dispersed phase in the continuous phase (m s~1)

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“…Further, dynamic hold up requires to determine the interfacial area of contact between the dispersed phase droplets and the continuous phase. The dynamic hold-up, the total hold-up and the static hold-up were determined by the procedure suggested by Bhawsar et al, (1994) from the following equations:…”

Section: Hold Up Measurementmentioning

confidence: 99%

“…The interfacial area in a plate column depends on the dispersed phase hold up and drop sizes which further depend on various geometrical and operating parameters. Most of the work reported on the sieve plate column is dedicated to conventional liquid-liquid extraction or aqueous two-phase extraction systems (Igarshi et al, 2004;Hamidi et al, 1999;Bhawsar et al, 1994;Muthuravichandran et al, 1990;Rathod and Pandit, 2009). However there is practically no information available in the literature on the hydrodynamics of a sieve/nozzle plate column for the system like castor oil-water, which has different physico-chemical properties as compared with conventional systems.…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamics of Plate Column for Enzymatic Reactions of Oil

Dondge

Rathod

2015

Braz. J. Chem. Eng.

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-This work describes the hydrodynamics of a plate column of 5 cm inner diameter and 2.75 m height, operated in a semi-batch manner using an oil-water system especially important for enzymatic catalyzed reactions. The parameters such as dispersed phase superficial velocity, plate orifice size, number of nozzles, nozzle size and plate spacing, affecting the dispersed phase hold up, were investigated. It was observed that the orifice plate produced an uneven change in drop diameter and hence nozzles were used to study the hydrodynamics. The total and dynamic hold ups determined were increased with an increase in dispersed phase superficial velocity, while decreased with an increase in a nozzle size. The total hold up decreased, while dynamic hold up slightly increased with an increase in plate spacing. Correlations obtained are found to be appropriate for the estimation of the total hold up and dynamic hold up.

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Enzyme mass transfer coefficient in a sieve plate extraction column

Cited by 8 publications

References 21 publications

Xylanase mass transfer studies in aqueous two-phase systems using spray and sieve plate columns

Xylanase mass transfer studies in aqueous two-phase systems using spray and sieve plate columns

Flooding characteristics of aqueous two-phase systems in a countercurrent sieve-plate column

Hydrodynamics of Plate Column for Enzymatic Reactions of Oil

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